Optical Techniques

Optical Techniques

Physical Techniques in Biological Research

2nd Edition - January 1, 1971

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  • Editor: Gerald Oster
  • eBook ISBN: 9781483191805

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Physical Techniques in Biological Research, Second Edition, Volume I, Part A: Optical Techniques focuses on the methods and theory applied to relatively pure preparations of biological substances that are derived from cells or other tissue elements. This book covers a wide variety of nonoptical techniques. Organized into five chapters, this edition begins with an overview of the fundamental principles of operation of optical instruments that can be explained in terms of the simple operations of lenses or light rays. This text then examines the limits imposed on direct observation, either through an optical instrument or with the unaided eye, by the nature of light itself. Other chapters consider the potential of certain other microprobe methods. This book discusses as well the process of molecular spectroscopy. The final chapter deals with optical rotation and its dependence on wavelength. This book is a valuable resource for biologists, scientists, physicists, physical chemists, and research workers.

Table of Contents

  • List of Contributors

    Preface to the Second Edition of Volume I

    Preface to the First Edition of Volume I

    Contents of Volume IB

    Chapter 1 The Light Microscope

    I. Principles of Lenses and Lens Systems

    II. Physical Theory

    III. The Eye in Relation to Microscopy

    IV. The Optical and Mechanical Systems of the Microscope

    V. Adjustment and Use of the Microscope

    VI. General Theory of Image Formation in the Microscope


    Chapter 2 Electron Microscopy

    I. The Limits of Optical Observation

    II. Basic Principles of Electron Microscopy

    III. Practical Differences between Optical and Electron Microscopy

    IV. Elements of Electron Lenses

    V. The Illuminating System

    VI. The Imaging System

    VII. Recording the Image

    VIII. Requirements for High Performance

    IX. Specimen Preparation

    X. Artifacts

    XI. The Scanning Electron Microscope

    XII. The High Voltage Electron Microscope

    XIII. Other Types of Electron Microscope


    Chapter 3 The Microprobe Assay of Chemical Elements

    I. Introduction

    II. Components and Modes of Operation of the Standard Microprobe

    III. Types of Instrumentation and Their Performance

    IV. Spatial Resolution of the X-Ray Analysis

    V. Sensitivity

    VI. Quantitation

    VII. Specimen Preparation

    VIII. Damage to Specimens in the Electron-Probe Instrument

    IX. Other Types of Microprobe Analysis


    Appendix I. Manufacturers and Availability of Probe Instruments

    Appendix II. Bibliography of Biological Applications of Electron-Probe X-Ray Microanalysis

    Chapter 4 Infrared and Raman Spectroscopy

    I. Introduction

    II. Infrared Methods

    III. Raman Methods


    Chapter 5 Optical Rotatory Dispersion and Circular Dichroism

    I. Introduction

    II. Polynucleotides, RNA and DNA

    III. Polypeptides and Proteins

    IV. Polysaccharides

    V. Concluding Remarks


    Author Index

    Subject Index

Product details

  • No. of pages: 444
  • Language: English
  • Copyright: © Academic Press 1971
  • Published: January 1, 1971
  • Imprint: Academic Press
  • eBook ISBN: 9781483191805

About the Editor

Gerald Oster

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