Protides of the Biological Fluids - 1st Edition - ISBN: 9780080279886, 9781483190327

Protides of the Biological Fluids, Volume 29

1st Edition

Proceedings of the Twenty-Ninth Colloquium, 1981

Editors: H. Peeters
eBook ISBN: 9781483190327
Imprint: Pergamon
Published Date: 1st January 1982
Page Count: 1010
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Table of Contents


Preface

Acknowledgments

The Tenth Arne Tiselius Memorial Lecture

H-Y Antigen and Sex Determination

Section A. Membrane Proteins

A.1. Pure Membrane Proteins

A.1.1. Purification Procedures

Some General Aspects of the Difficulties to Purify Membrane Proteins

New Application of Group-Specific Reagents for Hydrophobic Labeling and Crosslinking of Membrane Proteins

The Use of a Zwitterionic Detergent and a Monoclonal Antibody in Purification of Rat Liver 5'-Nucleotidase

A.1.2. Immunoglobulins

Human IgD, The Gene and the Protein

Structural Studies of Human IgD

Comparison of Secreted and Membrane-Bound Human Immunoglobulins M and D

A.1.3. Glycoproteins

The Sialoglycoproteins (Glycophorins) in the Plasma Membranes of Different Cells and Their Receptor Functions

Structures and Antigenic Properties of Human Erythrocyte Membrane Sialoglycoproteins

Cytoskeleton-Associated Surface Glycoproteins of Murine Lymphocytes

The Relationship Between Differentiation of Friend Erythroleukemia Cells and Synthesis of Plasma Membrane-Bound Glycoproteins

Variations in the Structure of Surface Glycoproteins Present in Different Lymphocyte Subpopulations

Enhanced Neuraminidase-Resistant Sialic Acid Content in the Surface Glycopeptides of Human Leukaemic Hairy Cells

Reconstitution of Liposomes Bearing Platelet Membrane Glycoproteins. A Functional Study with Von Willebrand Factor

Human Platelet Membrane Glycoproteins IIb and IIIa May Form Ca2+-Dependent Complexes

Glycoproteins of the Human Syncytiotrophoblastic Plasma Membrane

Characterization of α-Tissue Antigen (αTA), A Membrane Glycoprotein of Cancer Cells

The Structure and Function of a Membrane Glycoprotein Encoded in the Adenovirus Genome

Protein Micelles and Virosomes from the Surface Glycoproteins of Parainfluenza 3 Virus

A.1.4. Erythrocyte Membrane Proteins

Human Red Cell Membrane Acetylcholinesterase, A Model Enzyme for the Study of Protein-Amphiphile Interactions

Spectrin Free Vesicles Obtained from Human Erythrocytes Without ATP-Depletion

Acylation of Integral Erythrocyte Membrane Proteins Resulting in a Soluble Form of Band 3 Protein

Self-Association of Band 3 Protein from Erythrocyte Membranes in Solutions of a Nonionic Detergent, Ammonyx-LO

Interactions of the Isolated Spectrin Polypeptides (Bands 1 and 2) with Phospholipid Monolayers

Red Cell Membrane Changes in a Heinz Body Anaemia (Kale Anaemia)

Ozone-Induced Cross-Linking of Erythrocyte Membrane Proteins

A.1.5. Various Cell Membrane Proteins

Membrane Proteins of Murine T Cell Clones

Isolation and Characterization of Basal-Lateral and Luminal Plasma-Membranes (PM) from the Proximal Tubule of Human Kidney

Further Immunochemical Studies of Tissue Polypeptide Antigen (TPA)

Purification of Membrane 5'Nucleotidase Preparation and Properties of Anti-5'Nucleotidase Serum

Characterization of Four Chick Brain Synaptic Membrane Proteins

Isolation of a Membrane Antigen from Electric Organ of Torpedo Californica

Characterization of Some Antigens Associated with the Membrane of Hampster Tumor Cells

Non-Specific Binding of (-)3H-Dihydroalprenolol to Intact Human Lymphocytes and Lymphocyte Membranes

Characterization of (-) (3H) Dihydroalprenolol Binding Sites on Intact Human Peripheral Blood Lymphocytes

