Protides of the Biological Fluids - 1st Edition - ISBN: 9780080178226, 9781483146409

Protides of the Biological Fluids

1st Edition

Proceedings of the Twenty-First Colloquium, Brugge, 1973

Editors: H. Peeters
eBook ISBN: 9781483146409
Imprint: Pergamon
Published Date: 1st January 1974
Page Count: 694
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Description

Protides of the Biological Fluids contains the proceedings of the Twenty-First Colloquium held at Brugge in 1973. The Colloquium covers topics on membrane proteins, proteinuria, and new techniques under which comes the automated nephelometric analysis of proteins. The book is organized into three sections according to the topics of the Colloquium. Section A, Membrane, discusses all aspects of membrane proteins including their isolation and solubilization, the nature of their lipid-protein interaction and the physical probes used for their characterization. Section B, Urinary Proteins, centers on proteinuria, electrophoretic and immunoelectrophoretic methods enabling characterization of renal disease, and properties of specific urinary proteins and enzymes. The last section describes two immunological methods for protein quantitation.

Table of Contents


Preface

Acknowledgments

The Second Arne Tiselius Memorial Lecture

Alpha, Beta, Gamma, Omega? The Past, Present and Future of Plasma Proteins

Section A. Membranes

A.1. Solubilization and Characterization of Membrane Proteins

A.1.1. Erythrocyte Membranes

A.1.2. Lymphocyte Membranes

A.1.3. Platelet Membranes

A.1.4. Other Membranes

A.2. Conformation and Structure of Membranes

Protein Distribution in Membranes

Recent Thermal and N.M.R. Studies of Lipids and Membrane Systems

Impurity Effects of Spin-Label Probes: an Evaluation using Monomolecular Film Techniques

Protein—Lipid Interactions of the Membrane-Penetrating MN-Glycoprotein from the Human Erythrocyte

Evidence for Protein Penetration in Mitochondrial Membrane Structure

Use of Multifunctional Dextran Derivatives as Reagents to Study Plasma Membrane Protein Distribution

A.3. Protein-Lipid Interaction in Membranes

Protein—Lipid Interactions in Red Cell Membranes

Spin Label Studies of the Influence of Peptides on the Organization of Lipids in Bilayers

Enzymes and Ions at Lipid Monolayers

Lipid-Protein Interactions in Human Plasma High-Density Lipoproteins

A Comparison of Lipoproteins Derived from CNS Myelin of the Normal and of the Mutant 'qq' Mouse

Protein Conformational Studies in Biological Membranes

A.4. Membrane-Bound Enzymes and Hormones

The Small-Intestinal Sucrase—Isomaltase Complex: Reconstitution of Sucrase—Isomaltase-dependent Sugar Transport with Black Lipid Membranes

Molecular Structure of Acetylcholinesterase

Structure of Membrane-Bound Acetylcholinesterase

The Influence of Lipid Environment on Membrane-Bound Enzymes in Yeast Mitochondria

Studies on Binding of the Direct Lytic Factor (DLF) of Cobra Venom {Naja naja) to Red Cell Membranes

Plasma Membrane Insulin Receptor Regulation by Genetic, Metabolic and Hormonal Factors

Properties of the Gonadotropin Receptor in Bovine Corpora Lutea

Optical and Enzymatic Effects of Growth Hormone Interaction with Plasma Membranes

A.5. Immunological Function of Membranes

Genetic Control of the Induction of the Antibody Response

Antigenicity of Human Erythrocyte Glycoproteins

Comparison of θ-Alloantigen and Mouse T-Lymphocyte Specific Xenoantigen (MTLA)

Membrane Antigens Specific for Dividing Human T or Bλ Lymphoid Cells

Shedding of Lymphocytes Coated with Radio-Labeled Anti-HL-A

Demonstration of Surface-IG on Thymus Cells

Immunological Studies of Plasma Membrane Fractions Isolated from Normal and Cancerous Human Gastric Mucosa

Immunological Study of Plasma Membrane of Human Gastric Mucosa

Presence of an SV 40 Coded Antigen on the Surface of Different Cell Lines Transformed by this Virus

Membrane Proteins and Complement Action

Section B. Urinary Proteins

B.1. Proteinuria

B.1.1. Pathology

B.1.2. Electrophoretic Studies

B.1.3. Plasma and Urinary Protein Levels

B.2. Glomerular Clearance of Proteins

Anomalies of IgG Clearance in Focal Glomerulosclerosis

Studies on the Clinical Value of Protein Clearance in Renal Disease

A Study of the Glomerular Protein Leak Using Non-protein Macromolecules

Glomerular Proteinuria: Comparison of Immunological and Morphological Criteria

Urinary Albumin Excretion and Dextran Clearance in the Development of Diabetic Glomerulosclerosis

Purification of Basement Membrane Antigens from Normal Human Urine by Affinity Chromatography

Immunochemical Characterization of Human Glomerular Basement Membrane

B.3. Tubular Proteinuria

Differential Metabolism of Tubular Proteins

Structure and Function of the Retinol-Binding Protein: a Protein Characteristic of Tubular Proteinuria

Specific Tubular Histuria following Renal Transplantation

Proximal Tubule Fluid Protein Concentrations in Normal and Anti-GBM Nephritic Rats

Sequential Development of Tubular Proteinuria in Balkan Nephropathy

Low Molecular Weight Proteins in Tubular Proteinuria

B.4. Secretory Proteins

Renal Handling of ß2 -Microglobulin

The Urinary Excretion of ß2-Microglobulin (ß2µ) in Renal Disorders

The Cerebrospinal Fluid Microglobulins

Characterization and Immunochemical Quantitation of Human Uromucoid

Uromucoid Excretion by Normal Individuals and Stone-Formers

Isoelectric Focusing Studies on the Distribution of Uroproteins with Plasminogen Activator Activity

Urinary Excretion of Lysosomal Enzymes in Essential Hypertension

New Isoenzymes of Acid Hydrolases in Human Urine

Urinary Lipids in Proteinuria

Preparation and Characterization of Urinary Sialoglycopeptide and Sialooligosaccharide with Phytochemagglutinin Inhibitory Activity on Cell Growth

Section C: Techniques

C.1. Automated Nephelometric Analysis

Theory and Practice of Automated Nephelometry

The Effects of Different Polymers for Enhancement of the Antigen-Antibody Reaction as Measured with Nephelometry

A New Technique for the Immunoassay of Haptens: Nephelometric Inhibition Immunoassay (NINIA)

Automated Nephelometric Analysis of Specific Serum Proteins: Clinical Applications

Automated Nephelometric Analysis of Low Density Lipoproteins

A Convenient Method for the Study of Anticomplementary Substances in Biological Fluids

C.2. Radioimmunoassay

New Trends in Radioimmunoassay

A Method to Increase the Usable Life of Radioiodine-Labeled LH

Radioimmunoassay in Non-Equilibrium Conditions

Radioimmunological Detection of HB Antigen by an Automated Method

Big and Small Gastrins in Human Serum

C.3. New Techniques

The Measurement of Protein-Binding using Dried Polyacrylamide

Combined Use of Electroendosmosis-Free Agarose Gel and Polyacrylamide Gel in Some Electrophoretic Procedures

Gel Porosity and Chain Radius are Obtained from Chromatography by Using the Geometrical Gel Model

Analytical Isotachophoresis and Gel Electrofocusing of Synthetic Peptides

UV-Detection of Both Absorbing and Non-Absorbing Ions in Analytical Isotachophoresis

Author Index

Subject Index

Details

No. of pages:
694
Language:
English
Copyright:
© Pergamon 1974
Published:
Imprint:
Pergamon
eBook ISBN:
9781483146409

About the Editor

H. Peeters