Phospholipases, Volume 197

1st Edition

Serial Volume Editors: Edward Dennis
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121820985
eBook ISBN: 9780080883120
Imprint: Academic Press
Published Date: 14th March 1991
Page Count: 640
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Institutional Access

Table of Contents

Phospholipase Assays, Kinetics, and Substrates:

L.J. Reynolds, W.N. Washburn, R.A. Deems, and E.A. Dennis, Assay Strategies and Methods for Phospholipases.

P. Elsbach and J. Weiss, Utilization of Labeled Escherichia coli as Phospholipase Substrate.

M.F. Roberts, Nuclear Magnetic Resonance Spectroscopy to Follow Phospholipase Kinetics and Products.

S. Ransac, H. Moreau, C. Rivi~abere, and R. Verger, Monolayer Techniques for Studying Phospholipase Kinetics.

L. Yu and E.A. Dennis, Thio-Based Phospholipase Assay.

W. Cho and F.J. K~aaezdy, Chromogenic Substrates and Assay of Phospholipases A2.

M.S. Jurkowitz-Alexander, Y. Hirashima, and L.A. Horrocks, Coupled Enzyme Assays for Phospholipase Activities with Plasmalogen Substrates.

H.S. Hendrickson, Phospholipase A2 Assays with Fluorophore-Labeled Lipid Substrates.

M.F. Roberts, Assays of Phospholipases on Short-Chain Phospholipids.

M.K. Jain and M.H. Gelb, Phospholipase A2-Catalyzed Hydrolysis of Vesicles: Uses of Interfacial Catalyzed Hydrolysis of Vesicles.

Uses of Interfacial Catalysis in Scooting Mode:

K.Y. Hostetler, M.F. Gardner, and K.A. Aldern, Assay of Phospholipases C and D in Presence of Other Lipid Hydrolases.

F. Paltauf and A. Hermetter, Preparation of Alky Ether and Vinyl Ether Substrates for Phospholipases.

M.R. Hanley, D.R. Poyner, and P.T. Hawkins, Inositol Phospholipids and Phosphates for Investigation of Intact Cell Phospholipase C Substrates and Products.

M.L. Blank and F. Snyder, Chromatographic Analysis of Phospholipase Reaction Products.

F.H. Chilton, Assays for Measuring Release of Arachidonic Acid from Phospholipids.<B


This volume on Phospholipases, the first of its kind, presents techniques for the assay, purification, and characterization of these enzymes, which are not only degradative enzymes of lipid metabolism but are also key enzymes in the signal transduction pathway.


Biochemists, molecular biologists, microbiologists, researchers in the pharmaceutical industry, biophysicists, and organic chemists.


No. of pages:
© Academic Press 1991
Academic Press
eBook ISBN:
Hardcover ISBN:


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About the Serial Volume Editors

Edward Dennis Serial Volume Editor

Edward A. Dennis: University of California, San Diego Distinguished Professor of Chemistry and Biochemistry and former Department Chair and Professor of Pharmacology in the School of Medicine, Dennis is currently Editor-in-Chief of the Journal of Lipid Research and Director of the LIPID MAPS initiative. He also served as Chair of the Board of Directors of the Keystone Symposia on Molecular and Cellular Biology and current as an Emeritus member of the Board of Directors. His current research focus is on phospholipases, cell signaling and lipidomics on which he has published over 300 papers, most related to signal transduction by lipid messengers

Affiliations and Expertise

University of California at San Diego, La Jolla, CA, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA