Protein Engineering for Therapeutics, Part B, Volume 503

1st Edition

Serial Volume Editors: K. Dane Wittrup Gregory L. Verdine
Hardcover ISBN: 9780123969620
eBook ISBN: 9780123972644
Imprint: Academic Press
Published Date: 19th January 2012
Page Count: 392
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Table of Contents

Volume in series  


Stapled Peptides for Intracellular Drug Targets

1. Introduction

2. All-Hydrocarbon Stapled α-Helical Peptides

3. The Design of Stapled Peptides

4. Stapled Peptide Synthesis

5. Olefin Metathesis

6. N-terminal and Internal Modifications

7. Cleavage of Stapled Peptides from the Solid Support

8. Stapled Peptide Purification

9. Biophysical Characterization

10. Cell Permeability

11. In Vitro Target Interaction and Activity Assays

12. In Vivo Efficacy

13. Strategies for Stapled Peptide Optimization

14. Summary


Mapping of Vascular ZIP Codes by Phage Display

1. Introduction

2. Methods

3. Concluding Remarks and Perspectives


Engineering Cyclic Peptide Toxins

1. Introduction

2. Peptide Design

3. Peptide Synthesis

4. Structural Analysis

5. Stability Assays

6. Special Considerations for Cyclic Peptides


Peptide Discovery Using Bacterial Display and Flow Cytometry

1. Introduction

2. Protocols

3. Conclusions


Designed Ankyrin Repeat Proteins (DARPins)

1. Introduction

2. Applications of DARPins

3. Protocols for DARPins in Biomedical Applications


Target-Binding Proteins Based on the 10th Human Fibronectin Type III Domain (10Fn3)

1. Introduction

2. Library Design

3. Choice of Selection Platform

4. Phage Display, mRNA Display, and Yeast-Surface Display of 10Fn3-Based Libraries

5. Production

6. Conclusion



1. Introduction

2. Cloning and Expression of Lipocalins and Antica


This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds

Key Features

  • Chapters provide an invaluable resource for academics, researchers and students alike
  • Iinternational board of authors
  • This volume is split into sections that cover subjects such as peptides, and scaffolds


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


No. of pages:
© Academic Press 2012
Academic Press
eBook ISBN:
Hardcover ISBN:

About the Serial Volume Editors

K. Dane Wittrup Serial Volume Editor

Gregory L. Verdine Serial Volume Editor