Physical Principles and Techniques of Protein Chemistry Part B - 1st Edition - ISBN: 9780124401020, 9780323141130

Physical Principles and Techniques of Protein Chemistry Part B

1st Edition

Editors: Sydney Leach
eBook ISBN: 9780323141130
Imprint: Academic Press
Published Date: 1st January 1970
Page Count: 508
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Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

Table of Contents

List of Contributors


Contents of Other parts

10. Ultracentrifugal Analysis

Glossary of Symbols

I. Introduction

II. Fundamentals of the Method

III. Subsidiary Measurements Required in Ultracentrifugal Analysis

IV. Sedimentation Velocity Behavior of Noninteracting Solutes

V. Sedimentation Equilibrium of Noninteracting Solutes

VI. The Archibald Method. Equilibrium at the Menisci Only

VII. Sedimentation Behavior of Interacting Solutes

VIII. Zonal Analytical Velocity Sedimentation

IX. Isopycnic Density Gradient Sedimentation

X. A Brief Comparison of Some Commercially Available Ultracentrifuges


11. Viscosity

Glossary of Symbols

I. Introduction

II. Viscosity and Intrinsic Viscosity

III. Experimental Methods

IV. Theories for "Model" Particles of Various Shapes

V. Dependence of Viscosity or Intrinsic Viscosity on Various Factors

VI. Use of Viscosity Measurements in Protein Chemistry


12. Light Scattering

Glossary of Symbols

I. Introduction

II. Principles

III. Practice



13. Infrared Methods

Glossary of Symbols

I. Introduction

II. Measurement of Spectra

III. Quantitative Analysis of Spectra

IV. Correlation of Spectra with Structure


14. Nuclear Magnetic Resonance Spectroscopy

Glossary of Symbols

I. Introduction

II. Basic Theory of Nuclear Magnetic Resonance

III. Experimental Techniques

IV. Applications


15. Binding of Protons and Other Ions

Glossary of Symbols

I. Introduction

II. General Considerations in Ion Binding

III. Specificity of Binding Environments Illustrated for Hydrogen Ions

IV. Ion Binding in the Crystalline State

V. Ion Binding by Native Proteins in Solution

VI. Major Conformational Changes Related to Ion Binding

VII. Some Problems for Future Study


16. Differential Thermal Analysis

I. Introduction

II. The Basic Principles

III. Presentation of Data

IV. Instrumentation and Methodology

V. Limitations

VI. Applications to Polymers

VII. Protein Analyses

VIII. Future Applications

IX. Complementary Methods


Author Index

Subject Index


No. of pages:
© Academic Press 1970
Academic Press
eBook ISBN:

About the Editor

Sydney Leach

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