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1. The expanding toolbox to study the volume regulated anion channel VRAC
2. Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell
3. Optical Methods for Cell Volume Analysis
Michael A. Model
4. Single-molecule imaging of FRET sensors in live budding yeast cells to investigate membrane morphology between mother and daughter cells
5. FRET sensors for membrane tension
6. Methods for studying intracellular liquid-liquid phase transitions
7. Evaluating membrane structure by Laurdan imaging
8. Studying Membrane Organization by Small Angle Neutron Scattering
9. Fluorescent sensors for membrane domains and cholesterol
Francisco J. Barrantes
10. Orthogonal lipid sensors to assess membrane lipid asymmetry
11. Methods for assessment of membrane protrusion dynamics
12. Mass spectrometry-based lipid analysis and imaging
New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest.
- Describes novel FRET-based membrane sensors
- Reviews selected non-optical approaches to membrane structure and dynamics
- Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding
Wide range of researchers in academia and industry
- No. of pages:
- © Academic Press 2021
- 1st November 2021
- Academic Press
- Hardcover ISBN:
Irena Levitan, PhD, is a Professor of Medicine and Bioengineering at the University of Illinois at Chicago. She received her PhD in Biophysics and Neurobiology at the Hebrew University of Jerusalem in 1994 and completed postdoctoral training at the Medical College of Pennsylvania and Institute for Medicine and Engineering at the University of Pennsylvania. Her research focuses on the biophysical properties of endothelial membranes and sub-membrane cytoskeleton. Specifically, the studies of her group, which combine computational and experimental biophysical approaches, provided the first comprehensive structural insights into cholesterol regulation of K+ channels. In 2012, she was named a Guyton Distinguished Lecturer “for her quantitative and biophysical work on cholesterol modulation of ion channels and how this can affect integrated organ function”. She and her group also discovered a paradoxical relationship between fluidity/deformability of the membrane and cell stiffness. In 2018, she was elected a Fellow of AIMBE for “outstanding contributions to our understanding of lipid-ion channel interactions, cellular biomechanics and vascular dysfunction under dyslipidemia”.
Professor of Medicine and Adjunct Professor of Pharmacology and Bioengineering, University of Illinois, Chicago, USA
Michael Model got an M.S. in materials science from Russia and continued his education at the University of Michigan, where he received a Ph.D. in biophysics in 1995. Since 2004, he has been working at Kent State University, first as a microscopy facility manager and currently as an associate professor in the Department of Biological Sciences. While at Kent State, he developed a new microscopic method to measure cell volume, and that prompted him to turn to the physiology of cell volume regulation. He is also interested in the biological role and control of macromolecular crowding and continues to work on microscopic techniques and their applications.
Kent State University
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