Optical Techniques - 2nd Edition - ISBN: 9780125296014, 9781483191805

Optical Techniques

2nd Edition

Physical Techniques in Biological Research

Editors: Gerald Oster
eBook ISBN: 9781483191805
Imprint: Academic Press
Published Date: 1st January 1971
Page Count: 444
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Physical Techniques in Biological Research, Second Edition, Volume I, Part A: Optical Techniques focuses on the methods and theory applied to relatively pure preparations of biological substances that are derived from cells or other tissue elements. This book covers a wide variety of nonoptical techniques. Organized into five chapters, this edition begins with an overview of the fundamental principles of operation of optical instruments that can be explained in terms of the simple operations of lenses or light rays. This text then examines the limits imposed on direct observation, either through an optical instrument or with the unaided eye, by the nature of light itself. Other chapters consider the potential of certain other microprobe methods. This book discusses as well the process of molecular spectroscopy. The final chapter deals with optical rotation and its dependence on wavelength. This book is a valuable resource for biologists, scientists, physicists, physical chemists, and research workers.

Table of Contents

List of Contributors Preface to the Second Edition of Volume I Preface to the First Edition of Volume I Contents of Volume IB Chapter 1 The Light Microscope I. Principles of Lenses and Lens Systems II. Physical Theory III. The Eye in Relation to Microscopy IV. The Optical and Mechanical Systems of the Microscope V. Adjustment and Use of the Microscope VI. General Theory of Image Formation in the Microscope References Chapter 2 Electron Microscopy I. The Limits of Optical Observation II. Basic Principles of Electron Microscopy III. Practical Differences between Optical and Electron Microscopy IV. Elements of Electron Lenses V. The Illuminating System VI. The Imaging System VII. Recording the Image VIII. Requirements for High Performance IX. Specimen Preparation X. Artifacts XI. The Scanning Electron Microscope XII. The High Voltage Electron Microscope XIII. Other Types of Electron Microscope References Chapter 3 The Microprobe Assay of Chemical Elements I. Introduction II. Components and Modes of Operation of the Standard Microprobe III. Types of Instrumentation and Their Performance IV. Spatial Resolution of the X-Ray Analysis V. Sensitivity VI. Quantitation VII. Specimen Preparation VIII. Damage to Specimens in the Electron-Probe Instrument IX. Other Types of Microprobe Analysis References Appendix I. Manufacturers and Availability of Probe Instruments Appendix II. Bibliography of Biological Applications of Electron-Probe X-Ray Microanalysis Chapter 4 Infrared and Raman Spectroscopy I. Introduction II. Infrared Methods III. Raman Methods References Chapter 5 Optical Rotatory Dispersion and Circular Dichroism I. Introduction II. Polynucleotides, RNA and DNA III. Polypeptides and Proteins IV. Polysaccha


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© Academic Press 1971
Academic Press
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About the Editor

Gerald Oster

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