Protides of the Biological Fluids

Protides of the Biological Fluids

Proceedings of the Twenty-Sixth Colloquim, 1978

1st Edition - January 1, 1979

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  • Editor: H. Peeters
  • eBook ISBN: 9781483158020

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Protides of the Biological Fluids contains the proceedings of the 26th Colloquium in 1978. Two topics are highlighted, the first one being the immune complexes and the second one being the cytoskeletal proteins. This book is organized into three sections, namely, Immune Complexes, Cytoskeletal Proteins, and Techniques. The Immune Complexes section states the biochemical problems and answers some intriguing clinical problems in this field. This section details the theory of the antigen/antibody reaction, the isolation of complexes, and the nature of the antigen. The Cytoskeletal Proteins section discusses the theoretical aspect of cell structure. The last section describes the assay methods and individual protein assays.

Table of Contents

  • Contents



    The Seventh Arne Tiselius Memorial Lecture

    Are Circulating Immune Complexes The Key To Immunopathology?

    Section A. Immune Complexes

    A.1. Detection Methods and Reagents

    Pitfalls in the Use of Biologic Reagents For the Detection of Immune Complexes

    Preparation of Stabilized Human IgG Aggregates of Known Size and Their Reactivity in Immune Complex Assays

    Automated Determination of Circulating Immune Complexes by Pacia

    A Semi-Automated System Permitting Analysis of Immune Complex Components

    A New Solid Phase Assay System For Immune Complexes Using Fc-Fragments of Human IgG (Spaif)

    The Use of Bovine Conglutinin As A Reagent For Detecting Immune Complexes

    Detection of Circulating Immune Complexes by Radioimmunoassay with Monoclonal Rheumatoid Factor: Comparison with Clq Binding and Raji Cell Radioassays in Cancer

    Rheumatoid Factor Radioimmunoassay For Immune Complexes

    Detection of Immune Complexes in Human Renal Transplantation by Using Anti-Antibody

    Measurements of Immune Complex Formation by Difference Turbidity

    A Titration Method For Rf and Immune Complexes

    A.2. Theory of Antigen-Antibody Reaction

    Antibody Structures and Effector Functions: Immunoglobulin Carbohydrates Revisited

    The Alternative Pathway of Complement Activation: Initiation, Amplification and Control

    Functional Differentiation of Immunoglobulin G

    The Precipitin Reaction: The Importance of The Fc-Portion of IgG

    Calculation of Immune Complex Distributions

    Computer Modeling of Precipitin Reactions

    Modification of The Interaction Between Platelets and Immune Complexes by Cl Subcomponents (First Component of Complement)

    Light Scattering by Growing Antigen-Antibody Complexes

    A.3. Isolation of Immune Complexes

    Immunologie Properties of Aggregated Human IgG: Column Fractionation of Thermal Aggregates

    Comparison of Raji Cells and Protein A-Sepharose For Isolation of Soluble Immune Complexes From Serum

    Isolation of Soluble Immune Complexes by Affinity Chromatography Using Staphylococcal Protein-A Sepharose As Substrate

    Purification of Soluble Immune Complexes From Serum Using Solid-Phase Conglutinin and Clq

    A.4. Nature of Antigen in Immune Complexes

    Are Immune Complexes Immunoglobulin Aggregates?

    A Multi-Faceted Approach To Characterization and Isolation of Immune Complexes

    Milk Precipitins and Circulating Immune Complexes in Selective IgG Deficiency

    Soluble Immune Complexes in Subjects with Selective Iga Deficiency

    Immunoglobulin-Alkaline Phosphatase Complexes in Human Serum

    A.5. Fate of Immune Complexes

    Transport of Immune Complexes To Germinal Centers

    Structural Determinants For the Circulating Life-Times of Immunoglobulins and Immune Complexes

    Clearance of Soluble Immune Complexes and Aggregates

    A.6. Immune Complexes in Arthritis

    Analysis of Immune Complexes in Rheumatoid Arthritis

    Circulating Immune Complexes in Ankylosing Spondylitis

    Clq Reactive Immune Complexes in Sjogren's Syndrome

    Immune Complexes in Rheumatoid Arthritis : Correlations with Clinical Features and Effects of Gold

    Thrombocyte Aggregation by Sera and Antiglobulin Complexes From Patients with Rheumatoid Arthritis

