Total Antioxidant Activity:
H. Alho and J. Leinonen, Total Antioxidant Activity Measured by Chemiluminescence Methods.
I.F.F. Benzie and J.J. Strain, Ferric Reducing/Antioxidant Power Assay: Direct Measure of "Total Antioxidant Activity" of Biological Fluids, and a Modified Version (FRASC) Which Enables the Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration.
Y. Noda, M. Kohno, A. Mori, and L. Packer, Automated ESR Free Radical Detector Assays for Antioxidant Activity in Natural Extracts.
A. Kontush and U. Beisiegel, Measurement of Oxidizability of Blood Plasma.
G. Cao and R.L. Prior, Measurement of Oxygen Radical Absorbance Capacity in Biological Samples.
M. Levine, Y. Wang, and S.C. Rumsey, Analysis of Ascorbic and Dehydroascorbic Acid in Biological Samples.
A.M. Bode and R.C. Rose, Analysis of Water-Soluble Antioxidants by High-Performance Liquid Chromatography with Electrochemical Detection.
J. Lykkesfeldt and B.N. Ames, Ascorbic Acid Recycling in Rat Hepatocytes as a Measurement of Antioxidant Capacity: Decline with Age.
Polyphenols and Flavonoids:
L.C. Bourne and C. Rice-Evans, Method for Detecting and Measuring Bioavailability of Phenolics and Flavonoids in Humans: Pharmacokinetics of Urinary Excretion of Dietary Ferulic Acid.
T. Goto and Y. Yoshida, Simultaneous Analysis of Individual Catechins and Caffeine in Green Tea.
A.L. Waterhouse, S.F. Price, and J.D. McCord, Reverse Phase HPLC Methods for Analysis of Wine Polyphenols.
D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by High-Performance Liquid Chromatography.
D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by Gas Chromatography-Mass Spectrometry.
V.L. Singleton, R. Orthofer, and R.M. Lamuela-Raventós, Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteau Reagent.
V. Cheynier, J.-M. Souquet, E. LeRoux, S. Guyot, and J. Riguad, Size Separation of Condensed Tannins by Normal-Phase HPLC.
R.M. Lamuela-Raventós and A. Waterhouse, Resveratrol and Piceid in Wine.
E.N. Frankel, Antioxidant Activity by Headspace Gas Chromatography of Volatile Oxidation Products of w-6 and w-3 p Polyunsaturated Lipids.
P.C.H. Hollman, D.P. Venema, S. Khokhar, and I.C.W. Arts, Determination of Tea Catechins by Reversed Phase HPLC.
A.S. Pannala, S. Singh, and C. Rice-Evans, Flavonoids as Peroxynitrite Scavengers in Vitro.
C.K. Sen, S. Roy, S. Khanna, and L. Packer, Determination of Oxidized and Reduced Lipoic Acid Using High Performance Liquid Chromatography and Coulometric Detection.
C.K. Sen, S. Roy, and L. Packer, Flow Cytometric Determination of Cellular Thiols.
L.A. Ridnour, R.A. Winters, N. Ercal, and D.R. Spitz, Measurement of Glutathione, Glutathione Disulfide and Other Thiols in Mammalian Cell and Tissue Homogenates Using HPLC Separation of N-(1n-pyrenyl)maleimide Derivatives.
M. Asensi, J. Sastre, F.V. Pallardo, A. Lloret, M. Lehner, J. Garcia-De-La Asuncion, and J. Viña, GSH:GSSG Ratio as an Indicator of Oxidative Stress Status and DNA Damage.
J. Chaudiere, N. Aguini, and J.-C. Yadan, Nonenzymatic Colorimetric Assay of Glutathione in the Presence of Other Mercaptans.
H. Baker, B. DeAngelis, E.R. Baker, and S.H. Hutner, Protozoological Method for Assaying Lipoate in Human Biologic Fluids and Tissue.
O. Tirosh, Y. Katzhendler, Y. Barenholz, and R. Kohen, Antioxidant Activity of Amidothionophosphates.
K. Traber and L. Packer, Quantitation of Anethole Dithiolthione Using High Pressure Liquid Chromatography with Electrochemical Detection. Vitamin E and Coenzyme Q10:
D.C. Liebler, J.A. Burr, and A.J.L. Ham, Gas Chromatography-Mass Spectrometry Analysis of Vitamin E and Its Oxidation Products.
