Organ Culture

1st Edition - January 1, 1970
Editor: J Thomas
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 4 7 1 1 - 8

Organ Culture provides information pertinent to the fundamental aspects of the study of autodifferentiation. This book discusses the physiological differentiation, the action of… Read more

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Organ Culture provides information pertinent to the fundamental aspects of the study of autodifferentiation. This book discusses the physiological differentiation, the action of substances that inhibit or stimulate growth, and the interactions of associated organs. Organized into 10 chapters, this book begins with an overview of the differentiation of embryonic organs in natural media. This text then presents the study of differentiation in synthetic solid or liquid media and explains the biochemical and physiological requirements of the tissues. Other chapters consider the problems of sexual differentiation and intersexuality in vitro by providing a comparative study of avian and mammalian embryos. This book analyzes as well the interactions between hormones, inhibitor substances, and receptor organs in vitro. The final chapter deals with the study of organ chimeras in vitro and the related study of the organ culture of malignant tumors. This book is a valuable resource for embryologists and research workers.

List of Contributors

Preface

Addendum

Chapter I. The General Principles of Organ Culture in Vitro

I. Explanation in Vivo and in Vitro

II. Conclusions

Bibliography

Chapter II. Differentiation of Organs in Natural Media

I. Examples of Differentiation in Embryonic Organs

II. Analysis of the Characteristics of Differentiation in Vitro

Bibliography

Addendum

Chapter III. Culture of Embryo Organs in Synthetic Media

I. Introduction

II. Methods for the Culture of Organs in Synthetic Media

III. Culture of the Gonads

IV. Culture of the Syrinx

V. Culture of the Skin

VI. Thyroid Culture

VII. Culture of the Tibiotarsus

VIII. Conclusions

Bibliography

Addendum

Chapter IV. Sexual Differentiation and Intersexuality in Vitro

I. Introduction

II. Birds

III. Mammals

IV. Amphibians

Bibliography

Addendum

Chapter V. The Action of Hormones and Inhibitors on Organs Cultured in Vitro

I. Interactions in Vitro Between Endocrine Glands and Target Organs in Parabiosis

II. Effects of Hormones and Inhibitor Substances Added to the Culture Medium

III. General Conclusions

Bibliography

Addendum

Chapter VI. Culture and Parabiosis of Blastoderms

I. Introduction

II. The Culture of Avian Blastoderms

III. In Vitro Culture of Mammalian Ova

IV. Conclusions

Bibliography

Addendum

Chapter VII. Organ Culture in the Invertebrates

I. Introduction

II. Experimental Techniques for the Survival and Function of Various Organs in Vitro

III. The Use of Culture Techniques for Research into Sexuality

IV. Organ Culture Applied to Endocrinological Research in Embryogenesis and Genetics

V. Organ Culture Applied to Experiments on Regeneration

VI. Conclusions

Bibliography

Addendum

Chapter VIII. In Vitro Reassociation of Dissociated Cells

I. Introduction

II. Morphological Aspects of Reaggregation

III. Interpretations of the Phenomenon of Adhesion

IV. Conclusion

Bibliography

Addendum

Chapter IX. Study of Organogenesis by Dissociation and Reassociation of Embryonic Rudiments in Vitro

I. Introduction

II. Analysis of Primary Induction

III. Analysis of the Induction of Vertebral Cartilage

IV. Analysis of the Morphogenic Interactions Between Epithelium and Mesenchyme in the Rudiments of Heterogeneous Organs

V. Conclusions

Bibliography

Addendum

Chapter X. Organ Chimeras and Organ Culture of Malignant Tumors

I. Introduction

II. Organ Chimeras

III. The Problem of Heteroplastic Associations Between Embryonic Avian Organs, Mammalian Malignant Tumors, and Human Malignant Tumors

IV. Conclusions

V. General Conclusions

Bibliography

Addendum

Subject Index