Protides of the Biological Fluids

Protides of the Biological Fluids

Proceedings of the Twenty-First Colloquium, Brugge, 1973

1st Edition - January 1, 1974

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  • Editor: H. Peeters
  • eBook ISBN: 9781483146409

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Protides of the Biological Fluids contains the proceedings of the Twenty-First Colloquium held at Brugge in 1973. The Colloquium covers topics on membrane proteins, proteinuria, and new techniques under which comes the automated nephelometric analysis of proteins. The book is organized into three sections according to the topics of the Colloquium. Section A, Membrane, discusses all aspects of membrane proteins including their isolation and solubilization, the nature of their lipid-protein interaction and the physical probes used for their characterization. Section B, Urinary Proteins, centers on proteinuria, electrophoretic and immunoelectrophoretic methods enabling characterization of renal disease, and properties of specific urinary proteins and enzymes. The last section describes two immunological methods for protein quantitation.

Table of Contents

  • Preface


    The Second Arne Tiselius Memorial Lecture

    Alpha, Beta, Gamma, Omega? The Past, Present and Future of Plasma Proteins

    Section A. Membranes

    A.1. Solubilization and Characterization of Membrane Proteins

    A.1.1. Erythrocyte Membranes

    A.1.2. Lymphocyte Membranes

    A.1.3. Platelet Membranes

    A.1.4. Other Membranes

    A.2. Conformation and Structure of Membranes

    Protein Distribution in Membranes

    Recent Thermal and N.M.R. Studies of Lipids and Membrane Systems

    Impurity Effects of Spin-Label Probes: an Evaluation using Monomolecular Film Techniques

    Protein—Lipid Interactions of the Membrane-Penetrating MN-Glycoprotein from the Human Erythrocyte

    Evidence for Protein Penetration in Mitochondrial Membrane Structure

    Use of Multifunctional Dextran Derivatives as Reagents to Study Plasma Membrane Protein Distribution

    A.3. Protein-Lipid Interaction in Membranes

    Protein—Lipid Interactions in Red Cell Membranes

    Spin Label Studies of the Influence of Peptides on the Organization of Lipids in Bilayers

    Enzymes and Ions at Lipid Monolayers

    Lipid-Protein Interactions in Human Plasma High-Density Lipoproteins

    A Comparison of Lipoproteins Derived from CNS Myelin of the Normal and of the Mutant 'qq' Mouse

    Protein Conformational Studies in Biological Membranes

    A.4. Membrane-Bound Enzymes and Hormones

    The Small-Intestinal Sucrase—Isomaltase Complex: Reconstitution of Sucrase—Isomaltase-dependent Sugar Transport with Black Lipid Membranes

    Molecular Structure of Acetylcholinesterase

    Structure of Membrane-Bound Acetylcholinesterase

    The Influence of Lipid Environment on Membrane-Bound Enzymes in Yeast Mitochondria

    Studies on Binding of the Direct Lytic Factor (DLF) of Cobra Venom {Naja naja) to Red Cell Membranes

    Plasma Membrane Insulin Receptor Regulation by Genetic, Metabolic and Hormonal Factors

    Properties of the Gonadotropin Receptor in Bovine Corpora Lutea

    Optical and Enzymatic Effects of Growth Hormone Interaction with Plasma Membranes

    A.5. Immunological Function of Membranes

    Genetic Control of the Induction of the Antibody Response

    Antigenicity of Human Erythrocyte Glycoproteins

    Comparison of θ-Alloantigen and Mouse T-Lymphocyte Specific Xenoantigen (MTLA)

    Membrane Antigens Specific for Dividing Human T or Bλ Lymphoid Cells

    Shedding of Lymphocytes Coated with Radio-Labeled Anti-HL-A

    Demonstration of Surface-IG on Thymus Cells

    Immunological Studies of Plasma Membrane Fractions Isolated from Normal and Cancerous Human Gastric Mucosa

    Immunological Study of Plasma Membrane of Human Gastric Mucosa

    Presence of an SV 40 Coded Antigen on the Surface of Different Cell Lines Transformed by this Virus

    Membrane Proteins and Complement Action

    Section B. Urinary Proteins

    B.1. Proteinuria

    B.1.1. Pathology

    B.1.2. Electrophoretic Studies

    B.1.3. Plasma and Urinary Protein Levels

    B.2. Glomerular Clearance of Proteins

    Anomalies of IgG Clearance in Focal Glomerulosclerosis

    Studies on the Clinical Value of Protein Clearance in Renal Disease

    A Study of the Glomerular Protein Leak Using Non-protein Macromolecules

    Glomerular Proteinuria: Comparison of Immunological and Morphological Criteria

    Urinary Albumin Excretion and Dextran Clearance in the Development of Diabetic Glomerulosclerosis

    Purification of Basement Membrane Antigens from Normal Human Urine by Affinity Chromatography

    Immunochemical Characterization of Human Glomerular Basement Membrane

    B.3. Tubular Proteinuria

    Differential Metabolism of Tubular Proteins

    Structure and Function of the Retinol-Binding Protein: a Protein Characteristic of Tubular Proteinuria

    Specific Tubular Histuria following Renal Transplantation

    Proximal Tubule Fluid Protein Concentrations in Normal and Anti-GBM Nephritic Rats

    Sequential Development of Tubular Proteinuria in Balkan Nephropathy

    Low Molecular Weight Proteins in Tubular Proteinuria

    B.4. Secretory Proteins

    Renal Handling of ß2 -Microglobulin

    The Urinary Excretion of ß2-Microglobulin (ß2µ) in Renal Disorders

    The Cerebrospinal Fluid Microglobulins

    Characterization and Immunochemical Quantitation of Human Uromucoid

    Uromucoid Excretion by Normal Individuals and Stone-Formers

    Isoelectric Focusing Studies on the Distribution of Uroproteins with Plasminogen Activator Activity

    Urinary Excretion of Lysosomal Enzymes in Essential Hypertension

    New Isoenzymes of Acid Hydrolases in Human Urine

    Urinary Lipids in Proteinuria

    Preparation and Characterization of Urinary Sialoglycopeptide and Sialooligosaccharide with Phytochemagglutinin Inhibitory Activity on Cell Growth

    Section C: Techniques

    C.1. Automated Nephelometric Analysis

    Theory and Practice of Automated Nephelometry

    The Effects of Different Polymers for Enhancement of the Antigen-Antibody Reaction as Measured with Nephelometry

    A New Technique for the Immunoassay of Haptens: Nephelometric Inhibition Immunoassay (NINIA)

    Automated Nephelometric Analysis of Specific Serum Proteins: Clinical Applications

    Automated Nephelometric Analysis of Low Density Lipoproteins

    A Convenient Method for the Study of Anticomplementary Substances in Biological Fluids

    C.2. Radioimmunoassay

    New Trends in Radioimmunoassay

    A Method to Increase the Usable Life of Radioiodine-Labeled LH

    Radioimmunoassay in Non-Equilibrium Conditions

    Radioimmunological Detection of HB Antigen by an Automated Method

    Big and Small Gastrins in Human Serum

    C.3. New Techniques

    The Measurement of Protein-Binding using Dried Polyacrylamide

    Combined Use of Electroendosmosis-Free Agarose Gel and Polyacrylamide Gel in Some Electrophoretic Procedures

    Gel Porosity and Chain Radius are Obtained from Chromatography by Using the Geometrical Gel Model

    Analytical Isotachophoresis and Gel Electrofocusing of Synthetic Peptides

    UV-Detection of Both Absorbing and Non-Absorbing Ions in Analytical Isotachophoresis

    Author Index

    Subject Index

Product details

  • No. of pages: 694
  • Language: English
  • Copyright: © Pergamon 1974
  • Published: January 1, 1974
  • Imprint: Pergamon
  • eBook ISBN: 9781483146409

About the Editor

H. Peeters

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