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Direct PCR on Clinical Specimens: M. Panaccio, M. French, and A. Lew, Formamide Low-Temperature PCR: Applications for Direct PCR from Clinical Material. B.-Y. Nordveg, H.M.F. Riise, G. Husby, I. Nilsen, and M.R.El-Gewely, Direct Use of Blood in PCR. Application of Reverse Transcription-Mediated PCR: T.K. Chatterjee, G. Sarkar, M.E. Bolander, and R.A. Fisher, Use of PCR for Isolation of Neuropeptide Receptor Genes. N.D. Borson, W.L. Salo, and L.R. Drewes, Lock-Docking Rapid Amplification of cDNA Ends PCR: Strategies and Applications. B.L. Ziegler, C.P. Lamping, S.J. Thoma, C.A. Thomas, and T.M. Fliedner, Single-Cell cDNA-PCR. Gene Synthesis by PCR: A. Darveau, A. Pelletier, and J. Perreault, PCR-Mediated Synthesis of Chimeric Molecules. K. Jayaraman, PCR-Mediated Gene Synthesis. Quantitative PCR: N.W. Soong and N. Arnheim, Quantitative PCR: Analysis of Rare Mitochondrial DNA Mutations in Central Nervous System Tissues. M. Becker-Andro, PCR-Aided Transcript Titration Assay: Competitive PCR for Evaluation of Absolute Levels of Rare mRNA Species. S. Sur, G.J. Gleich, M.E. Bolander, and G. Sarkar, Comparative Evaluation of Quantitative PCR Methods. Application of PCR in Mutation Detection: N. Bardeesy and J. Pelletier, Mutational Detection by Single-Strand Conformational Polymorphism. A. Sanyal and G.S. Getz, Detection of Mutation in Yeast hsp60 Gene by PCR. D. Glavac[breve] and M. Dean, Comparison of the Sensitivity of Single-Strand Conformational Polymorphism and Heteroduplex Methods. T.M. Cheng, V. Ganju, S.R. Ritland, G. Sarkar, and R.B. Jenkins, Analysis of p53 Mutations in Human Gliomas by RNA Single-Strand Conformational Polymorphism. Generation of Probes by PCR: H. Yang and P.W. Melera, PCR and Generation of Antisense RNA Probes for Use in RNase Protection Assays. S. Leonard, In Vitro Transcription of cRNA from PCR-Generated DNA Fragments. PCR in the Context of Cloning and Constructing Libraries: D. Kovalic and B. Weisblum, Direct Cloning of DNA Fragments Generated by PCR. E. Hara, Y. Furuichi, and K. Oda, Subtractive cDNA Cloning Using Oligo(dT)30-Latex and PCR. K.S. Miller and M. Brudnak, Expression Cloning: PCR versus Episomal Vectors for Rescue of Transfected Genes. R.P. Kandpal and S.M. Weissman, Use of PCR for Constructing Jumping Libraries. Site-Directed Mutagenesis by PCR: S. Barik, Site-Directed Mutagenesis by PCR: Substitution, Insertion, Deletion, and Gene Fusion. M.V. Kumar and D.J. Tindall, Use of PCR in Analysis of 5-Flanking Region of Androgen Receptor Gene. Application of PCR in AIDS Research: O. Bagasra and R.J.Pomerantz, Detection of HIV-1 in Brain Tissue of Individuals with AIDS by in Situ Gene Amplification. D.K. Dube, S. Dube, M.P. Sherman, J. Love, N.K. Saksena, W.J. Harrington, Jr., J.F. Ferrer, L. Papsidero, L. Dyster, R. Yanagihara, A.E. Williams, J.B. Glaser, V.M.A. Herve, F. Barre-Sinoussi, B.S. Blumberg, and B.J. Poiesz, Estimation of Genetic Heterogeneity in Primate T-Cell Lymphoma/Leukemia Viruses by PCR. S. Dube, B. Paul, V. Bryz-Gornia, C. Stephens, S. Erensoy, D.K. Dube, and B.J. Poiesz, Use of PCR in Detection of Antisense Transcripts in HTLV-I-Infected Patients and Human T-Cell Lines. Miscellaneous Uses of PCR: A.M. Douglas and B.A. Atchison, Direct Chemiluminescent Sequencing of Double-Stranded PCR Products. A. Christoph and H.-J. Thiesen, Use of PCR to Determine Genomic DNA Target Sites for Zinc Finger Protein Expressed in Mouse Cerebellum. R.A. Robinson and M.J. Heller, DNA Extraction from Archived Specimens by Sonication. Index.
The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike.
- Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue)
- Complete retrieval of novel expressed genes by PCR without screening a library
- Quantitation by PCR
- Mutagenesis by PCR
- PCR in AIDS research
- Simple and effective protocols for PCR on archival specimens
Neuroscientists, microbiologists, geneticists, molecular biologists, biochemists, graduate students and teachers in these disciplines, and AIDS researchers.
- No. of pages:
- © Academic Press 1995
- 3rd March 1995
- Academic Press
- eBook ISBN:
"Well-written, clear and detailed... The authors do indeed offer the 'fine detail, tricks and short cuts not usually found in the written word." --TRENDS IN NEUROSCIENCES
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.
Texas Tech University Health Sciences Center, Lubbock, USA
Mayo Clinic, Rochester, Minnesota, U.S.A.
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