Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source.
This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants.
This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Preface Acknowledgments Scheduling and Interrelationships of Exercises 1 Setup of a Tissue Culture Laboratory Work Areas 2 Media Components and Preparation Inorganic Salts Plant Growth Regulators Vitamins Carbohydrates Hexitols Gelling Agent Amino Acids Antibiotics Natural Complexes 3 Explant Preparation Aseptic Technique Aseptic Germination of Seeds 4 Callus Induction Callus Initiation Explant Orientation Establishment of Competent Cereal Cell Cultures (Rice) Salt Selection in Vitro Growth Curves Cellular Variation from Callus Cultures 5 Regeneration and Morphogenesis Controlled Morphogenesis: Potato Tuberization Somatic Embryogenesis Regeneration of Rice Dormancy Requirements of Explants Primary Morphogenesis: Douglas Fir 6 Haploid Plants from Anther Culture Datura African Violet Tobacco Anther Squash Technique 7 Embryo Rescue Sweet Corn Crabapple and Pear 8 Meristem Culture for Virus-Free Plants Isolation of Shoot Apical Meristem Establishing Virus- and Bacteria-Free Plants Garlic Propagation from Bulb Scales 9 In Vitro Propagation for Commercial Production of Ornamentals Boston Fern Staghorn Fern: Stages I, II, and III Ficus: Stages I, II, and III Kalanchoe Rose African Violet Endangered Species 10 Protoplast Isolation and Fusion 11 Agrobacterium-Mediated Transformation of Plants Petunia Leaf Disk Petunia Shoot Apex 12 Useful Measurements 13 Review of Solution Preparation Percentage Solutions Molar Solutions Dilution of a Stock Solution 14 List of Suppliers 15 Common Plant Tissue Culture Terms Index
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- © Academic Press 1992
- 13th July 1992
- Academic Press
- eBook ISBN: