This volume on Phospholipases, the first of its kind, presents techniques for the assay, purification, and characterization of these enzymes, which are not only degradative enzymes of lipid metabolism but are also key enzymes in the signal transduction pathway.


Biochemists, molecular biologists, microbiologists, researchers in the pharmaceutical industry, biophysicists, and organic chemists.

Table of Contents

Phospholipase Assays, Kinetics, and Substrates: L.J. Reynolds, W.N. Washburn, R.A. Deems, and E.A. Dennis, Assay Strategies and Methods for Phospholipases. P. Elsbach and J. Weiss, Utilization of Labeled Escherichia coli as Phospholipase Substrate. M.F. Roberts, Nuclear Magnetic Resonance Spectroscopy to Follow Phospholipase Kinetics and Products. S. Ransac, H. Moreau, C. Rivi~abere, and R. Verger, Monolayer Techniques for Studying Phospholipase Kinetics. L. Yu and E.A. Dennis, Thio-Based Phospholipase Assay. W. Cho and F.J. K~aaezdy, Chromogenic Substrates and Assay of Phospholipases A2. M.S. Jurkowitz-Alexander, Y. Hirashima, and L.A. Horrocks, Coupled Enzyme Assays for Phospholipase Activities with Plasmalogen Substrates. H.S. Hendrickson, Phospholipase A2 Assays with Fluorophore-Labeled Lipid Substrates. M.F. Roberts, Assays of Phospholipases on Short-Chain Phospholipids. M.K. Jain and M.H. Gelb, Phospholipase A2-Catalyzed Hydrolysis of Vesicles: Uses of Interfacial Catalyzed Hydrolysis of Vesicles. Uses of Interfacial Catalysis in Scooting Mode: K.Y. Hostetler, M.F. Gardner, and K.A. Aldern, Assay of Phospholipases C and D in Presence of Other Lipid Hydrolases. F. Paltauf and A. Hermetter, Preparation of Alky Ether and Vinyl Ether Substrates for Phospholipases. M.R. Hanley, D.R. Poyner, and P.T. Hawkins, Inositol Phospholipids and Phosphates for Investigation of Intact Cell Phospholipase C Substrates and Products. M.L. Blank and F. Snyder, Chromatographic Analysis of Phospholipase Reaction Products. F.H. Chilton, Assays for Measuring Release of Arachidonic Acid from Phospholipids.


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© 1991
Academic Press
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