Organ Culture
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Organ Culture provides information pertinent to the fundamental aspects of the study of autodifferentiation. This book discusses the physiological differentiation, the action of substances that inhibit or stimulate growth, and the interactions of associated organs. Organized into 10 chapters, this book begins with an overview of the differentiation of embryonic organs in natural media. This text then presents the study of differentiation in synthetic solid or liquid media and explains the biochemical and physiological requirements of the tissues. Other chapters consider the problems of sexual differentiation and intersexuality in vitro by providing a comparative study of avian and mammalian embryos. This book analyzes as well the interactions between hormones, inhibitor substances, and receptor organs in vitro. The final chapter deals with the study of organ chimeras in vitro and the related study of the organ culture of malignant tumors. This book is a valuable resource for embryologists and research workers.
Table of Contents
List of Contributors
Preface
Addendum
Chapter I. The General Principles of Organ Culture in Vitro
I. Explanation in Vivo and in Vitro
II. Conclusions
Bibliography
Chapter II. Differentiation of Organs in Natural Media
I. Examples of Differentiation in Embryonic Organs
II. Analysis of the Characteristics of Differentiation in Vitro
Bibliography
Addendum
Chapter III. Culture of Embryo Organs in Synthetic Media
I. Introduction
II. Methods for the Culture of Organs in Synthetic Media
III. Culture of the Gonads
IV. Culture of the Syrinx
V. Culture of the Skin
VI. Thyroid Culture
VII. Culture of the Tibiotarsus
VIII. Conclusions
Bibliography
Addendum
Chapter IV. Sexual Differentiation and Intersexuality in Vitro
I. Introduction
II. Birds
III. Mammals
IV. Amphibians
Bibliography
Addendum
Chapter V. The Action of Hormones and Inhibitors on Organs Cultured in Vitro
I. Interactions in Vitro Between Endocrine Glands and Target Organs in Parabiosis
II. Effects of Hormones and Inhibitor Substances Added to the Culture Medium
III. General Conclusions
Bibliography
Addendum
Chapter VI. Culture and Parabiosis of Blastoderms
I. Introduction
II. The Culture of Avian Blastoderms
III. In Vitro Culture of Mammalian Ova
IV. Conclusions
Bibliography
Addendum
Chapter VII. Organ Culture in the Invertebrates
I. Introduction
II. Experimental Techniques for the Survival and Function of Various Organs in Vitro
III. The Use of Culture Techniques for Research into Sexuality
IV. Organ Culture Applied to Endocrinological Research in Embryogenesis and Genetics
V. Organ Culture Applied to Experiments on Regeneration
VI. Conclusions
Bibliography
Addendum
Chapter VIII. In Vitro Reassociation of Dissociated Cells
I. Introduction
II. Morphological Aspects of Reaggregation
III. Interpretations of the Phenomenon of Adhesion
IV. Conclusion
Bibliography
Addendum
Chapter IX. Study of Organogenesis by Dissociation and Reassociation of Embryonic Rudiments in Vitro
I. Introduction
II. Analysis of Primary Induction
III. Analysis of the Induction of Vertebral Cartilage
IV. Analysis of the Morphogenic Interactions Between Epithelium and Mesenchyme in the Rudiments of Heterogeneous Organs
V. Conclusions
Bibliography
Addendum
Chapter X. Organ Chimeras and Organ Culture of Malignant Tumors
I. Introduction
II. Organ Chimeras
III. The Problem of Heteroplastic Associations Between Embryonic Avian Organs, Mammalian Malignant Tumors, and Human Malignant Tumors
IV. Conclusions
V. General Conclusions
Bibliography
Addendum
Subject Index
Product details
- No. of pages: 526
- Language: English
- Copyright: © Academic Press 1970
- Published: January 1, 1970
- Imprint: Academic Press
- eBook ISBN: 9780323147118