A.1.6. Bacterial Membrane Proteins

Structure and Function of a Pore-Forming Transmembrane Protein: High Resolution Studies of a Bacterial Porin

Evidence for Protein-Lipid Interactions in Membranes of Acholeplasma Laidlawii

Isolation of Surface Antigens from Schistosoma Mansoni Schistosomula

Crossed Immunoelectrophoretic Analysis of the Purple Membrane of Halobacterium Halobium

A.2. Cell Surface Antigens

A.2.1. Beta 2 Microglobulin

Beta-2-Microglobulin and its Association with Histocompatibility Antigens of the Mouse and Rabbit

Evolutionary Conservation of the Interaction Between ß2-Microglobulin and ß2-Microglobulin-Binding Molecules

Seminal Plasma Levels of Beta2-Microglobulin and CEA-Like Protein in Infertility

Stimulation of Human Monocyte Chemotaxis by Anti-ß2-Microglobulin

Exchange of ß2-Microglobulin in Histocompatibility Antigens by its Human Analogue

Rat Serum Components with Affinity for Human ß2 Microglobulin

A.2.2. Blood Group Antigens

Human Blood Group M-, N-, T (Thomsen-Friedenreich)-, and Tn-Specific Lipidic Substances in Line-10 Hepatocarcinoma of Strain-2 Guinea Pigs

Further Evidence that Terminal ß-Galactopyranosyl Groups are Immunodeterminant in Blood Group N Specificity

A.2.3. Histocompatibility Antigens

Biosynthesis of HLA-A and -B Antigens

Biochemical Characterisation of Leukaemia-Associated Cell Surface Antigens

Influence of Selective Absence of H-2KK on Gross Virus Leukaemias In Vitro and the Implications for In Vivo Surveillance of Leukaemia

Primary Structural Analyses of Murine Major Histocompatibility Antigens

Characterization of DLA SD Antigens Using Immunochemical Techniques

Neuron-Specific Markers: A Critical Appraisal

Variations in the Antigenic Architecture of the Acholeplasma Laidlawii Cell Membrane

Interspecies Crossreactivities of Antisera Directed Against (Na,K)-ATPase Subunits from Bufo Marinus

A.3. Membrane Function

A.3.1. Transport Function

Transport of Proteins into Mitochondria

The Stereospecific D-Glucose Transport Activity of Cholate Extracts from Human Erythrocyte Membranes. Effects of Cholate, EDTA and Dithioerythritol

Side-Specific Transfer of Membrane-Bound Phospholipase A2 to Other Membranes by Fusion

Bile Acid Transport in Distal Ileum

The Kinetics of Intramolecular Crosslinking of the Band 3 Protein by 4,4′-Diisothiocyanato Dihydrostilbene-2,2′,-Disulfonic Acid (H2DIDS)

The Effect of Arginine Specific Reagents on Anion Transport Across Red Blood Cells

Does Ternary Complex Formation Control the State of the Cation Barrier in the Resealed Human Red Blood Cell Ghost?

A.3.2. Cell Adhesion

Microfilaments and Fibronectin Fibers Involved in the Formation of Fibroblast's Focal Contacts

Cell Surface Glycoproteins and Fibronectin Mediated Cell Adhesion

The Actin Network in Human Granulocytes at Different Stages of Phagocytosis in Granulopathies

Expression of Fibronectin and Plasminogen Activator in Human Cell Hybrids

Role of Fibronectin Degradation Products on Cellular Transformation

A.3.3 Cell Growth

Possible Role of the Plasma Membrane in Regulating Cell Growth Behaviour

Infiltration of Lymphosarcoma Cells into hepatocyte Cultures: Inhibition by Univalent Antibodies Against Liver Plasma Membranes and Lymphosarcoma Cells

The Role of Components of the Outer Membrane of Gram-Negative Bacteria in the Serum-Bactericidal Effect

Receptor-Ligand Interaction Leading to Entry of Myxoviruses into Host Cells

A.3.4. Cell Ageing

Molecular Aging: A Termination Antigen Appears on Senescent Cells

K+ Fluxes During T Lymphocyte Activation in Human Aging

Age-Related Defect of Cytokine-Ca2+ -cGMP Mediated Transmembrane Signal in T Lymphocyte Activation

Supramolecular Assembly of Membrane Constituents During 'In Vitro' Aging of Human Erythrocytes

Masking of RCA I Binding Sites by Hyaluronic Acid in Foetal Liver Cells

Parallel Increase of 3-0-Methylglucose Uptake and Cytochalasin B Binding in Chick Fibroblasts During Embryo Development

Section B. Receptor Ligand Interactions

B.1. Structure Activity Relations

The Acetylcholine Receptor: An Allosteric Membrane Protein

Structure and Function of the Human Platelet Von Willebrand Factor Receptor

Studies on the Receptor Sites of the Monoclonal Anti-Pr and -Sa Cold Agglutinins

Relations Between Serum α1-Foetoprotein (AFP) and Uterine Cytosol Receptors in the Immature Rat: Importance of Fatty Acids Ligands

Specific Binding of the Apo and Holoforms of the Serum DBP to the 'Soluble' Part of Actin from Human Lymphocytes and Platelets

ACTH Effects on Steroidogenesis and Adrenal Protein Synthesis: Relation Structure-Function

Similarities of the Gaunine Nucleotide Binding Sites Responsible for Adenylate Cyclase Activation and Carbamylcholine Binding Inhibition, In Rat Heart Membranes

Studies on the Hydrophobic Anchor of the Pig Intestinal Intrinsic Factor Receptor

Receptor-Mediated Endocytosis of Glycosylated Derivatives of Bovine Serum Albumin: Targeting Based on Sugar Recognition

Structure-Activity-Relationships in the Steroidal 17ß - Carboxamide Series

Binding of Cathepsin B by Haptoglobin

B.2. Immunoglobulin Receptors

Regulation of the Immune Response by the Fc Portion of Immunoglobulin

Human T-Lymphocytes' Fc-Receptors with High Affinity for Monomeric IgG

Isolation of Fcγ Receptors Released by Human Lymphocytes

Cyanogen Bromide Fragments of Mouse IgG2a and IgG2b which Bind to their Fc Receptors on Mouse Macrophages

Anti-Receptor Antibodies: A Comparison in the Rat of the Plasma to Bile Transfer of Purified IgG, F(ab′)2 and Fab′ Antibodies Against Rat Secretory Component (SC)

Receptor-Mediated Endocytosis of Polymeric IgA by Cultured Rat Hepatocytes

A Neo-Antigen Present on Complex α2M, Related to the Receptor Recognition Site

Events Initiated by Soluble Immune Complex Interaction with Immunoglobulin Receptors on Macrophages

B.3. Complement Receptors

Molecular Weight Analysis of Human Complement (C3) Receptors Isolated by Immunoprecipitation

The Receptor Functions of Endogeneous C1q, A Subcomponent of the First Component of Complement, on Peritoneal Macrophages

Receptors for C3b, C3bi, C3d and ß1H on Lymphocytes and Phagocytic Cells

B.4. Transferrin and Heme Receptors

Evidence for Endocytosis of Iron Loaded Transferrin by Cultured Rat Embryo Fibroblasts

Transferrin Receptors in Transformation and Differentiation

Mediation of a Receptor Mechanism in the Uptake of Iron from Transferrin by the Hepatocyte

Iron Uptake by Chinese Hamster Fibroblasts from Human Transferrin

Receptor-Mediated Endocytosis of Heme-Hemopexin by Rat Hepatocytes

Spectral and Other Studies on the Intestinal Heme Receptor of the Pig

B.5. Neurochemical Receptors

Differentiation Between Stereospecific Binding Sites on a Dopamine Receptor Complex: Effects of Proteolytic Enzymes

Biochemical and Pharmacological Studies of Substance P Receptors

Neurotensin-Receptor Interactions: Binding and Pharmacological Studies; Biological Relevance; Structure-Activity Relationships

A Novel Triazolopyridazine, 3H-CL 218, 872 which Labels the Benzodiazepine Receptor in Rat Brain

Immunological Studies of ß Adrenergic Catecholamine Receptors: A Two-Way Approach

Asymmetry of Acetylcholinesterase and Acetylcholine Receptor in Intact Secretory Vesicles from Adrenal Medulla

Loss of ß2-Adrenoceptors After Haemophilus Influenzae Vaccination

Characterization of Adrenergic Binding in Canine Lung Tissue

B.6. Vasoactive Intestinal Polypeptides (VIP)

Heterogeneity of VIP-Secretin Receptors in Three Rat Tissues

Vasoactive Intestinal Peptide (VIP) Control of Cyclic AMP Accumulation in Digestive Epithelia: Involvement of Multiple Regulatory Processes

Effects of GTP on Vasopressin, and Angiotensin Binding to Rat Liver Membranes

Cholecystokinin in Brain and Hypophysis

Vasoactive Intestinal Polypeptide (VIP): Specific Receptors on Smooth Muscle Membranes from Porcine Uterus

Impaired Secretin-VIP Sensitivity of Adenylate Cyclase in the Heart of Congenitally Hypertensive (SHR) or Obese (fa/fa) Rats

B.7. Hormone Receptors

B.7.1. Insulin Receptors

Multiple Functions of Peptides: Insulin and Vasoactive Intestinal Polypeptide Bind to Receptors in Liver and Brain

The Complex Kinetics of Insulin Binding to Receptors on Isolated Pig Hepatocytes

In Vivo Studies by Gamma-Camera of 131I-Insulin Distribution and Metabolism in Rabbits

Immunohistochemical and Autoradiographic Visualization of Porcine and Human 125I-Insulin Hepatic Binding In Vivo

B.7.2. Alpha MSH

Variation of α-MSH Receptivity Between Malignant Melanocytes from Horizontal and Vertical Growth Phases in a Human Superficial Spreading Melanoma

125I-α-MSH Binding in Human Melanoma Cell Lines

DNA Replication and Production of Retroviral Markers in Human Melanoma Cultures Depends on Degree of Differentiation and on Hormonal Stimulation

Binding of Endogenous α-MSH to Malignant Melanocytes in Human Melanomas

Hormonal Screening in Patients Suffering Malignant Melanoma. A Preliminary Approach

B.7.3. Other Hormones

Antibodies Against TSH Receptor in Graves' Disease: Evaluation by Release of cAMP

Antibodies Against TSH Receptor in Graves' Disease: Relation Between cGMP/cAMP Release

Negative Controls of Dog Thyroid Adenylate Cyclase Cyclic AMP System

Rat Liver Glucocorticoid Receptor Purified in Presence of Molybdate

Induction of Prolactin Liver Receptors in the Male Rat

An Affinity Adsorbent for an Estrogen Binding Protein Rat α-Fetoprotein

B.8. Various Receptors

Receptors for Regulatory Peptides in Epithelial Gut Cancer

Receptors that Control Acid Secretion in the Isolated Cells from Mammalian Gastric Mucosa

Microbial Receptors on Human Kidney Epithelia, A Possible Clue in the Pathogenesis of Bacterial Interstitial Nephritis

Section C. Monoclonal Antibodies

C.1. Hybridoma Technology

Human-Mouse Hybridoma Cells Producing Antibodies

Production of Rat Monoclonal Antibodies with the LOU Rat Non Secreting IR983F Myeloma Cell Line

Anti-Pr, -Gd and Related Cold Agglutinins: Human Monoclonal Antibodies Against Neuraminyl Groups

Analysis of Carbohydrate Determinants on Membranes of Leukemic Leukocytes Using Naturally Occurring Monoclonal Human Antibodies (Cold Agglutinins)

A Study of Hybridoma Soft Agar Cloning

Evaluation of Alternative Screening and Selection Procedures in Hybridoma Technology

Comparison of Screening Techniques Related to the Production of Monoclonal Antibodies

A Semi-Automated Enzyme-Linked Immunosorbent Assay (ELISA) to Screen for Hybridoma Cultures Producing Antibody to Carcinoembryonic Antigen (CEA)

Selection of Myeloma Lines Suitable for Hybridoma

Individual Physical Properties of Mouse Monoclonal Antibodies

C.2. Definition of Lymphocytes Subsets

Re-Definition of Human T Cells by Monoclonal Antibodies

Biochemical Studies of Human T Cell Markers

Mouse Monoclonal Antibodies Defining Functional Human Lymphocyte Subsets

T-Cell Profile and Reactivity In Vitro During Immune Reconstitution Following Bone Marrow Transplantation

