Description

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds

Key Features

  • Chapters provide an invaluable resource for academics, researchers and students alike
  • Iinternational board of authors
  • This volume is split into sections that cover subjects such as peptides, and scaffolds
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

    Table of Contents

    Volume in series  

    Preface

    Stapled Peptides for Intracellular Drug Targets

    1. Introduction

    2. All-Hydrocarbon Stapled α-Helical Peptides

    3. The Design of Stapled Peptides

    4. Stapled Peptide Synthesis

    5. Olefin Metathesis

    6. N-terminal and Internal Modifications

    7. Cleavage of Stapled Peptides from the Solid Support

    8. Stapled Peptide Purification

    9. Biophysical Characterization

    10. Cell Permeability

    11. In Vitro Target Interaction and Activity Assays

    12. In Vivo Efficacy

    13. Strategies for Stapled Peptide Optimization

    14. Summary

    Acknowledgments

    Mapping of Vascular ZIP Codes by Phage Display

    1. Introduction

    2. Methods

    3. Concluding Remarks and Perspectives

    Acknowledgments

    Engineering Cyclic Peptide Toxins

    1. Introduction

    2. Peptide Design

    3. Peptide Synthesis

    4. Structural Analysis

    5. Stability Assays

    6. Special Considerations for Cyclic Peptides

    Acknowledgments

    Peptide Discovery Using Bacterial Display and Flow Cytometry

    1. Introduction

    2. Protocols

    3. Conclusions

    Acknowledgments

    Designed Ankyrin Repeat Proteins (DARPins)

    1. Introduction

    2. Applications of DARPins

    3. Protocols for DARPins in Biomedical Applications

    Acknowledgment

    Target-Binding Proteins Based on the 10th Human Fibronectin Type III Domain (10Fn3)

    1. Introduction

    2. Library Design

    3. Choice of Selection Platform

    4. Phage Display, mRNA Display, and Yeast-Surface Display of 10Fn3-Based Libraries

    5. Production

    6. Conclusion

    Acknowledgments

    Anticalins

    1. Introduction

    2. Cloning and Expression of Lipocalins and Antica

    Details

    No. of pages:
    392
    Language:
    English
    Copyright:
    © 2012
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780123969620
    Electronic ISBN:
    9780123972644