J.K.G. Kramer, R.C. Fouchard, and K.M.R. Kallury, Improved Method to Determine Vitamin E Forms in Tissues and Diets by High Performance Liquid Chromatography Using a Normal-Phase Diol Column.
M. Podda, C. Weber, M.G. Traber, R. Milbradt, and L. Packer, Sensitive HPLC Techniques for Simultaneous Determination of Tocopherols, Tocotrienols, Ubiquinols, and Ubiquinones in Biological Samples.
B. Finckh, A. Kontush, J. Commentz, C. Hübner, M. Burdelski, and A. Kohlschütter, HPLC Analysis of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma--Coulometric Electrochemical Detection.
C.-P. Aebischer, J. Schierle, and W. Schüep, Simultaneous Determination of Retinol, Tocopherols, Carotene Lycopene and Xanthophylls in Plasma by Means of Reversed Phase HPLC.
P.K. Witting, D. Mohr, and R. Stocker, Assessment of the Prooxidant Activity of Vitamin E in Human Low Density Lipoprotein and Plasma.
Carotenoids and Retinoids:
N. Pellegrini, R. Re, M. Yang, and C.A. Rice-Evans, Screening of Dietary Carotenoids and Carotenoid-Rich Fruit Extracts for Antioxidant Activities Applying the ABTs Radical Cation Decolorization Assay.
T. Wingerath, D. Kirsch, R. Kaufmann, W. Stahl, and H. Sies, Matrix-Assisted Laser Desorption Ionization Postsource Decay Mass Spectrometry (MALDI-PSD-MS) in Carotenoid Analysis.
A. Mortensen and L.H. Skibsted, Carotenoid Photobleaching.
M.A. Livrea and L. Tesoriere, Interactions Between Vitamin A and Vitamin E in Liposomes and in Biological Contexts.
T.E. Gundersen and R. Blomhoff, On-Line Solid Phase Extraction and Isocratic Separation of Retinoic Acid Isomers in a Micro-bore Column Switching System.
M.R. Lakshman and M.N. Rao, Purification and Characterization of Cellular Carotenoid-Binding Protein from Mammalian Liver.
J.P. Landrum, R.A. Bone, L.L. Moore, and C.M. Gomez, Analysis of Zeaxanthin Stereoisomer Distribution within Individual Human Retinas. Subject Index.
General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
@introbul:Key Features @bul:* Total Antioxidant Activity
- Vitamin C
- Polyphenols and Flavanoids
- Vitamin E and Coenzyme Q10
- Carotenoids and Retinoids
AUDIENCE: Researchers in biochemistry, pharmacology, physiology, cell biology, molecular biology, and biomedicine.
- No. of pages:
- © Academic Press 1999
- 2nd October 1998
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA
Helmut Sies is an Honorary Member of the American Society for Biochemistry and Molecular Biology. He received an Honorary Ph.D. from the University of Buenos Aires, Argentina in 1996. Dr. Sies is a member of the Northrhine-Westphalian Academy of Sciences, Germany, and a Corresponding Member of both the Academy of Sciences of Heidelberg, Germany, and the Academy of Medicine, Buenos Aires, Argentina. He has received many awards and prizes, including the FEBS Anniversary Prize awarded by the Federation of European Biochemical Societies, 1978; the Distinguished Foreign Scholar award, MASUA, 1985; the Silver Medal, Karolinska Institute, Stockholm, 1986; the Ernst Jung Preis fur Medizin, 1988; the Claudius-Galenus-Preis, 1990; and the ISFE-Preis, 1994. Dr. Sies sereves on the editorial board and advisory committee for twelve journals, has edited numerous books, and has published more than 400 original articles and chapters. He received his M.D. at the University of Munich in 1967 and currently serves as Full Professor and Chairman of the Department of Physiological Chemistry at the University of Düsseldorf.
Institut Fur Physiologische Chemie, Germany