Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas

1st Edition

Molecular Genetics; Lung and Breast Carcinomas

Editors: M. Hayat
Print ISBN: 9780124112803
eBook ISBN: 9780080495194
Imprint: Academic Press
Published Date: 11th May 2004
Page Count: 556
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The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.

This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.

Key Features

  • The only book available that translates molecular genetics into cancer diagnosis
  • Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
  • Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
  • Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
  • Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
  • Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
  • Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References


Graduate and medical students in cancer research, oncology, pathology, biology, immunology, bioinformatics and endocrinology.

Table of Contents

Comparison of Immunohistochemistry, in Situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ Hybridization Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization and Immunohistochemistry Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization Polymerase Chain Reaction DNA Microarrays Technology Tissue Microarrays and their Modifications in Highthoroughput Analysis of Clinical Specimens Gene Expression Profiling Using Laser Microdissection in Cancer Tissues Differential Display of Gene Expression in Human Carcinomas Serial Analysis of Gene Expression in Human Diseases Lung Carcinoma: An Introduction Hitopathological Classification and Phenotype of Lung Tumors Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma Immunohistochemical Expression of MDM2 in Lung Cancer Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma Immunohistochemistry of Laminin-5 in Lung Carcinoma Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma In Situ Hybridization and Immunohistochemistry of Telomerase in Lung Carcinoma Use of Fluorescence in situ Hybridization in Detection of Lung Cancer Cells Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma Breast Carcinoma: An Introduction Expression of Vascular Endothelial Growth Factor Receptor


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Academic Press
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About the Editor

M. Hayat

Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

Affiliations and Expertise

Distinguished Professor, Department of Biological Sciences, Kean University, Union, NJ, USA


"...this handbook is of unparalleled value as a reference book and should be on the shelf of all serious investigators in the field... Its value lies in having an immediate reference source at hand, replete with bibliography, for a particular research project, or occasionally for a particularly difficult clinical issue. In these areas, there are simply no competitors in print..." --Clive R. Taylor, Dept of Pathology, The Keck School of Medicine at the University of Southern California, Los Angeles, in APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY (June 2006) "...this book is a valuable reference for medical students, medical technician, pathologists, and clinician alike who are interested in tumor biology or translational research." --HUMAN PATHOLOGY (July 2005) "Volume 1 of this three-volume Elsevier handbook, edited by M.A. Hayat on the 'Immunohistochemistry and in situ Hybridization of Human Carcinomas' is impressive. ...The technical detail included in many contributions surpasses that available from the scientific and clinical journals; all provide a depth of up-to-date knowledge based upon the authors' experience and expertise. Every large hospital and medical school library should be persuaded to buy a copy of this book." --MICRON (2005) "...Professor Hayat’s handbook, Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas has the great potential of becoming the handbook of choice for clinician and basic scientists including medical and biological students and biomedical technologists." --Akhouri A. Sinha, Ph.D., Professor of Genetics, Cell Biology and Development, University of Minnesota and Research Scientist at the VA Medical Center Minneapolis, MN