- Methods in Enzymology
- Chapter One: Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites
- 1 INTRODUCTION
- 2 QUANTIFICATION OF 3-NT IN PROTEINS USING HPLC SEPARATION AND ELECTROCHEMICAL (EC) DETECTION
- 3 PROTOCOL FOR QUANTIFICATION OF 3-NT IN HYDROLYZED PROTEINS USING HPLC-EC
- 4 BEYOND QUANTIFICATION: SPECIFICATION OF 3-NT SITES IN PROTEINS
- Chapter Two: Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis
- 1 INTRODUCTION
- 2 DESIGN OF A FLUOROGENIC DERIVATIZATION METHOD
- 3 MODEL STUDIES WITH 4-METHYLCATECHOL AND 2-AMINOCRESOL
- 4 DERIVATIZATION OF A 3-NITROTYROSINE-CONTAINING MODEL PEPTIDE WITH BENZYLAMINE AND 4-AMINOMETHYLBENZENE SULFONIC ACID
- 5 CONCLUSIONS
- Chapter Three: Nitroalkenes: Synthesis, Characterization, and Effects on Macrophage Activation
- 1 INTRODUCTION
- 2 SYNTHESIS AND CHARACTERIZATION OF CHOLESTERYL-NITROLINOLEATE
- 3 SYNTHESIS AND IDENTIFICATION OF NITROALKENES ISOMERS: MASS SPECTROMETRY ANALYSIS OF NITROARACHIDONATE
- 4 DETERMINATION OF LIPID NITRATION DURING MACROPHAGE ACTIVATION
- 5 EFFECTS OF NITROALKENES ON NOS2 INDUCTION BY ACTIVATED MACROPHAGES
- 6 ANALYSIS OF HO-1 INDUCTION AND NF-ΚB ACTIVATION
- Chapter Four: In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods
- 1 INTRODUCTION
- 2 ROLE OF TRACE METAL IONS IN ASCORBATE-MEDIATED REDUCTION OF S-NITROSOTHIOLS
- 3 DETECTION OF PROTEIN S-NITROSATION USING FLUORESCENT LABELING METHODS
- 4 C
The Nobel Prize was awarded in Physiology or Medicine in 1998 to Louis J. Ignarro, Robert F. Furchgott and Ferid Murad for demonstrating the signaling properties of nitric oxide. Nitric oxide (NO) is one of the few gaseous signaling molecules and is a key biological messenger that plays a role in many biological processes. NO research has led to new treatments for treating heart as well as lung diseases, shock and impotence. (Sildenafil, popularly known by the trade name Viagra, enhances signaling through NO pathways.) Scientists are currently testing whether NO can be used to stop the growth of cancerous tumors, since the gas can induce programmed cell death, apoptosis.
This is another “must-have” volume packed with robust methods from authors around the globe. Researchers interested in the detailed biochemistry of NO and its synthesis will have this indispensable volume on their shelves.
*Essential resource for every laboratory involved in NO-related work
*Gathers tried and tested techniques from global labs which eliminates searching through many different sources and avoids pitfalls so the same mistakes are not made over and over.
- Aids researchers in the design of medically important therapies for heart disease and cancer
Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.
- No. of pages:
- © Academic Press 2008
- 26th June 2008
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
University of Southern California, Los Angeles, USA
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.
Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA