Nitric Oxide, Part C: Biological and Antioxidant Activities, Volume 301

1st Edition

Print ISBN: 9780121822026
eBook ISBN: 9780080884127
Imprint: Academic Press
Published Date: 16th October 1998
Page Count: 589


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General Description of the Volume: Nitric Oxide, recently designated "Molecule of the Year," impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume will be a valuable resource for the experienced researcher as well as for those newly entering the field. This volume continues the coverage of new and important tools for the elucidation of Nitric Oxide action initiated in Volumes 268 and 269 of Methods in Enzymology. Techniques for researching the physiology and toxicity of nitric oxide in cellular and organismal systems are highlighted.

General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Biological Activity

  • NO Donors: Nitrosothiols and Nitroxyls
  • Peroxynitrite
  • Oxidant and Antioxidant Action


Researchers in biochemistry, molecular biology, physiology, pharmacology, cell biology, biomedicine, and neuroscience.

Table of Contents

Biological Activity:

J.S. Alexander and N. Okayama, Quantifying Role of Nitric Oxide in Endothelial Barrier Regulation.

M.J. Eppihimer and D.N. Granger, In Vivo Measurements of Endothelial Cell Adhesion Molecule Expression.

P. Kubes, Ruthenium Red as Measure of Mast Cell Activation.

M.K. Shigenaga, Quantitation of Protein-Bound 3-Nitrotyrosine by High-Performance Liquid Chromatography with Electrochemical Detection.

H. Ohshima and H. Bartsch, Quantitative Estimation of Endogenous N-Nitrosation in Humans by Monitoring N-Nitrosoproline in Urine.

D.L. Granger, N.M. Anstey, W.C. Miller, and J.B. Weinberg, Measuring Nitric Oxide Production in Human Clinical Studies.

J.E. Freedman and J.F. Keaney, Jr., Nitric Oxide and Superoxide Detection in Human Platelets.

P. Martásek, R.T. Miller, L.J. Roman, T. Shea, and B.S.S. Masters, Assay of Isoforms of Escherichia coli-Expressed Nitric Oxide Synthase.

E.I. Closs and G.E. Mann, Identification of Carrier Systems in Plasma Membrane of Mammalian Cells Involved in Transport of L-Arginine.

P. Forte, L.M. Smith, E. Milne, and N. Benjamin, Measurement of Nitric Oxide Synthesis in Humans Using L-[15N2]-Arginine.

T.J. McMahon and J.S. Stamler, Concerted Nitric Oxide/Oxygen Delivery by Hemoglobin.

W.K. Alderton and P.K. Lowe, Scintillation Proximity Assay to Measure Nitroarginine and Tetrahydrobiopterin Binding to Heme Domain of Neuronal Nitric Oxide Synthase.

K.-D. Kröncke and V. Kolb-Bachofen, Measurement of Nitric Oxide-Mediated Effects on Zinc Homeostasis and Zinc Finger Transcription Factors.

L.A. MacMillan-Crow and J.A. Thompson, Immunoprecipitation of Nitrotyrosine-Containing Proteins


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© Academic Press 1999
Academic Press
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@from:Praise for the Volume @qu:"This volume is highly recommended for anyone with an interest in studies of oxidants and antioxidants. Since applications in so many different areas are covered, and since the roles of oxidants and antioxidants are so varied and their presence ubiquitous, this volume should be of general usefulness for investigators in many different fields." @source:--Gene A. Homandberg, PhD, Rush Medical College of Rush University for DOODY'S ELECTRONIC JOURNAL @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a def