Optical Techniques - 2nd Edition - ISBN: 9780125296014, 9781483191805

Optical Techniques

2nd Edition

Physical Techniques in Biological Research

Editors: Gerald Oster
eBook ISBN: 9781483191805
Imprint: Academic Press
Published Date: 1st January 1971
Page Count: 444
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Physical Techniques in Biological Research, Second Edition, Volume I, Part A: Optical Techniques focuses on the methods and theory applied to relatively pure preparations of biological substances that are derived from cells or other tissue elements. This book covers a wide variety of nonoptical techniques. Organized into five chapters, this edition begins with an overview of the fundamental principles of operation of optical instruments that can be explained in terms of the simple operations of lenses or light rays. This text then examines the limits imposed on direct observation, either through an optical instrument or with the unaided eye, by the nature of light itself. Other chapters consider the potential of certain other microprobe methods. This book discusses as well the process of molecular spectroscopy. The final chapter deals with optical rotation and its dependence on wavelength. This book is a valuable resource for biologists, scientists, physicists, physical chemists, and research workers.

Table of Contents

List of Contributors

Preface to the Second Edition of Volume I

Preface to the First Edition of Volume I

Contents of Volume IB

Chapter 1 The Light Microscope

I. Principles of Lenses and Lens Systems

II. Physical Theory

III. The Eye in Relation to Microscopy

IV. The Optical and Mechanical Systems of the Microscope

V. Adjustment and Use of the Microscope

VI. General Theory of Image Formation in the Microscope


Chapter 2 Electron Microscopy

I. The Limits of Optical Observation

II. Basic Principles of Electron Microscopy

III. Practical Differences between Optical and Electron Microscopy

IV. Elements of Electron Lenses

V. The Illuminating System

VI. The Imaging System

VII. Recording the Image

VIII. Requirements for High Performance

IX. Specimen Preparation

X. Artifacts

XI. The Scanning Electron Microscope

XII. The High Voltage Electron Microscope

XIII. Other Types of Electron Microscope


Chapter 3 The Microprobe Assay of Chemical Elements

I. Introduction

II. Components and Modes of Operation of the Standard Microprobe

III. Types of Instrumentation and Their Performance

IV. Spatial Resolution of the X-Ray Analysis

V. Sensitivity

VI. Quantitation

VII. Specimen Preparation

VIII. Damage to Specimens in the Electron-Probe Instrument

IX. Other Types of Microprobe Analysis


Appendix I. Manufacturers and Availability of Probe Instruments

Appendix II. Bibliography of Biological Applications of Electron-Probe X-Ray Microanalysis

Chapter 4 Infrared and Raman Spectroscopy

I. Introduction

II. Infrared Methods

III. Raman Methods


Chapter 5 Optical Rotatory Dispersion and Circular Dichroism

I. Introduction

II. Polynucleotides, RNA and DNA

III. Polypeptides and Proteins

IV. Polysaccharides

V. Concluding Remarks


Author Index

Subject Index


No. of pages:
© Academic Press 1971
Academic Press
eBook ISBN:

About the Editor

Gerald Oster

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