Practical Tissue Culture Applications - 1st Edition - ISBN: 9780124702851, 9780323150170

Practical Tissue Culture Applications

1st Edition

Editors: Karl Maramorosch
eBook ISBN: 9780323150170
Imprint: Academic Press
Published Date: 28th January 1979
Page Count: 442
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Practical Tissue Culture Applications contains the proceedings of a conference held at the International Laboratory for Research on Animal Diseases in Nairobi, Kenya, August 24-29, 1978. This book aims to describe some of the more important practical applications of in vitro techniques in a simple, easily understandable manner. Organized into three sections, with a total of 27 chapters, this book provides critical reviews, describes various techniques, and presents complete step-by-step methodology. It emphasizes applications pertaining to the health and economy in developing nations. In particular, this book discusses the pitfalls in preparing general purpose culture media, balanced salt solutions, and the procedures followed in the development of modern in vitro techniques. It also describes techniques for cultivation of vertebrate cells and organs; plant tissue culture and its numerous applications; and electron microscopy of cultured cell. This book explains as well virus isolation and identification in cell cultures, mass production of cells for vaccines, and use of cultured cells for drug evaluation. The applications of in vitro techniques to parasitology are explored in numerous chapters of this book.
Considering the potential benefit of application of in vitro techniques, this reference material will be of interest both in developed and developing countries.

Table of Contents



Section I Introduction to In Vitro Techniques

Chapter 1 Balanced Salt Solutions and General Purpose Culture Media

Chapter 2 Vertebrate Cell Culture: An Overview

Chapter 3 Plant Tissue Culture and Its Importance to Agriculture

Chapter 4 Common Problems in Tissue Culture Work in Developing Countries

Section II Applications of In Vitro Techniques

Chapter 5 Preparation of Cultured Cells for Electron Microscopy

Chapter 6 Isolation and Identification of Human Viruses

Chapter 7 Vertebrate and Invertebrate Cell Systems for the Isolation and Identification of Arboviruses

Chapter 8 The Application of Environmental Control to Continuous Culture and Vaccine Production

Chapter 9 Roller-Bottle Anchored Cell Cultures for the Production of Foot-and-Mouth Disease Purified Viral and Protein Vaccines

Chapter 10 Immunologic Assay for Malnutrition

Chapter 11 Culturing Lymphocytes for the Assessment of Immune Function and the Action of Antilymphocytic Agents

Chapter 12 Use of Tissue Culture to Detect Substances with Antiviral or Antitumor Activity

Chapter 13 Tissue Culture Applications for Plant Quarantine

Chapter 14 Animal and Plant Tissue Culture Decontamination

Section III Cultivation of Parasites

Chapter 15 Consideration of Electrolytes and Nutritional Components for the Propagation of Obligate Intracellular Agents in Cell Cultures

Chapter 16 Propagation of Theileria

Chapter 17 Techniques for in Vitro Cultivation of Erythrocytic and Exoerythrocytic States of Malarial Parasites

Chapter 18 Continuous in Vitro Cultivation of Plasmodium falciparum in Human Erythrocytes: Description of a Simple Technique to Obtain High Yields of Parasites

Chapter 19 In Vitro Cultivation of Plasmodium vivax and Plasmodium malariae

Chapter 20 Immunological Studies on Schistosomes Cultured in Vitro

Chapter 21 Cultivation of Salivarian Trypanosomes: Application to in Vitro Studies of African Trypanosomiases

Chapter 22 Methods in Mosquito Cell Culture

Chapter 23 Trends in Tick Cell Culture

Chapter 24 Tsetse Fly Tissue Culture and Its Application to the Propagation of African Trypanosomes in Vitro

Chapter 25 Biological Control of Insect Pests with Viruses

Chapter 26 Combination of in Vitro Feeding Techniques and Tissue Culture for Study of Arthropod-Borne Disease Agents

Section IV Future Prospects

Chapter 27 Future Prospects of Tissue Culture in Microbiology



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© Academic Press 1979
Academic Press
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About the Editor

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

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