Protides of the Biological Fluids

Protides of the Biological Fluids

Proceedings of the Twenty-Ninth Colloquium, 1981

1st Edition - January 1, 1982

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  • Editor: H. Peeters
  • eBook ISBN: 9781483190327

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Protides of the Biological Fluids: An International Review Series Devoted to Proteins and Related Studies, Volume 29: Membrane Proteins, Receptor Ligand Interactions, Monoclonal Antibodies, New Methods focuses on membrane proteins, receptor-ligand interactions, and monoclonal proteins as a reagent. The selection first offers information on H-Y and sex determination and aspects of the difficulties to purify membrane proteins. Topics include solubilization by acylation, influence of supporting media on the aggregation of proteins, solubilization with detergents and organic solvents, and membrane proteins - giant detergent molecules. The text then examines the application of group-specific reagents for hydrophobic labeling and crosslinking of membrane proteins and comparison of secreted and membrane-bound human immunoglobulins M and D. The publication tackles sialoglycoproteins (glycophorins) in the plasma membranes of different cells and their receptor functions and the structures and antigenic properties of human erythrocyte membrane sialoglycoproteins. Discussions focus on the primary structure of human erythrocyte glycophorins, molecular structure of the MN bloodgroup substance, and genetic variants of glycophorin A. The book also takes a look at the glycoproteins of the human syncytiotrophoblastic plasma membrane and structure and function of a membrane glycoprotein encoded in the adenovirus genome. The selection is a valuable reference for readers interested in membrane proteins, receptor ligand interactions, and monoclonal antibodies.

Table of Contents

  • Preface


    The Tenth Arne Tiselius Memorial Lecture

    H-Y Antigen and Sex Determination

    Section A. Membrane Proteins

    A.1. Pure Membrane Proteins

    A.1.1. Purification Procedures

    Some General Aspects of the Difficulties to Purify Membrane Proteins

    New Application of Group-Specific Reagents for Hydrophobic Labeling and Crosslinking of Membrane Proteins

    The Use of a Zwitterionic Detergent and a Monoclonal Antibody in Purification of Rat Liver 5'-Nucleotidase

    A.1.2. Immunoglobulins

    Human IgD, The Gene and the Protein

    Structural Studies of Human IgD

    Comparison of Secreted and Membrane-Bound Human Immunoglobulins M and D

    A.1.3. Glycoproteins

    The Sialoglycoproteins (Glycophorins) in the Plasma Membranes of Different Cells and Their Receptor Functions

    Structures and Antigenic Properties of Human Erythrocyte Membrane Sialoglycoproteins

    Cytoskeleton-Associated Surface Glycoproteins of Murine Lymphocytes

    The Relationship Between Differentiation of Friend Erythroleukemia Cells and Synthesis of Plasma Membrane-Bound Glycoproteins

    Variations in the Structure of Surface Glycoproteins Present in Different Lymphocyte Subpopulations

    Enhanced Neuraminidase-Resistant Sialic Acid Content in the Surface Glycopeptides of Human Leukaemic Hairy Cells

    Reconstitution of Liposomes Bearing Platelet Membrane Glycoproteins. A Functional Study with Von Willebrand Factor

    Human Platelet Membrane Glycoproteins IIb and IIIa May Form Ca2+-Dependent Complexes

    Glycoproteins of the Human Syncytiotrophoblastic Plasma Membrane

    Characterization of α-Tissue Antigen (αTA), A Membrane Glycoprotein of Cancer Cells

    The Structure and Function of a Membrane Glycoprotein Encoded in the Adenovirus Genome

    Protein Micelles and Virosomes from the Surface Glycoproteins of Parainfluenza 3 Virus

    A.1.4. Erythrocyte Membrane Proteins

    Human Red Cell Membrane Acetylcholinesterase, A Model Enzyme for the Study of Protein-Amphiphile Interactions

    Spectrin Free Vesicles Obtained from Human Erythrocytes Without ATP-Depletion

    Acylation of Integral Erythrocyte Membrane Proteins Resulting in a Soluble Form of Band 3 Protein

    Self-Association of Band 3 Protein from Erythrocyte Membranes in Solutions of a Nonionic Detergent, Ammonyx-LO

    Interactions of the Isolated Spectrin Polypeptides (Bands 1 and 2) with Phospholipid Monolayers

    Red Cell Membrane Changes in a Heinz Body Anaemia (Kale Anaemia)

    Ozone-Induced Cross-Linking of Erythrocyte Membrane Proteins

    A.1.5. Various Cell Membrane Proteins

    Membrane Proteins of Murine T Cell Clones

    Isolation and Characterization of Basal-Lateral and Luminal Plasma-Membranes (PM) from the Proximal Tubule of Human Kidney

    Further Immunochemical Studies of Tissue Polypeptide Antigen (TPA)

    Purification of Membrane 5'Nucleotidase Preparation and Properties of Anti-5'Nucleotidase Serum

    Characterization of Four Chick Brain Synaptic Membrane Proteins

    Isolation of a Membrane Antigen from Electric Organ of Torpedo Californica

    Characterization of Some Antigens Associated with the Membrane of Hampster Tumor Cells

    Non-Specific Binding of (-)3H-Dihydroalprenolol to Intact Human Lymphocytes and Lymphocyte Membranes

    Characterization of (-) (3H) Dihydroalprenolol Binding Sites on Intact Human Peripheral Blood Lymphocytes

    A.1.6. Bacterial Membrane Proteins

    Structure and Function of a Pore-Forming Transmembrane Protein: High Resolution Studies of a Bacterial Porin

    Evidence for Protein-Lipid Interactions in Membranes of Acholeplasma Laidlawii

    Isolation of Surface Antigens from Schistosoma Mansoni Schistosomula

    Crossed Immunoelectrophoretic Analysis of the Purple Membrane of Halobacterium Halobium

    A.2. Cell Surface Antigens

    A.2.1. Beta 2 Microglobulin

    Beta-2-Microglobulin and its Association with Histocompatibility Antigens of the Mouse and Rabbit

    Evolutionary Conservation of the Interaction Between ß2-Microglobulin and ß2-Microglobulin-Binding Molecules

    Seminal Plasma Levels of Beta2-Microglobulin and CEA-Like Protein in Infertility

    Stimulation of Human Monocyte Chemotaxis by Anti-ß2-Microglobulin

    Exchange of ß2-Microglobulin in Histocompatibility Antigens by its Human Analogue

    Rat Serum Components with Affinity for Human ß2 Microglobulin

    A.2.2. Blood Group Antigens

    Human Blood Group M-, N-, T (Thomsen-Friedenreich)-, and Tn-Specific Lipidic Substances in Line-10 Hepatocarcinoma of Strain-2 Guinea Pigs

    Further Evidence that Terminal ß-Galactopyranosyl Groups are Immunodeterminant in Blood Group N Specificity

    A.2.3. Histocompatibility Antigens

    Biosynthesis of HLA-A and -B Antigens

    Biochemical Characterisation of Leukaemia-Associated Cell Surface Antigens

    Influence of Selective Absence of H-2KK on Gross Virus Leukaemias In Vitro and the Implications for In Vivo Surveillance of Leukaemia

    Primary Structural Analyses of Murine Major Histocompatibility Antigens

    Characterization of DLA SD Antigens Using Immunochemical Techniques

    Neuron-Specific Markers: A Critical Appraisal

    Variations in the Antigenic Architecture of the Acholeplasma Laidlawii Cell Membrane

    Interspecies Crossreactivities of Antisera Directed Against (Na,K)-ATPase Subunits from Bufo Marinus

    A.3. Membrane Function

    A.3.1. Transport Function

    Transport of Proteins into Mitochondria

    The Stereospecific D-Glucose Transport Activity of Cholate Extracts from Human Erythrocyte Membranes. Effects of Cholate, EDTA and Dithioerythritol

    Side-Specific Transfer of Membrane-Bound Phospholipase A2 to Other Membranes by Fusion

    Bile Acid Transport in Distal Ileum

    The Kinetics of Intramolecular Crosslinking of the Band 3 Protein by 4,4′-Diisothiocyanato Dihydrostilbene-2,2′,-Disulfonic Acid (H2DIDS)

    The Effect of Arginine Specific Reagents on Anion Transport Across Red Blood Cells

    Does Ternary Complex Formation Control the State of the Cation Barrier in the Resealed Human Red Blood Cell Ghost?

    A.3.2. Cell Adhesion

    Microfilaments and Fibronectin Fibers Involved in the Formation of Fibroblast's Focal Contacts

    Cell Surface Glycoproteins and Fibronectin Mediated Cell Adhesion

    The Actin Network in Human Granulocytes at Different Stages of Phagocytosis in Granulopathies

    Expression of Fibronectin and Plasminogen Activator in Human Cell Hybrids

    Role of Fibronectin Degradation Products on Cellular Transformation

    A.3.3 Cell Growth

    Possible Role of the Plasma Membrane in Regulating Cell Growth Behaviour

    Infiltration of Lymphosarcoma Cells into hepatocyte Cultures: Inhibition by Univalent Antibodies Against Liver Plasma Membranes and Lymphosarcoma Cells

    The Role of Components of the Outer Membrane of Gram-Negative Bacteria in the Serum-Bactericidal Effect

    Receptor-Ligand Interaction Leading to Entry of Myxoviruses into Host Cells

    A.3.4. Cell Ageing

    Molecular Aging: A Termination Antigen Appears on Senescent Cells

    K+ Fluxes During T Lymphocyte Activation in Human Aging

    Age-Related Defect of Cytokine-Ca2+ -cGMP Mediated Transmembrane Signal in T Lymphocyte Activation

    Supramolecular Assembly of Membrane Constituents During 'In Vitro' Aging of Human Erythrocytes

    Masking of RCA I Binding Sites by Hyaluronic Acid in Foetal Liver Cells

    Parallel Increase of 3-0-Methylglucose Uptake and Cytochalasin B Binding in Chick Fibroblasts During Embryo Development

    Section B. Receptor Ligand Interactions

    B.1. Structure Activity Relations

    The Acetylcholine Receptor: An Allosteric Membrane Protein

    Structure and Function of the Human Platelet Von Willebrand Factor Receptor

    Studies on the Receptor Sites of the Monoclonal Anti-Pr and -Sa Cold Agglutinins

    Relations Between Serum α1-Foetoprotein (AFP) and Uterine Cytosol Receptors in the Immature Rat: Importance of Fatty Acids Ligands

    Specific Binding of the Apo and Holoforms of the Serum DBP to the 'Soluble' Part of Actin from Human Lymphocytes and Platelets

    ACTH Effects on Steroidogenesis and Adrenal Protein Synthesis: Relation Structure-Function

    Similarities of the Gaunine Nucleotide Binding Sites Responsible for Adenylate Cyclase Activation and Carbamylcholine Binding Inhibition, In Rat Heart Membranes

    Studies on the Hydrophobic Anchor of the Pig Intestinal Intrinsic Factor Receptor

    Receptor-Mediated Endocytosis of Glycosylated Derivatives of Bovine Serum Albumin: Targeting Based on Sugar Recognition

    Structure-Activity-Relationships in the Steroidal 17ß - Carboxamide Series

    Binding of Cathepsin B by Haptoglobin

    B.2. Immunoglobulin Receptors

    Regulation of the Immune Response by the Fc Portion of Immunoglobulin

    Human T-Lymphocytes' Fc-Receptors with High Affinity for Monomeric IgG

    Isolation of Fcγ Receptors Released by Human Lymphocytes

    Cyanogen Bromide Fragments of Mouse IgG2a and IgG2b which Bind to their Fc Receptors on Mouse Macrophages

    Anti-Receptor Antibodies: A Comparison in the Rat of the Plasma to Bile Transfer of Purified IgG, F(ab′)2 and Fab′ Antibodies Against Rat Secretory Component (SC)

    Receptor-Mediated Endocytosis of Polymeric IgA by Cultured Rat Hepatocytes

    A Neo-Antigen Present on Complex α2M, Related to the Receptor Recognition Site

    Events Initiated by Soluble Immune Complex Interaction with Immunoglobulin Receptors on Macrophages

    B.3. Complement Receptors

    Molecular Weight Analysis of Human Complement (C3) Receptors Isolated by Immunoprecipitation

    The Receptor Functions of Endogeneous C1q, A Subcomponent of the First Component of Complement, on Peritoneal Macrophages

    Receptors for C3b, C3bi, C3d and ß1H on Lymphocytes and Phagocytic Cells

    B.4. Transferrin and Heme Receptors

    Evidence for Endocytosis of Iron Loaded Transferrin by Cultured Rat Embryo Fibroblasts

    Transferrin Receptors in Transformation and Differentiation

    Mediation of a Receptor Mechanism in the Uptake of Iron from Transferrin by the Hepatocyte

    Iron Uptake by Chinese Hamster Fibroblasts from Human Transferrin

    Receptor-Mediated Endocytosis of Heme-Hemopexin by Rat Hepatocytes

    Spectral and Other Studies on the Intestinal Heme Receptor of the Pig

    B.5. Neurochemical Receptors

    Differentiation Between Stereospecific Binding Sites on a Dopamine Receptor Complex: Effects of Proteolytic Enzymes

    Biochemical and Pharmacological Studies of Substance P Receptors

    Neurotensin-Receptor Interactions: Binding and Pharmacological Studies; Biological Relevance; Structure-Activity Relationships

    A Novel Triazolopyridazine, 3H-CL 218, 872 which Labels the Benzodiazepine Receptor in Rat Brain

    Immunological Studies of ß Adrenergic Catecholamine Receptors: A Two-Way Approach

    Asymmetry of Acetylcholinesterase and Acetylcholine Receptor in Intact Secretory Vesicles from Adrenal Medulla

    Loss of ß2-Adrenoceptors After Haemophilus Influenzae Vaccination

    Characterization of Adrenergic Binding in Canine Lung Tissue

    B.6. Vasoactive Intestinal Polypeptides (VIP)

    Heterogeneity of VIP-Secretin Receptors in Three Rat Tissues

    Vasoactive Intestinal Peptide (VIP) Control of Cyclic AMP Accumulation in Digestive Epithelia: Involvement of Multiple Regulatory Processes

    Effects of GTP on Vasopressin, and Angiotensin Binding to Rat Liver Membranes

    Cholecystokinin in Brain and Hypophysis

    Vasoactive Intestinal Polypeptide (VIP): Specific Receptors on Smooth Muscle Membranes from Porcine Uterus

    Impaired Secretin-VIP Sensitivity of Adenylate Cyclase in the Heart of Congenitally Hypertensive (SHR) or Obese (fa/fa) Rats

    B.7. Hormone Receptors

    B.7.1. Insulin Receptors

    Multiple Functions of Peptides: Insulin and Vasoactive Intestinal Polypeptide Bind to Receptors in Liver and Brain

    The Complex Kinetics of Insulin Binding to Receptors on Isolated Pig Hepatocytes

    In Vivo Studies by Gamma-Camera of 131I-Insulin Distribution and Metabolism in Rabbits

    Immunohistochemical and Autoradiographic Visualization of Porcine and Human 125I-Insulin Hepatic Binding In Vivo

    B.7.2. Alpha MSH

    Variation of α-MSH Receptivity Between Malignant Melanocytes from Horizontal and Vertical Growth Phases in a Human Superficial Spreading Melanoma

    125I-α-MSH Binding in Human Melanoma Cell Lines

    DNA Replication and Production of Retroviral Markers in Human Melanoma Cultures Depends on Degree of Differentiation and on Hormonal Stimulation

    Binding of Endogenous α-MSH to Malignant Melanocytes in Human Melanomas

    Hormonal Screening in Patients Suffering Malignant Melanoma. A Preliminary Approach

    B.7.3. Other Hormones

    Antibodies Against TSH Receptor in Graves' Disease: Evaluation by Release of cAMP

    Antibodies Against TSH Receptor in Graves' Disease: Relation Between cGMP/cAMP Release

    Negative Controls of Dog Thyroid Adenylate Cyclase Cyclic AMP System

    Rat Liver Glucocorticoid Receptor Purified in Presence of Molybdate

    Induction of Prolactin Liver Receptors in the Male Rat

    An Affinity Adsorbent for an Estrogen Binding Protein Rat α-Fetoprotein

    B.8. Various Receptors

    Receptors for Regulatory Peptides in Epithelial Gut Cancer

    Receptors that Control Acid Secretion in the Isolated Cells from Mammalian Gastric Mucosa

    Microbial Receptors on Human Kidney Epithelia, A Possible Clue in the Pathogenesis of Bacterial Interstitial Nephritis

