Description

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds

Key Features

  • Chapters provide an invaluable resource for academics, researchers and students alike
  • Iinternational board of authors
  • This volume is split into sections that cover subjects such as peptides, and scaffolds

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Volume in series  

Preface

Stapled Peptides for Intracellular Drug Targets

1. Introduction

2. All-Hydrocarbon Stapled α-Helical Peptides

3. The Design of Stapled Peptides

4. Stapled Peptide Synthesis

5. Olefin Metathesis

6. N-terminal and Internal Modifications

7. Cleavage of Stapled Peptides from the Solid Support

8. Stapled Peptide Purification

9. Biophysical Characterization

10. Cell Permeability

11. In Vitro Target Interaction and Activity Assays

12. In Vivo Efficacy

13. Strategies for Stapled Peptide Optimization

14. Summary

Acknowledgments

Mapping of Vascular ZIP Codes by Phage Display

1. Introduction

2. Methods

3. Concluding Remarks and Perspectives

Acknowledgments

Engineering Cyclic Peptide Toxins

1. Introduction

2. Peptide Design

3. Peptide Synthesis

4. Structural Analysis

5. Stability Assays

6. Special Considerations for Cyclic Peptides

Acknowledgments

Peptide Discovery Using Bacterial Display and Flow Cytometry

1. Introduction

2. Protocols

3. Conclusions

Acknowledgments

Designed Ankyrin Repeat Proteins (DARPins)

1. Introduction

2. Applications of DARPins

3. Protocols for DARPins in Biomedical Applications

Acknowledgment

Target-Binding Proteins Based on the 10th Human Fibronectin Type III Domain (10Fn3)

1. Introduction

2. Library Design

3. Choice of Selection Platform

4. Phage Display, mRNA Display, and Yeast-Surface Display of 10Fn3-Based Libraries

5. Production

6. Conclusion

Acknowledgments

Anticalins

1. Introduction

2. Cloning and Expression of Lipocalins and Antica

Details

No. of pages:
392
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
eBook ISBN:
9780123972644
Print ISBN:
9780123969620

About the serial-volume-editors