    A.7. Immune Complexes in Viral, Bacterial, Parasitic Infections

    A.7.1. Viral Infections

    A.7.2. Bacterial Infections

    A.7.3. Parasitic Infections

    A.8. Immune Complexes in Systemic Lupus (SLE)

    Activity of Systemic Lupus Erythematosus (SLE) Followed by Circulating Immune Complexes

    The Heterogeneity of Immune Complexes in Disease

    The Occurrence and Significance of Circulating Anti-ds-DNA And Free DNA in SLE

    Detection of Immune Complexes in Connective Tissue Diseases: A Comparison of Three Radioassays

    The Occurrence of Immune Complexes in Patients with Multiple Sclerosis

    A.9. Immune Complexes in Nephritis

    Immune Complexes in Human Glomerulonephritis. A Study of 99 Cases Using A Clq Binding Assay

    Clearance and Glomerular Deposition of Circulating Immune Complexes

    Protein Transport in Glomeruli with Immune Complexes

    Clearance and Glomerular Localization of Well Defined Immune ComplexesEs

    Circulating Immune Complexes in Acute Rheumatic Fever and Acute Poststreptococcal Glomerulonephritis

    Role of HBsAg Immune Complexes in Glomerulonephritis of Essential Mixed Cryoglobulinemia (EMC)

    Detection of Circulating Immune Complexes with Clq Binding Test in Glomerulonephritis and Complement Profiles

    Immune Complex Binding To Human Glomerular Complement Receptors

    Clq Binding Activity of Sera From Renal Transplanted Patients Treated with Anti-Lymphocyte Globulins

    Clq Binding Immune Complexes and C3 Nephritic Factor (C3nef) in Hypocomplementemic Human Glomerulonephritis

    Occurrence of Iga in Tissues of Patients with Alcoholic Hepatic Disease, Anaphylactoid Purpura and Recurrent - Often Benign - Hematuria

    Application of Solid Phase Clq Assay in Sera From Nephropathic Patients

    Immune Complex Type Glomerulonephritis in Rabbit Induced by A Streptococcal Antigen

    Circulating Immune Complexes in Renal Transplant Recipients

    A. 10. Immune Complexes in Cancer

    Relevance of Circulating Immune Complexes in Cancer Sera

    Evaluation of Prognosis and Tumor Extent in Cancer Patients by Detecting Circulating Immune Complexes with The 125i-Clq-Binding Assay

    Serial Testing For Immune Complexes in Malignant Melanoma

    Detection, Isolation and Characterization of Immune Complexes in Malignant Melanoma

    Demonstration of Circulating Immune Complexes in Melanoma Patients by Means of A Granulocyte Fagocytosis Test

    Clinical Implications of Elevated Levels of Circulating Immune Complexes in Patients with Malignant Melanoma

    Circulating Immune Complexes in Nasopharyngeal Carcinoma Patients and Their Household Contacts

    Circulating Immune Complexes in Sera of Patients with Lung Cancer

    Studies On Identification of Antigens in Immune Complexes of Lung Cancer Patients

    Circulating Immune Complexes in Multiple Myeloma

    A.II. Clinical Screening For Immune Complexes

    Detection of Circulating Immune Complexes by The Ciq Binding Activity CLQBA) in Liver Diseases with Or Without HBsAg

    The Clq Deviation Test For the Detection of Circulating Immune Complexes, A 2-Year? Survey in A University Medical Center

    Clinical Value of Immune Complex Assays

    Methods of De-Complementation and The Detection of IC

    Cryoglobulins As Immune Complexes: An Immunochemical Study of Cryoprecipitates

    Thyroglobulin and Anti-Thyroglobulin Immune Complexes in Human Sera

    Serial Measurement of Circulating Immune Complexes in Leukemia

    A. 12. Therapy of Immune Complex Syndromes

    Intensive Plasmapheresis in Idiopathic Thrombocytopenic Purpura

    Monitoring of Plasmapheresis in Sle by Immune Complex Assays

    Plasmapheresis in the Management of Systemic Lupus Erythematosus: The Relevance of Tests For Circulating Immune Complexes

    The Effect of Plasmapheresis On Circulating Immune Complexes Measured by Raji-Cell and Indirect Granulocyte Phagocytosis Text (IGPT)

    Cryoglobulin-Associated Immune Complex Disease: Treatment with Ant Venom

    A.13. Immune Complexes Under Experimental Conditions

    Evidence For Immunopathology in Primate Pregnancies

    Effect of Anti-Proteoglycan Sera On Metabolic Functions of Calf Cartilage Cells in Vitro

    Immunologie Arterial Injury in Swine

    Clq Binding Immune Complexes in Genetically Selected Thymus Deficient Swan Mice Or Athymic Mice

    Localization of Immune Complexes Induced by Mercuric Chloride in the Rat. A Study of Various Organs

    Section B. Cytoskeletal Proteins

    B.1. Cytoskeletal Protein Systems

    B.1.1. The Cytoskeleton

    B.1.2. Actin, Profilin

    B.1.3. Tubulin

    B.2. The Role of The Cytoskeletal Proteins

    The Role of Contractile Filaments in Platelet Activation

    Are Microfilaments Involved in Secretion?

    The Role of Contractile and Cytoskeletal Proteins During Wound Healing and Tumor Cell Invasion

    Ac Tin, Tubulin and Á-Actinin Cocap with Surface Immunoglobulins In Mouse B-Lymphocytes

    Microfilamentous-Microtubular System, Motile Events and Insulin Release in the Pancreatic B-Cell

    Studies of The Induction and Maintenance of A Generalized Polarization of Plasma Membrane Components

    Cellular Dynamics of Neuronal Cells. The Organization of CSK in Neuroblastoma Cells

    Cytoskeletal Proteins and Their Function in Pulmonary Alveolar Interstitial Cells

    Fibrin Clot Rectractile Activity of Cultured Human Fibroblasts: Physiopathological Implications

    B.3. Regulation of Cytoskeletal Protein Systems

    Reaction of The Contractile Apparatus in Physarum to Injected Ca++, ATP, ADP and 5'AMP

    Dynamics of Calcium Movements in Pancreatic Islets: Their Relevance to the Mechanics of Insulin Release

    Analysis of Inner Face Plasma Membrane Proteins Involved in the Sensitivity of Na+/K+ Atpase To Ouabain

    B.4. Immunological Aspects of The Cytoskeleton

    Antigenic Similarities Between Intracellular 10 Nm Filaments of Fibroblasts and Extracellular Microfibrils

    Smooth Muscle Antibodies Reacting with Microtubular Antigens

    Immunomicroscopy of Cytoskeletal Filaments by Use of Purkinje Fibre Antiskeletin

    Antigens Common To Human Fibroblasts and Serum

    Section C. Techniques

    C.1. Assay Methods

    The Bioluminescent Determination of Substrates Using NAD-dependent Oxidoreductase Enzymes

    Labeling of Tryptophan Residues in Peptides

    Purification of Proteins by Subtraction Affinity Chromâtography

    Control of Unwanted Fluorescence in Immunohistology

    Kinetics of Multivalent Alkane-Protein Interactions On Hydrophobie Agaroses and Implications For the Multivalent Effector-Receptor Model

    Immobilised Enzymes As Laboratory Reagents: Modification of the ph Optimum of Immobilised Glucose Oxidase

    Effect of Polyethylene Glycol (Peg) On The Formation of Immune Complexes in the Region of Antibody Excess Measured by Nephelometric and Turbidimetric Methods

    An Investigation Into the Distribution of Radial Immunodiffusion Quality Control Data

    M.L. Salkie

    Continuous-Crossed-Line Immunoelectrophoresis. A Methodological Modification For Comparison of Reference Patterns in Crossed Immunoelectrophoresis

    C.2. Individual Protein Assays

    A 2-Site Immunoradiometric Assay For the Determination of A-Albumin

    Determination of Á-Albumin (GFA) in Cerebrospinal Fluid

    A Solid Phase Enzyme Immuno-Assay of Ferritin in the Evaluation of Leukemia Patients

    Microheterogeneity of Human Isoferritins Using Iron-59 Labeling. A New Purification Procedure

    Antibody Labeling by Means of 125I-Protem A

    Estimation of Immunoglobulin Concentrations in Serum Using Kinetic Measurements of The Immunoprecipitin Reaction

    IgG Subclasses in Pepsin Resistant Normal Human IgG

    Comparative Studies of Serum Immunoglobulins and Enzyme Activities For the Diagnosis of Liver Fibrosis

    Author Index

    Subject Index

Product details

  • No. of pages: 750
  • Language: English
  • Copyright: © Pergamon 1979
  • Published: January 1, 1979
  • Imprint: Pergamon
  • eBook ISBN: 9781483158020

About the Editor

H. Peeters

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