Practical Tissue Culture Applications contains the proceedings of a conference held at the International Laboratory for Research on Animal Diseases in Nairobi, Kenya, August 24-29, 1978. This book aims to describe some of the more important practical applications of in vitro techniques in a simple, easily understandable manner.
Organized into three sections, with a total of 27 chapters, this book provides critical reviews, describes various techniques, and presents complete step-by-step methodology. It emphasizes applications pertaining to the health and economy in developing nations. In particular, this book discusses the pitfalls in preparing general purpose culture media, balanced salt solutions, and the procedures followed in the development of modern in vitro techniques. It also describes techniques for cultivation of vertebrate cells and organs; plant tissue culture and its numerous applications; and electron microscopy of cultured cell. This book explains as well virus isolation and identification in cell cultures, mass production of cells for vaccines, and use of cultured cells for drug evaluation. The applications of in vitro techniques to parasitology are explored in numerous chapters of this book.
Considering the potential benefit of application of in vitro techniques, this reference material will be of interest both in developed and developing countries.
Contributors Preface Section I Introduction to In Vitro Techniques Chapter 1 Balanced Salt Solutions and General Purpose Culture Media Chapter 2 Vertebrate Cell Culture: An Overview Chapter 3 Plant Tissue Culture and Its Importance to Agriculture Chapter 4 Common Problems in Tissue Culture Work in Developing Countries Section II Applications of In Vitro Techniques Chapter 5 Preparation of Cultured Cells for Electron Microscopy Chapter 6 Isolation and Identification of Human Viruses Chapter 7 Vertebrate and Invertebrate Cell Systems for the Isolation and Identification of Arboviruses Chapter 8 The Application of Environmental Control to Continuous Culture and Vaccine Production Chapter 9 Roller-Bottle Anchored Cell Cultures for the Production of Foot-and-Mouth Disease Purified Viral and Protein Vaccines Chapter 10 Immunologic Assay for Malnutrition Chapter 11 Culturing Lymphocytes for the Assessment of Immune Function and the Action of Antilymphocytic Agents Chapter 12 Use of Tissue Culture to Detect Substances with Antiviral or Antitumor Activity Chapter 13 Tissue Culture Applications for Plant Quarantine Chapter 14 Animal and Plant Tissue Culture Decontamination Section III Cultivation of Parasites Chapter 15 Consideration of Electrolytes and Nutritional Components for the Propagation of Obligate Intracellular Agents in Cell Cultures Chapter 16 Propagation of Theileria Chapter 17 Techniques for in Vitro Cultivation of Erythrocytic and Exoerythrocytic States of Malarial Parasites Chapter 18 Continuous in Vitro Cultivation of Plasmodium falciparum in Human Erythrocytes: Description of a Simple Technique to Obtain High Yields of Parasites Chapter 19 In Vitro Cultivation of Plasmodium vivax and Plasmodium malariae Chapter 20 Immunological Studies on Schistosomes Cultured in Vitro Chapter 21 Cultivation of Salivarian Trypanosomes: Application
- No. of pages:
- © Academic Press 1979
- 28th January 1979
- Academic Press
- eBook ISBN:
Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.
Rutgers University, New Brunswick, NJ, USA