Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich.
Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.
- Includes detailed and clearly-written step-by-step protocols
- Evinces expected results and offers trouble shooting advice
- Provides techniques appropriate for small laboratories as well as those with limited resources
- Covers a broad variety of cloning systems, including single copy vectors
- Discusses a diverse range of organisms, from prokaryotes to eukaryotes, from single-celled organisms to highly complex organisms
Ideal for advanced undergraduates, graduate students, postdocs, technicians and research directors in microbiology, genetics, molecular biology, plant and crop science, developmental biology, parasitology, cell biology, and other biological sciences.
J.S. Lee, B. Birren, and E. Lai, Introduction to Pulsed Field Gels, Preparation, and Analysis of Large DNA.
K. Dewar, L. Bernier, and R. Levesque, Electrophoretic Karyotyping in Fungi.
M. Ganal, Isolation and Analysis of High Molecular Weight DNA from Plants.
J.A. Rafalski, M. Morgante, W. Powell, J.M. Vogel, and S.V. Tingey, Generating and Using DNA Markers in Plants.
S. Morzaria, Genome Mapping of Protozoan Parasites by Linking Clones.
U. Rimling, R. Fislage, and B. Tummler, Mapping and Analysis of Bacterial Genomes.
R. Wenzal and R. Herrmann, Cosmid Cloning with Small Genomes.
C.T. Amemiya, T. Ota, and G.W. Litman, Construction of P1 Artificial Chromosome (PAC) Libraries from Lower Vertebrates.
S. Melville, N. Shepherd, C. Gerrard, and R. Le Page, The Selection of Chromosome-Specific DNA Clones from Africian Trypanosome Genomic Libraries.
A. Kuspa and W. Loomis, Analysis of the Dictyostelium discoideum Genome.
J.D. Hoheisel, E. Maier, R. Mott, and H. Lehrach, Integrated Genome Mapping by Hybridization Techniques. Subject Index.
- No. of pages:
- © Academic Press 1996
- 5th August 1996
- Academic Press
- eBook ISBN:
California Institute of Technology, Pasadena, U.S.A.
University of North Carolina, Chapel Hill, U.S.A.