Interaction of Monoclonal Antibodies and Antigen-Specific T-Cell Factor in Regulation of an Immune Response

Microfluorometric Analysis of Five Monoclonal Antibodies Directed Against Mouse Thymocytes

Binding Characteristics of a Monoclonal Antibody Against Rabbit Thymocytes

Abnormal Helper/Suppressor T-Cell Distribution in Chronic Brucellosis in Humans

Monoclonal Antibodies Defining Rabbit Lymphocyte Subpopulations

The Use of Monoclonal Antibodies to Monitor T-Cell Sub-Populations After Human Cardiac Transplantation

Monoclonal Anti-B Cell Antibodies: Their Effect on Human Mixed Lymphocyte Reactions

Monoclonal Antibodies Against Human Thymocytes and T Lymphocytes

Monoclonal Antibodies Against Rat Thymocytes

C.3. Determination of Histocompatibility Antigens

Monoclonal Antibodies Against Human MHC Products

Biochemical Analysis of Human HLA-DR Molecules

Functional Analysis of Human Ia Subsets

Reactivity of Monoclonal Anti-HLA Antibodies with Blood Platelets

Subsets of Human D Locus Products Identified by a Series of Monoclonal Antibodies

C.4. Viral and Bacterial Detection

Monoclonal Antibodies Detecting Epstein-Barr Virus Strain and Group Specific Antigenic Determinants

Monoclonal Antibody Against EBV-Associated Transformation Antigen Detected In Vitro and In Vivo

Production of Monoclonal Antibodies Against Poliomyelitis and Rabies Viruses

A Set of Monoclonal Antibodies to Streptococcal Group Polysaccharide Antigens

Hybridoma Antibody Mediated Activation of ß-Galactosidases from Genetically Defective E. Coli

Characterization of Monoclonal Antibodies to Subunit A of Cholera Toxin

Production of Monoclonal Antibodies Against Mycoplasma Hyorhinis as a Secondary Effect of Immunisation of Mice with Cultured Cell Lines

C.5. Detection of Proteins

Monoclonal Antibodies to the Human Leukocyte Interferons and Their Use for Interferon Purification and a Quantitative Interferon Assay

Radioimmunoassay (RIA) of Post-Gamma Globulin Using a Monoclonal Antibody

Localization of Collagen Type IV in Basement Membranes by Monoclonal Antibodies

Study of α1 Microglobulin (HC Protein) with Monoclonal Antibodies

Anti-Idiotypic Monoclonal Antibodies Against Myasthenie Antibodies

Quantitation of IgGkappa and IgGlambda in Normal and Pathological Sera

Use of Monoclonal Antibodies in the Characterisation of Immunoglobulin Classes and Subclasses in Animal Species

Monoclonal Antibodies Directed Against a Torpedo Electric Organ Membrane Antigen

C.6. Identification of Lipids

Monoclonal Antibodies Against Membrane Glycolipids of an Anti-Streptococcal Group A B-Cell Hybridoma

Monoclonal Antibody Secreting Mouse Hybridoma Clones Express Different Sets of Membrane Glycosphingolipids in Different Antigenic Systems

Glycosphingolipid Patterns of Established Human Hematopoietic Cell Lines

Monoclonal Antibodies to Tumor-Associated Glycolipids: Preparation and Application for Suppression of Tumor Growth

Monoclonal Antibodies Against Human Milkfat Globule Membranes Detecting Differentiation Antigens of the Mammary Gland

C.7. Determination of Hormones

Monoclonal Antibodies in Clinical Diagnosis — Reagents for Detecting Hormones and Immunoglobulins

Production of Monoclonal Antibodies to Steroid Hormones

Monoclonal Antibodies Against Growth Hormone: Effects on the Hormone Interaction with Specific Cell Surface Receptors

Monoclonal Antibodies Against Human Growth Hormone

Characteristics of Monoclonal Antibodies Against Human Chorionic Gonadotrophin (HCG)

Sensitive and Specific RIA for Human Chorionic Gonadotropin (hCG) Using Monoclonal Antibodies

The Use of Monoclonal Antibodies Against Prolactin (Prl) in a Solid Phase Enzyme-Immunoassay (EIA)

Mouse Monoclonal Antibodies Against Human Prolactine

Monoclonal Antibodies to Human Prolactin and Chorionic Somatomammotropin

Monoclonal Thyroglobulin-Autoantibodies as a Tool to Study Autoimmunity

C.8. Determination of Enzymes

Monoclonal Antibodies Against Two Antigens of Clinical Interest

Monoclonal Antibodies Against Human Acid α-Glucosidase

High-Affinity Monoclonal Autoantibody Used as a Reagent in a RIA for Human Creatine Kinase BB

C.9. Detection of Cancer Antigens

Five Melanoma-Associated Antigens Identified by Monoclonal Antibodies

Antigen Expression and Immune Lysis of Human Melanoma Cells: Analysis with Monoclonal Antibodies

Reactions of Monoclonal Antibodies Against Human Melanoma with Different Tissues and Cell Lines

Molecular Heterogeneity of a High Molecular Weight Human Melanoma Associated Antigen (MAA) Detected by Monoclonal Antibodies

A Monoclonal Antibody Defining a Burkitt Lymphoma Associated Antigen

Preparation and Characterization of Monoclonal Antibodies Against Antigens of Human Renal Adenocarcinoma

Monoclonal Antibodies Against Carcinoembryonic Antigen (CEA)

Characterisation of Monoclonal Antibodies to Carcinoembryonic Antigen by Antibody Overlay of Polyacrylamide Slab Gels

C.10. Drug Targeting

Phase I Trials of Murine Monoclonal Antibodies to Tumor Associated Antigens: Preliminary Observations

The Potential Use of Monoclonal Antibodies in Drug Targeting

Specific Killing of Human and Mouse Tumor Cells by Immunotoxins

Drug Targeting with Monoclonal Antibodies

Analysis of Human Haemopoietic Precursor Cell Antigens Using Astatine Labelled Monoclonal Antibodies

Section D. New Methods

The IGS (Immuno Gold Staining) Method Used with Monoclonal Antibodies

Enumeration of T Lymphocytes and their Subclasses in Peripheral Blood by Immunogold Staining

Chromatofocusing: A New High Resolution Method for Protein Fractionation

The Multiwire Proportional Counter for the Study of Ligand-Binding of Serum Proteins

Identification of Specific Antigens or Antibodies After Electrophoretic Transfer. Application to Measles Virus

Author Index

Subject Index

Description

Protides of the Biological Fluids: An International Review Series Devoted to Proteins and Related Studies, Volume 29: Membrane Proteins, Receptor Ligand Interactions, Monoclonal Antibodies, New Methods focuses on membrane proteins, receptor-ligand interactions, and monoclonal proteins as a reagent.

The selection first offers information on H-Y and sex determination and aspects of the difficulties to purify membrane proteins. Topics include solubilization by acylation, influence of supporting media on the aggregation of proteins, solubilization with detergents and organic solvents, and membrane proteins - giant detergent molecules. The text then examines the application of group-specific reagents for hydrophobic labeling and crosslinking of membrane proteins and comparison of secreted and membrane-bound human immunoglobulins M and D.

The publication tackles sialoglycoproteins (glycophorins) in the plasma membranes of different cells and their receptor functions and the structures and antigenic properties of human erythrocyte membrane sialoglycoproteins. Discussions focus on the primary structure of human erythrocyte glycophorins, molecular structure of the MN bloodgroup substance, and genetic variants of glycophorin A. The book also takes a look at the glycoproteins of the human syncytiotrophoblastic plasma membrane and structure and function of a membrane glycoprotein encoded in the adenovirus genome.

The selection is a valuable reference for readers interested in membrane proteins, receptor ligand interactions, and monoclonal antibodies.


Details

No. of pages:
1010
Language:
English
Copyright:
© Pergamon 1982
Published:
Imprint:
Pergamon
eBook ISBN:
9781483190327

About the Editors

H. Peeters Editor