Physical Principles and Techniques of Protein Chemistry Part B

Physical Principles and Techniques of Protein Chemistry Part B

1st Edition - January 1, 1970

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  • Editor: Sydney Leach
  • eBook ISBN: 9780323141130

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Description

Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

Table of Contents


  • List of Contributors

    Preface

    Contents of Other parts

    10. Ultracentrifugal Analysis

    Glossary of Symbols

    I. Introduction

    II. Fundamentals of the Method

    III. Subsidiary Measurements Required in Ultracentrifugal Analysis

    IV. Sedimentation Velocity Behavior of Noninteracting Solutes

    V. Sedimentation Equilibrium of Noninteracting Solutes

    VI. The Archibald Method. Equilibrium at the Menisci Only

    VII. Sedimentation Behavior of Interacting Solutes

    VIII. Zonal Analytical Velocity Sedimentation

    IX. Isopycnic Density Gradient Sedimentation

    X. A Brief Comparison of Some Commercially Available Ultracentrifuges

    References

    11. Viscosity

    Glossary of Symbols

    I. Introduction

    II. Viscosity and Intrinsic Viscosity

    III. Experimental Methods

    IV. Theories for "Model" Particles of Various Shapes

    V. Dependence of Viscosity or Intrinsic Viscosity on Various Factors

    VI. Use of Viscosity Measurements in Protein Chemistry

    References

    12. Light Scattering

    Glossary of Symbols

    I. Introduction

    II. Principles

    III. Practice

    Appendix

    References

    13. Infrared Methods

    Glossary of Symbols

    I. Introduction

    II. Measurement of Spectra

    III. Quantitative Analysis of Spectra

    IV. Correlation of Spectra with Structure

    References

    14. Nuclear Magnetic Resonance Spectroscopy

    Glossary of Symbols

    I. Introduction

    II. Basic Theory of Nuclear Magnetic Resonance

    III. Experimental Techniques

    IV. Applications

    References

    15. Binding of Protons and Other Ions

    Glossary of Symbols

    I. Introduction

    II. General Considerations in Ion Binding

    III. Specificity of Binding Environments Illustrated for Hydrogen Ions

    IV. Ion Binding in the Crystalline State

    V. Ion Binding by Native Proteins in Solution

    VI. Major Conformational Changes Related to Ion Binding

    VII. Some Problems for Future Study

    References

    16. Differential Thermal Analysis

    I. Introduction

    II. The Basic Principles

    III. Presentation of Data

    IV. Instrumentation and Methodology

    V. Limitations

    VI. Applications to Polymers

    VII. Protein Analyses

    VIII. Future Applications

    IX. Complementary Methods

    References

    Author Index

    Subject Index


Product details

  • No. of pages: 508
  • Language: English
  • Copyright: © Academic Press 1970
  • Published: January 1, 1970
  • Imprint: Academic Press
  • eBook ISBN: 9780323141130

About the Editor

Sydney Leach

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