Secure CheckoutPersonal information is secured with SSL technology.
Free ShippingFree global shipping
No minimum order.
Volume in series
Stapled Peptides for Intracellular Drug Targets
2. All-Hydrocarbon Stapled α-Helical Peptides
3. The Design of Stapled Peptides
4. Stapled Peptide Synthesis
5. Olefin Metathesis
6. N-terminal and Internal Modifications
7. Cleavage of Stapled Peptides from the Solid Support
8. Stapled Peptide Purification
9. Biophysical Characterization
10. Cell Permeability
11. In Vitro Target Interaction and Activity Assays
12. In Vivo Efficacy
13. Strategies for Stapled Peptide Optimization
Mapping of Vascular ZIP Codes by Phage Display
3. Concluding Remarks and Perspectives
Engineering Cyclic Peptide Toxins
2. Peptide Design
3. Peptide Synthesis
4. Structural Analysis
5. Stability Assays
6. Special Considerations for Cyclic Peptides
Peptide Discovery Using Bacterial Display and Flow Cytometry
Designed Ankyrin Repeat Proteins (DARPins)
2. Applications of DARPins
3. Protocols for DARPins in Biomedical Applications
Target-Binding Proteins Based on the 10th Human Fibronectin Type III Domain (10Fn3)
2. Library Design
3. Choice of Selection Platform
4. Phage Display, mRNA Display, and Yeast-Surface Display of 10Fn3-Based Libraries
2. Cloning and Expression of Lipocalins and Anticalins in E. coli
3. Construction of a Genetic Anticalin Library
4. Preparation and Selection of a Phage Display Library for Anticalins
5. Preparation and Selection of a Bacterial Surface Display Library for Anticalins
6. Colony Screening for Anticalins with Specific Target-Binding Activity
7. Screening for Anticalins with Specific Target-Binding Activity Using Microtiter Plate Expression in E. coli
8. Measuring Target Affinity of Anticalins in an ELISA
9. Measuring Target Affinity of Anticalins via Surface Plasmon Resonance
10. Application of Anticalins in Biochemical Research and Drug Development
T Cell Receptor Engineering
2. Stability and Affinity Engineering of T Cell Receptors by Yeast Surface Display
3. Affinity Engineering and Selection of T Cell Receptors by T Cell Display
4. Expression, Purification, and Applications of Soluble scTv Proteins
5. Recipes for Media and Buffers
Engineering Knottins as Novel Binding Agents
2. Knottins as Scaffolds for Engineering Molecular Recognition
3. Engineering Knottins by Yeast Surface Display
4. Knottin Library Construction
5. Screening Yeast-Displayed Knottin Libraries
6. Knottin production by chemical synthesis or recombinant expression
7. Cell binding assays
Practical Theoretic Guidance for the Design of Tumor-Targeting Agents
2. What Molecular Size Is Best for Tumor Uptake?
3. Will Targeting Increase Nanoparticle Accumulation in a Tumor?
4. How Does Affinity Affect Biodistribution?
5. What Dose Is Necessary in Order to Overcome the “Binding Site Barrier”?
Reengineering Biopharmaceuticals for Targeted Delivery Across the Blood–Brain Barrier
2. Blood–Brain Barrier Receptor-Mediated Transport and Molecular Trojan Horses
3. Reengineering Recombinant Proteins for Targeted Brain Delivery
4. Genetic Engineering of Expression Plasmid DNA Encoding IgG Fusion Proteins
5. Pharmacokinetics and Brain Uptake of IgG Fusion Proteins
6. CNS Pharmacological Effects of IgG Fusion Proteins
7. Immune Response Against IgG Fusion Proteins
Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells
This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds
- Chapters provide an invaluable resource for academics, researchers and students alike
- Iinternational board of authors
- This volume is split into sections that cover subjects such as peptides, and scaffolds
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- No. of pages:
- © Academic Press 2012
- 19th January 2012
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Elsevier.com visitor survey
We are always looking for ways to improve customer experience on Elsevier.com.
We would like to ask you for a moment of your time to fill in a short questionnaire, at the end of your visit.
If you decide to participate, a new browser tab will open so you can complete the survey after you have completed your visit to this website.
Thanks in advance for your time.