    Section C. Monoclonal Antibodies

    C.1. Hybridoma Technology

    Human-Mouse Hybridoma Cells Producing Antibodies

    Production of Rat Monoclonal Antibodies with the LOU Rat Non Secreting IR983F Myeloma Cell Line

    Anti-Pr, -Gd and Related Cold Agglutinins: Human Monoclonal Antibodies Against Neuraminyl Groups

    Analysis of Carbohydrate Determinants on Membranes of Leukemic Leukocytes Using Naturally Occurring Monoclonal Human Antibodies (Cold Agglutinins)

    A Study of Hybridoma Soft Agar Cloning

    Evaluation of Alternative Screening and Selection Procedures in Hybridoma Technology

    Comparison of Screening Techniques Related to the Production of Monoclonal Antibodies

    A Semi-Automated Enzyme-Linked Immunosorbent Assay (ELISA) to Screen for Hybridoma Cultures Producing Antibody to Carcinoembryonic Antigen (CEA)

    Selection of Myeloma Lines Suitable for Hybridoma

    Individual Physical Properties of Mouse Monoclonal Antibodies

    C.2. Definition of Lymphocytes Subsets

    Re-Definition of Human T Cells by Monoclonal Antibodies

    Biochemical Studies of Human T Cell Markers

    Mouse Monoclonal Antibodies Defining Functional Human Lymphocyte Subsets

    T-Cell Profile and Reactivity In Vitro During Immune Reconstitution Following Bone Marrow Transplantation

    Interaction of Monoclonal Antibodies and Antigen-Specific T-Cell Factor in Regulation of an Immune Response

    Microfluorometric Analysis of Five Monoclonal Antibodies Directed Against Mouse Thymocytes

    Binding Characteristics of a Monoclonal Antibody Against Rabbit Thymocytes

    Abnormal Helper/Suppressor T-Cell Distribution in Chronic Brucellosis in Humans

    Monoclonal Antibodies Defining Rabbit Lymphocyte Subpopulations

    The Use of Monoclonal Antibodies to Monitor T-Cell Sub-Populations After Human Cardiac Transplantation

    Monoclonal Anti-B Cell Antibodies: Their Effect on Human Mixed Lymphocyte Reactions

    Monoclonal Antibodies Against Human Thymocytes and T Lymphocytes

    Monoclonal Antibodies Against Rat Thymocytes

    C.3. Determination of Histocompatibility Antigens

    Monoclonal Antibodies Against Human MHC Products

    Biochemical Analysis of Human HLA-DR Molecules

    Functional Analysis of Human Ia Subsets

    Reactivity of Monoclonal Anti-HLA Antibodies with Blood Platelets

    Subsets of Human D Locus Products Identified by a Series of Monoclonal Antibodies

    C.4. Viral and Bacterial Detection

    Monoclonal Antibodies Detecting Epstein-Barr Virus Strain and Group Specific Antigenic Determinants

    Monoclonal Antibody Against EBV-Associated Transformation Antigen Detected In Vitro and In Vivo

    Production of Monoclonal Antibodies Against Poliomyelitis and Rabies Viruses

    A Set of Monoclonal Antibodies to Streptococcal Group Polysaccharide Antigens

    Hybridoma Antibody Mediated Activation of ß-Galactosidases from Genetically Defective E. Coli

    Characterization of Monoclonal Antibodies to Subunit A of Cholera Toxin

    Production of Monoclonal Antibodies Against Mycoplasma Hyorhinis as a Secondary Effect of Immunisation of Mice with Cultured Cell Lines

    C.5. Detection of Proteins

    Monoclonal Antibodies to the Human Leukocyte Interferons and Their Use for Interferon Purification and a Quantitative Interferon Assay

    Radioimmunoassay (RIA) of Post-Gamma Globulin Using a Monoclonal Antibody

    Localization of Collagen Type IV in Basement Membranes by Monoclonal Antibodies

    Study of α1 Microglobulin (HC Protein) with Monoclonal Antibodies

    Anti-Idiotypic Monoclonal Antibodies Against Myasthenie Antibodies

    Quantitation of IgGkappa and IgGlambda in Normal and Pathological Sera

    Use of Monoclonal Antibodies in the Characterisation of Immunoglobulin Classes and Subclasses in Animal Species

    Monoclonal Antibodies Directed Against a Torpedo Electric Organ Membrane Antigen

    C.6. Identification of Lipids

    Monoclonal Antibodies Against Membrane Glycolipids of an Anti-Streptococcal Group A B-Cell Hybridoma

    Monoclonal Antibody Secreting Mouse Hybridoma Clones Express Different Sets of Membrane Glycosphingolipids in Different Antigenic Systems

    Glycosphingolipid Patterns of Established Human Hematopoietic Cell Lines

    Monoclonal Antibodies to Tumor-Associated Glycolipids: Preparation and Application for Suppression of Tumor Growth

    Monoclonal Antibodies Against Human Milkfat Globule Membranes Detecting Differentiation Antigens of the Mammary Gland

    C.7. Determination of Hormones

    Monoclonal Antibodies in Clinical Diagnosis — Reagents for Detecting Hormones and Immunoglobulins

    Production of Monoclonal Antibodies to Steroid Hormones

    Monoclonal Antibodies Against Growth Hormone: Effects on the Hormone Interaction with Specific Cell Surface Receptors

    Monoclonal Antibodies Against Human Growth Hormone

    Characteristics of Monoclonal Antibodies Against Human Chorionic Gonadotrophin (HCG)

    Sensitive and Specific RIA for Human Chorionic Gonadotropin (hCG) Using Monoclonal Antibodies

    The Use of Monoclonal Antibodies Against Prolactin (Prl) in a Solid Phase Enzyme-Immunoassay (EIA)

    Mouse Monoclonal Antibodies Against Human Prolactine

    Monoclonal Antibodies to Human Prolactin and Chorionic Somatomammotropin

    Monoclonal Thyroglobulin-Autoantibodies as a Tool to Study Autoimmunity

    C.8. Determination of Enzymes

    Monoclonal Antibodies Against Two Antigens of Clinical Interest

    Monoclonal Antibodies Against Human Acid α-Glucosidase

    High-Affinity Monoclonal Autoantibody Used as a Reagent in a RIA for Human Creatine Kinase BB

    C.9. Detection of Cancer Antigens

    Five Melanoma-Associated Antigens Identified by Monoclonal Antibodies

    Antigen Expression and Immune Lysis of Human Melanoma Cells: Analysis with Monoclonal Antibodies

    Reactions of Monoclonal Antibodies Against Human Melanoma with Different Tissues and Cell Lines

    Molecular Heterogeneity of a High Molecular Weight Human Melanoma Associated Antigen (MAA) Detected by Monoclonal Antibodies

    A Monoclonal Antibody Defining a Burkitt Lymphoma Associated Antigen

    Preparation and Characterization of Monoclonal Antibodies Against Antigens of Human Renal Adenocarcinoma

    Monoclonal Antibodies Against Carcinoembryonic Antigen (CEA)

    Characterisation of Monoclonal Antibodies to Carcinoembryonic Antigen by Antibody Overlay of Polyacrylamide Slab Gels

    C.10. Drug Targeting

    Phase I Trials of Murine Monoclonal Antibodies to Tumor Associated Antigens: Preliminary Observations

    The Potential Use of Monoclonal Antibodies in Drug Targeting

    Specific Killing of Human and Mouse Tumor Cells by Immunotoxins

    Drug Targeting with Monoclonal Antibodies

    Analysis of Human Haemopoietic Precursor Cell Antigens Using Astatine Labelled Monoclonal Antibodies

    Section D. New Methods

    The IGS (Immuno Gold Staining) Method Used with Monoclonal Antibodies

    Enumeration of T Lymphocytes and their Subclasses in Peripheral Blood by Immunogold Staining

    Chromatofocusing: A New High Resolution Method for Protein Fractionation

    The Multiwire Proportional Counter for the Study of Ligand-Binding of Serum Proteins

    Identification of Specific Antigens or Antibodies After Electrophoretic Transfer. Application to Measles Virus

    Author Index

    Subject Index

Product details

  • No. of pages: 1010
  • Language: English
  • Copyright: © Pergamon 1982
  • Published: January 1, 1982
  • Imprint: Pergamon
  • eBook ISBN: 9781483190327

About the Editor

H. Peeters

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