Protein Phosphorylation, Part B, Volume 201

1st Edition

Analysis of Protein Phosphorylation, Protein Kinase Inhibitors, and Protein Phosphatases

Serial Volume Editors: Tony Hunter Bartholomew Sefton
Hardcover ISBN: 9780121821029
eBook ISBN: 9780080883168
Imprint: Academic Press
Published Date: 6th August 1991
Page Count: 547

Institutional Access


Table of Contents

Analysis of Protein Phosphorylation: Phosphoamino Acid Determination: D.P. Ringer, Separation of Phosphotyrosine, Phosphoserine, and Phosphothreonine by High-Performance Liquid Chromatography. B. Duclos, S. Marcandier, and A.J. Cozzone, Chemical Properties and Separation of Phosphoamino Acids by Thin-Layer Chromatography and/or Electrophoresis. M.P. Kamps, Determination of Phosphoamino Acid Composition by Acid Hydrolysis of Protein Blotted to Immobilon. L.M. Kozma, A.J. Rossomando, and M.J. Weber, A Comparison of Three Methods for Detecting Tyrosine-Phosphorylated Proteins. Antibodies to Phosphoamino Acids: Immunoprecipitation and Immunoblotting: D. Heffetz, M. Fridkin, and Y. Zick, Generation and Use of Antibodies to Phosphothreonine. J.Y.J. Wang, Generation and Use of Antiphosphotyrosine Antibodies Raised**against Bacterially Expressed abl Protein. M.F. White and J.M. Backer, Preparation and Use of Antiphosphotyrosine Antibodies to Study Structure and Function of Insulin Receptor. A.R. Frackelton, Jr., M. Posner, B. Kannan, and F. Mermelstein, Generation of Monoclonal Antibodies against Phosphotyrosine and Their Use for Affinity Purification of Phosphotyrosine-Containing Proteins. J.R. Glenney, Isolation of Tyrosine-Phosphorylated Proteins and Generation of Monoclonal Antibodies. M.P. Kamps, Generation and Use of Antiphosphotyrosine Antibodies for Immunoblotting. Phosphopeptide Mapping and Phosphorylation Site Determination: W.J. Boyle, P. van der Geer, and T. Hunter, Phosphopeptide Mapping and Phosphoamino Acid Analysis by Two-Dimensional Separation on Thin-Layer Cellulose Plates. K. Luo, T.R. Hurley, and B.M. Sefton, Cyanogen Bromide Cleavage and Proteolytic Peptide Mapping of Proteins Immobilized to Membranes. P. Cohen, B.W. Gibson, and C.F.B. Holmes, In Vivo Phosphorylation States of Proteins: Fast Atom7079v, cont.**Bombardment Mass Spectrometry and Other Techniques. H.E. Meyer, E. Hoffmann-Posorske, and L.M.G. Heilmeyer, Jr., Determination and Location of Pho

Description

This volume provides a compilation of recent methods for studying protein phosphorylation.

Readership

Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.


Details

No. of pages:
547
Language:
English
Copyright:
© Academic Press 1991
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883168
Hardcover ISBN:
9780121821029

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY


About the Serial Volume Editors

Tony Hunter Serial Volume Editor

Tony Hunter received his Ph.D. in 1969 from the University of Cambridge, England. He joined the Salk Institute in 1975 as an assistant professor and has been a professor since 1982. His current interests are the protein-tyrosine kinases of the Src and growth factor receptor families and the protein-tyrosine phosphatases that remove the phosphates added by protein-tyrosine kinases. He was elected as a Fellow of the Royal Society of London in 1987, a Fellow of the American Academy of Arts and Sciences in 1992, and as an Associate Member of the European Molecular Biology Organization in 1992.

Affiliations and Expertise

The Salk Institute, La Jolla, California, USA

Bartholomew Sefton Serial Volume Editor

Affiliations and Expertise

The Salk Institute, San Diego, California, U.S.A.