Description

Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.

Key Features

@introbul:Key Features @bul:* Comprehensive protocols included for * Detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques * Newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression * Assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone * Calcium imaging by confocal microscopy, evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and -2 message level and distribution

Table of Contents

Enzyme Activity Measurements, Nitric Oxide Detection, and Tissue Localization: Electron Paramagnetic Resonance Detection of Nitric Oxide in Biological Samples, L.G. Rochelle, H. Kruszyna, and R.P. Smith. Electrochemical and Spectroscopic Methods of Nitric Oxide Detection, T. Malinski, E. Kubaszewski, and F. Kiechle. Detection of S-Nitrosothiols, G.N. Welch, G.R. Upchurch, Jr., and J. Loscalzo. Nitrosation of Proteins, Y.-Y. Zhang and J. Loscalzo. Immunohistochemical Methods for Nitric Oxide Synthase, P.K. Nakane NADPH Diaphorase Staining, T.M. Dawson and V.L. Dawson. Immunohistochemical Visualization of Cyclic Nucleotides, J. de Vente, H.M. Young, and H.W.M. Steinbusch. Detection of Downstream Components of Cyclic GMP Cascade by in Situ Hybridization Analysis, M.F. Goy, Z. Li, E.M. Zimmerman, and D.R. Repaske. Detection of Heme Oxygenase-1 and -2 Transcripts by Northern Blot and in Situ Hybridization Analysis, J.F. Ewing. Enzyme Expression, Purification, and Gene Regulation: Purification of Endothelial Nitric Oxide Synthase, J.S. Pollock and M. Nakanev Purification of Brain Nitric Oxide Synthase from Baculovirus Overexpression System and Determination of Cofactors, B. Mayer, P. Klatt, C. Harteneck, B.M. List, E.R. Werner, and K. Schmidt. Prokaryotic Expression, Purification, and Characterization of Intact and Modular Constructs of Neuronal Nitric Oxide Synthase, B.S.S. Masters, K. McMillan, L.J. Roman, P. Martasek, and J. Nishimura. Generation of Monoclonal Antibodies to Endothelial Nitric Oxide Synthase, J.S. Pollock and M. Nakane. Genetic Modulation of Inducible Nitric Oxide Synthase Expression,

Details

No. of pages:
354
Language:
English
Copyright:
© 1996
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080536378
Print ISBN:
9780121853013
Print ISBN:
9780123912138

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