Nuclear Particles

The Cell Nucleus, Vol. 8

1st Edition - January 28, 1981
Editor: Harris Busch
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 1 6 8 0 - 5

The Cell Nucleus, Volume III: Nuclear Particles, Part A covers topics on "splicing" and "processing" and on the controls of transcriptional and transport events that must be… Read more

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The Cell Nucleus, Volume III: Nuclear Particles, Part A covers topics on "splicing" and "processing" and on the controls of transcriptional and transport events that must be essential to cells that are either growing and/or are phenotypically differentiated. The book discusses the immunolocalization and structural organization of nascent RNP; the in situ localization of RNA structures; and the morphogenesis, cytochemistry, and putative role of perichromatin granules. The text describes transcription in the isolated nuclei; the isolation and structure of the ribonucleoprotein fibrils containing heterogeneous nuclear RNA; and low-molecular-weight nuclear ribonucleoprotein particles. The sequence, function, and role of U snRNAs of nuclear snRNPs; the maturation of low-molecular-weight RNA species; and the properties of the heterogeneous nuclear RNA-protein complexes and nuclear matrix are also encompassed. The book further tackles human antibodies to RNA-containing particles. Cellular biologists, microbiologists, pharmacologists, geneticists, and students taking related courses will find the book useful.

Contributors

Preface

Contents of Other Volumes

Chapter 1 Immunolocalization and Structural Organization of Nascent RNP

I. Introduction

II. Transcriptional Activities

III. Interaction of Nascent RNA Transcripts with Proteins

IV Immunological Studies on Nascent RNP Transcripts

V Ultrastructural Analysis of Nascent RNP Transcripts

VI. Functional Activities of Nascent RNP Transcripts

References

Chapter 2 In Situ Localization of RNA Structures

I. Introduction

II. Methods in Ultrastructural Detection of RNA

III. General Organization of Nuclear RNA Structures

IV The Border of Condensed Chromatin

V. RNA Structures Present in the Interchromatin Space

VI. The Nucleolus

VII. Conclusions

References

Chapter 3 Perichromatin Granules

I. Introduction

II. Morphogenesis and Fine Structure of the PCG's

III. Variations in the Number of PCG's

IV. Cytochemistry of PCG's in Situ

V. Isolation of the PCG's

VI. Putative Role of the PCG's

References

Chapter 4 Transcription in Isolated Nuclei

I. Introduction

II. Techniques for Isolating Nuclei

III. Transcription in Isolated Nuclei

IV. Modification of Transcriptional Activity

V. Conclusions

References

Chapter 5 Isolation and Structure of the Ribonucleoprotein Fibrils Containing Heterogeneous Nuclear RNA

I. Introduction

II. Isolation of hnRNP

III. The Two Classes of Units of hnRNP

IV. The Constituents of hnRNP

V. Conclusions

References

Chapter 6 Low-Molecular-Weight Nuclear Ribonucleoprotein Particles

I. Introduction

II. Isolation and Characterization of Low-Molecular-Weight Nuclear RNP's Containing snRNA Species (snRNP's)

III. The Presence of snRNA in hnRNP Complexes

IV. Relation of the 10 S snRNP Particles to 30-50 S Structures

V. Poly(A)-Containing Low-Molecular-Weight Nuclear RNP Particles

VI. Biological Role of snRNP's

VII. Conclusions

References

Chapter 7 U snRNA's of Nuclear snRNP's

I. Introduction

II. Primary Sequence of snRNA's

III. Other Studies on snRNA's

IV. Functions of snRNA's

V. Structural Homologies of snRNA's

References

Chapter 8 Maturation of Low-Molecular-Weight RNA Species

I. Introduction

II. Maturation

References

Chapter 9 Heterogeneous Nuclear RNA- Protein Complexes and Nuclear Matrix

I. Introduction

II. Properties of hnRNA-Protein Complexes

III. Heterogeneous Nuclear RNA and Small-Molecular-Weight RNA

IV. Proteins in nRNP Complexes

V. nRNP Complexes and Nuclear Matrix

VI. Models

VII. Conclusion and Prospects

References

Chapter 10 Human Antibodies to RNA-Containing Particles

I. Introduction

II. Ribosomes

III. Nucleoli

IV. Nuclear RNP Particles

References

Index