Neurons: Methods and Applications for the Cell Biologist

Neurons: Methods and Applications for the Cell Biologist

1st Edition - July 8, 2003
  • Editors: Peter Hollenbeck, James Bamburg
  • eBook ISBN: 9780080496634
  • Hardcover ISBN: 9780123525659

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Description

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.

Key Features

  • Contains culture methodology for more than a dozen types of CNS and PNS neurons
  • Includes most recent and reliable techniques from expert practitioners for specific experimental applications
  • Addresses the latest strategies for transfecting neurons

Readership

Cell and Molecular Biologists, developmental biologists and neuroscientists

Table of Contents

  • Comparing the Properties of Neuronal Culture Systems Growing and Working with Peripheral Neurons
    Dissection and Culturing of Chick Ciliary Ganglion Neurons
    The Culture of Chick Forebrain Neurons
    Growing and Working with Spinal Motor Neurons
    Avian Purkinje Neuronal Cultures
    Culturing Hippocampal and Cortical Neurons
    Working with Xenopus Spinal Neurons in Live Cell Culture
    Culturing Neurons from the Snail Helisoma
    The Ti1 Pioneer Pathway
    Techniques to Dissect Cellular and Subcellular Function in Drosphila Nervous System
    PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells and more.

Product details

  • No. of pages: 500
  • Language: English
  • Copyright: © Academic Press 2003
  • Published: July 8, 2003
  • Imprint: Academic Press
  • eBook ISBN: 9780080496634
  • Hardcover ISBN: 9780123525659

About the Serial Volume Editors

Peter Hollenbeck

Affiliations and Expertise

Purdue University, West Lafayette, U.S.A.

James Bamburg

Affiliations and Expertise

Colorado State University, Fort Collins, U.S.A.