Principles of Cloning

2nd Edition

Editors: Jose Cibelli Ian Wilmut Rudolf Jaenisch John Gurdon Robert Lanza Michael West Keith Campbell
Hardcover ISBN: 9780123865410
eBook ISBN: 9780123865427
Imprint: Academic Press
Published Date: 28th October 2013
Page Count: 572
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  Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species—including mice, sheep, cattle, and non-mammals—is considered as well.
  The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas.

Key Features

  • First and most comprehensive book on animal cloning, 100% revised
  • Describes an in-depth analysis of current limitations of the technology and research areas to explore
  • Offers cloning applications on basic biology, agriculture, biotechnology, and medicine


Bioengineers, biochemists, cell biologists, bone biologists, and geneticists.

Table of Contents

Editorial Advisory Board

Foreword to the Second Edition


Foreword to the First Edition

Preface to the First Edition

In remembrance of Keith Henry Stockman Campbell (23 May 1954–5 October 2012)

List of Contributors

Part I: Basics

Chapter 1. Artificial Activation of Mammalian Oocytes for Cloning: Present Status and Future Perspectives


Egg Activation Protocols

Common Activation Procedures for Mouse Eggs

Common Activation Procedures for Bovine and Porcine Eggs

Common Activation Procedures for Human Eggs




Chapter 2. Epigenetic Reprogramming of Somatic Nuclei via Cell Fusion


Epigenetic Reprogramming of Somatic Cells by Hybridization with Embryonic Stem Cells

Epigenetic Reprogramming of Somatic Cells by Hybridization with Embryonic Germ Cells

Role of Cell Fusion In Vivo

Cloning by Somatic Cell Transplantation into Oocytes

Molecular Mechanisms of Epigenetic Reprogramming Mediated by Cell Fusion



Chapter 3. Nuclear Origins and Clone Phenotype: What Cloning Tells Us about Embryonic Development


Aberrant Properties of Cloned Embryos

Effects of Developmental Stage of Donor Nucleus

Effects of Cell Type of Donor Nucleus

Effects of Donor Nucleus Cell Cycle Stage

Effects of Recipient Cell Type and Stage

Effects of Genotype and Sex of Donor Nucleus

Effects of Species Origin of Donor and Recipient – Challenges of Inter-Species Nuclear Transfer

Remediation of Aberrant Properties

Mysteries yet to be Solved



Part II: Methods

Chapter 4. Micromanipulation Techniques for Cloning


Making Manip


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Academic Press
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About the Editor

Jose Cibelli

Dr. Jose Cibelli is internationally recognized as one of the pioneers in the area of cloning for the production of animals and embryonic stem cells. Dr. Cibelli and his colleagues were responsible for the generation of the world's first transgenic cloned calves, the first stem cells by nuclear transfer in bovine, and the first embryonic stem cells by parthenogenesis in primates. His work has been published in numerous scientific journals including Science, Nature Biotechnology, Nature Medicine, Nature Methods, PNAS, and JAMA. He has testified about cloning in public forums sponsored by the US Food and Drug administration, the USA National Academy of Sciences, Canadian House of Commons, the USA Department of Agriculture, the United Nations Commission for Human Rights and the British Royal Society. He is currently Professor of Animal Biotechnology at Michigan State University, USA.

Affiliations and Expertise

Cellular Reprogramming Laboratory Michigan State University Ann Arbor, MI, USA

Ian Wilmut

Affiliations and Expertise

Director, MRC Centre for Regenerative Medicine, Edinburgh, UK

Rudolf Jaenisch

Affiliations and Expertise

Whitehead Institute Cambridge, MA, USA

John Gurdon

Affiliations and Expertise

The Wellcome Trust/ Cancer Research UK Cambridge, UK

Robert Lanza

Robert Lanza, M.D. is currently Chief Scientific Officer at Advanced Cell Technology, and Adjunct Professor of Surgical Sciences at Wake Forest University School of Medicine. He has several hundred scientific publications and patents, and over 30 books, including Principles of Tissue Engineering (1st through 4th Editions), Methods of Tissue Engineering, Principles of Cloning (1st and 2nd Editions), Essentials of Stem Cell Biology (1st and 2nd Editions), XENO, Yearbook of Cell & Tissue Transplantation, One World: The Health & Survival of the Human Species in the 21st Century (as editor, with forewords by C. Everett Koop and former President Jimmy Carter), and Medical Science & the Advancement of World Health. Dr. Lanza received his B.A. and M.D. degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. He is a former Fulbright Scholar, and studied as a student in the laboratory of Richard Hynes (MIT), Jonas Salk (The Salk Institute), and Nobel laureates Gerald Edelman (Rockefeller University) and Rodney Porter (Oxford University). He also worked closely (and coauthored a series of papers) with the late Harvard psychologist B.F. Skinner and heart transplant pioneer Christiaan Barnard. Dr. Lanza's current area of research focuses on the use of stem cells in regenerative medicine.

Affiliations and Expertise

Advanced Cell Technology, MA, USA

Michael West

Affiliations and Expertise

BioTime Inc. Alameda, CA, USA

Keith Campbell

Affiliations and Expertise

The University of Nottingham, UK