Protein Engineering for Therapeutics, Part B

Protein Engineering for Therapeutics, Part B

1st Edition - January 19, 2012
This is the Latest Edition
  • Editors: K. Dane Wittrup, Gregory L. Verdine
  • Hardcover ISBN: 9780123969620
  • eBook ISBN: 9780123972644

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Description

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds

Key Features

  • Chapters provide an invaluable resource for academics, researchers and students alike
  • Iinternational board of authors
  • This volume is split into sections that cover subjects such as peptides, and scaffolds

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Volume in series  

    Preface

    Stapled Peptides for Intracellular Drug Targets

    1. Introduction

    2. All-Hydrocarbon Stapled α-Helical Peptides

    3. The Design of Stapled Peptides

    4. Stapled Peptide Synthesis

    5. Olefin Metathesis

    6. N-terminal and Internal Modifications

    7. Cleavage of Stapled Peptides from the Solid Support

    8. Stapled Peptide Purification

    9. Biophysical Characterization

    10. Cell Permeability

    11. In Vitro Target Interaction and Activity Assays

    12. In Vivo Efficacy

    13. Strategies for Stapled Peptide Optimization

    14. Summary

    Acknowledgments

    Mapping of Vascular ZIP Codes by Phage Display

    1. Introduction

    2. Methods

    3. Concluding Remarks and Perspectives

    Acknowledgments

    Engineering Cyclic Peptide Toxins

    1. Introduction

    2. Peptide Design

    3. Peptide Synthesis

    4. Structural Analysis

    5. Stability Assays

    6. Special Considerations for Cyclic Peptides

    Acknowledgments

    Peptide Discovery Using Bacterial Display and Flow Cytometry

    1. Introduction

    2. Protocols

    3. Conclusions

    Acknowledgments

    Designed Ankyrin Repeat Proteins (DARPins)

    1. Introduction

    2. Applications of DARPins

    3. Protocols for DARPins in Biomedical Applications

    Acknowledgment

    Target-Binding Proteins Based on the 10th Human Fibronectin Type III Domain (10Fn3)

    1. Introduction

    2. Library Design

    3. Choice of Selection Platform

    4. Phage Display, mRNA Display, and Yeast-Surface Display of 10Fn3-Based Libraries

    5. Production

    6. Conclusion

    Acknowledgments

    Anticalins

    1. Introduction

    2. Cloning and Expression of Lipocalins and Anticalins in E. coli

    3. Construction of a Genetic Anticalin Library

    4. Preparation and Selection of a Phage Display Library for Anticalins

    5. Preparation and Selection of a Bacterial Surface Display Library for Anticalins

    6. Colony Screening for Anticalins with Specific Target-Binding Activity

    7. Screening for Anticalins with Specific Target-Binding Activity Using Microtiter Plate Expression in E. coli

    8. Measuring Target Affinity of Anticalins in an ELISA

    9. Measuring Target Affinity of Anticalins via Surface Plasmon Resonance

    10. Application of Anticalins in Biochemical Research and Drug Development

    T Cell Receptor Engineering

    1. Introduction

    2. Stability and Affinity Engineering of T Cell Receptors by Yeast Surface Display

    3. Affinity Engineering and Selection of T Cell Receptors by T Cell Display

    4. Expression, Purification, and Applications of Soluble scTv Proteins

    5. Recipes for Media and Buffers

    Acknowledgments

    Engineering Knottins as Novel Binding Agents

    1. Introduction

    2. Knottins as Scaffolds for Engineering Molecular Recognition

    3. Engineering Knottins by Yeast Surface Display

    4. Knottin Library Construction

    5. Screening Yeast-Displayed Knottin Libraries

    6. Knottin production by chemical synthesis or recombinant expression

    7. Cell binding assays

    8. Summary

    Acknowledgments

    Practical Theoretic Guidance for the Design of Tumor-Targeting Agents

    1. Introduction

    2. What Molecular Size Is Best for Tumor Uptake?

    3. Will Targeting Increase Nanoparticle Accumulation in a Tumor?

    4. How Does Affinity Affect Biodistribution?

    5. What Dose Is Necessary in Order to Overcome the “Binding Site Barrier”?

    6. Conclusions

    Reengineering Biopharmaceuticals for Targeted Delivery Across the Blood–Brain Barrier

    1. Introduction

    2. Blood–Brain Barrier Receptor-Mediated Transport and Molecular Trojan Horses

    3. Reengineering Recombinant Proteins for Targeted Brain Delivery

    4. Genetic Engineering of Expression Plasmid DNA Encoding IgG Fusion Proteins

    5. Pharmacokinetics and Brain Uptake of IgG Fusion Proteins

    6. CNS Pharmacological Effects of IgG Fusion Proteins

    7. Immune Response Against IgG Fusion Proteins

    8. Summary

    Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells

    1. Introduction

    2. Methods

    3. Conclusion

Product details

  • No. of pages: 392
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: January 19, 2012
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123969620
  • eBook ISBN: 9780123972644

About the Serial Volume Editors

K. Dane Wittrup

Gregory L. Verdine