Description

This volume is a comprehensive guide to the methodologies used in the study of structural domains of cell nuclei. The text covers chromatin, the karyoskeleton, the soluble domain, and the nucleolus. It details methods that are used to isolate components from these domains and techniques used to assemble and disassemble nuclear elements. There is also coverage of three-dimensional mapping and localization of nuclear processes.

Key Features

@introbul:Key Features @bul:* Provides a practical laboratory guide for studying cell nuclei * Includes comprehensive and easy-to-follow protocols

Readership

Graduate students, technicians, postdoctoral, and experienced researchers. Cell, developmental, and molecular biologists, geneticists, human genome researchers, and others studying nuclear structure and organization.

Table of Contents

M. Berrios, Introduction. Cell Fractionation Preparation and Characterization of Probes: M. Berrios, Isolation and Characterization of Nuclear Pore Complex-Lamina Fractions from Vertebrates. P.A. Fisher,Preparation and Characterization of Karyoskeletal Protein-Enriched Fractions from Drosophila melanogaster. J.P. Aris, Isolation of Nuclei and Nucleoli from Yeast Saccharomyces cerevisiae. Structural Analysis of the Interphase Nucleus: M.R. Paddy, Qualitative and Quantitative Approaches to the Spatial Localization of Nuclear Proteins with the Fluorescence Light Microscope. U. Kubitscheck and R. Peters, Localization of Single Nuclear Pore Complexes by Confocal Laser Scanning Microscopy and Analysis of their Distribution. A.S. Belmont, Nuclear Ultrastructure Transmission Electron Microscopy and Image Analysis. T.D. Allen, S.A. Rutherford, G.R. Bennion, M.W. Goldberg, and E. Kiseleva, Three-Dimensional Surface Structure Analysis of the Nucleus. J. Hainfeld and J. Wall, Scanning Transmission Electron Microscopy of Nucleic Acid-Protein Complexes. Chromatin and Sub-Nuclear Structures: C.L. Woodcock and R.A. Horowitz, Chromatin and Nucleosomal Assemblies. A.F. Dernburg and J.W. Sedat, Mapping the Architecture of Interphase Nucleus. A.J. Solari, Structural Analysis of Meiotic Chromosomes and Synaptonemal Complexes in Higher Vertebrates. J. Engebrecht, F. Klein, and J. Loidl, Genetic and Morphological Approaches for the Analysis of Meiotic Chromosomes in Yeast. M. Grote and R. Peters, Mapping Proteins to Nuclear Pore Complexes by Immunogold Electron Microscopy. R.L

Details

No. of pages:
625
Language:
English
Copyright:
© 1997
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080859514
Print ISBN:
9780125641555

About the serial-volume-editor

Reviews

@qu:"Nuclear Structure and Function is remarkably up to date, and all of the authors have produced clear and very useful contributions. This handbook is highly recommended and will be essential for researchers investigating all aspects of the nucleus, from the biochemistry of specific processes through to nuclear architecture. It will also be very helpful to all those who want to use some of these techniques as part of their research, perhaps to investigate the function of a particular gene product or the control of its expression." @source:--TIBS