Description

This volume on Phospholipases, the first of its kind, presents techniques for the assay, purification, and characterization of these enzymes, which are not only degradative enzymes of lipid metabolism but are also key enzymes in the signal transduction pathway.

Readership

Biochemists, molecular biologists, microbiologists, researchers in the pharmaceutical industry, biophysicists, and organic chemists.

Table of Contents

Phospholipase Assays, Kinetics, and Substrates: L.J. Reynolds, W.N. Washburn, R.A. Deems, and E.A. Dennis, Assay Strategies and Methods for Phospholipases. P. Elsbach and J. Weiss, Utilization of Labeled Escherichia coli as Phospholipase Substrate. M.F. Roberts, Nuclear Magnetic Resonance Spectroscopy to Follow Phospholipase Kinetics and Products. S. Ransac, H. Moreau, C. Rivi~abere, and R. Verger, Monolayer Techniques for Studying Phospholipase Kinetics. L. Yu and E.A. Dennis, Thio-Based Phospholipase Assay. W. Cho and F.J. K~aaezdy, Chromogenic Substrates and Assay of Phospholipases A2. M.S. Jurkowitz-Alexander, Y. Hirashima, and L.A. Horrocks, Coupled Enzyme Assays for Phospholipase Activities with Plasmalogen Substrates. H.S. Hendrickson, Phospholipase A2 Assays with Fluorophore-Labeled Lipid Substrates. M.F. Roberts, Assays of Phospholipases on Short-Chain Phospholipids. M.K. Jain and M.H. Gelb, Phospholipase A2-Catalyzed Hydrolysis of Vesicles: Uses of Interfacial Catalyzed Hydrolysis of Vesicles. Uses of Interfacial Catalysis in Scooting Mode: K.Y. Hostetler, M.F. Gardner, and K.A. Aldern, Assay of Phospholipases C and D in Presence of Other Lipid Hydrolases. F. Paltauf and A. Hermetter, Preparation of Alky Ether and Vinyl Ether Substrates for Phospholipases. M.R. Hanley, D.R. Poyner, and P.T. Hawkins, Inositol Phospholipids and Phosphates for Investigation of Intact Cell Phospholipase C Substrates and Products. M.L. Blank and F. Snyder, Chromatographic Analysis of Phospholipase Reaction Products. F.H. Chilton, Assays for Measuring Release of Arachidonic Acid from Phospholipids.

Details

No. of pages:
640
Language:
English
Copyright:
© 1991
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883120
Print ISBN:
9780121820985

About the serial-volume-editor

Edward Dennis

Edward A. Dennis: University of California, San Diego Distinguished Professor of Chemistry and Biochemistry and former Department Chair and Professor of Pharmacology in the School of Medicine, Dennis is currently Editor-in-Chief of the Journal of Lipid Research and Director of the LIPID MAPS initiative. He also served as Chair of the Board of Directors of the Keystone Symposia on Molecular and Cellular Biology and current as an Emeritus member of the Board of Directors. His current research focus is on phospholipases, cell signaling and lipidomics on which he has published over 300 papers, most related to signal transduction by lipid messengers

Affiliations and Expertise

University of California at San Diego, La Jolla, CA, U.S.A.

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY