Protein Phosphorylation, Part B, Volume 201

1st Edition

Analysis of Protein Phosphorylation, Protein Kinase Inhibitors, and Protein Phosphatases

Serial Volume Editors: Tony Hunter Bartholomew Sefton
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821029
eBook ISBN: 9780080883168
Imprint: Academic Press
Published Date: 6th August 1991
Page Count: 547
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Table of Contents

Analysis of Protein Phosphorylation: Phosphoamino Acid Determination: D.P. Ringer, Separation of Phosphotyrosine, Phosphoserine, and Phosphothreonine by High-Performance Liquid Chromatography. B. Duclos, S. Marcandier, and A.J. Cozzone, Chemical Properties and Separation of Phosphoamino Acids by Thin-Layer Chromatography and/or Electrophoresis. M.P. Kamps, Determination of Phosphoamino Acid Composition by Acid Hydrolysis of Protein Blotted to Immobilon. L.M. Kozma, A.J. Rossomando, and M.J. Weber, A Comparison of Three Methods for Detecting Tyrosine-Phosphorylated Proteins. Antibodies to Phosphoamino Acids: Immunoprecipitation and Immunoblotting: D. Heffetz, M. Fridkin, and Y. Zick, Generation and Use of Antibodies to Phosphothreonine. J.Y.J. Wang, Generation and Use of Antiphosphotyrosine Antibodies Raisedagainst Bacterially Expressed abl Protein. M.F. White and J.M. Backer, Preparation and Use of Antiphosphotyrosine Antibodies to Study Structure and Function of Insulin Receptor. A.R. Frackelton, Jr., M. Posner, B. Kannan, and F. Mermelstein, Generation of Monoclonal Antibodies against Phosphotyrosine and Their Use for Affinity Purification of Phosphotyrosine-Containing Proteins. J.R. Glenney, Isolation of Tyrosine-Phosphorylated Proteins and Generation of Monoclonal Antibodies. M.P. Kamps, Generation and Use of Antiphosphotyrosine Antibodies for Immunoblotting. Phosphopeptide Mapping and Phosphorylation Site Determination: W.J. Boyle, P. van der Geer, and T. Hunter, Phosphopeptide Mapping and Phosphoamino Acid Analysis by Two-Dimensional Separation on Thin-Layer Cellulose Plates. K. Luo, T.R. Hurley, and B.M. Sefton, Cyanogen Bromide Cleavage and Proteolytic Peptide Mapping of Proteins Immobilized to Membranes. P. Cohen, B.W. Gibson, and C.F.B. Holmes, In Vivo Phosphorylation States of Proteins: Fast Atom7079v, cont.Bombardment Mass Spectrometry and Other Techniques. H.E. Meyer, E. Hoffmann-Posorske, and L.M.G. Heilmeyer, Jr., Determination and Location of Pho


This volume provides a compilation of recent methods for studying protein phosphorylation.


Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.


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© Academic Press 1991
Academic Press
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About the Serial Volume Editors

Tony Hunter Serial Volume Editor

Tony Hunter received his Ph.D. in 1969 from the University of Cambridge, England. He joined the Salk Institute in 1975 as an assistant professor and has been a professor since 1982. His current interests are the protein-tyrosine kinases of the Src and growth factor receptor families and the protein-tyrosine phosphatases that remove the phosphates added by protein-tyrosine kinases. He was elected as a Fellow of the Royal Society of London in 1987, a Fellow of the American Academy of Arts and Sciences in 1992, and as an Associate Member of the European Molecular Biology Organization in 1992.

Affiliations and Expertise

The Salk Institute, La Jolla, California, USA

Bartholomew Sefton Serial Volume Editor

Affiliations and Expertise

The Salk Institute, San Diego, California, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA