Description

This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Table of Contents

General properties and applications of monoclonal antibodies. Assay techniques. Selection of animals and cell lines. Immunisation. Cell culture requirements for hybridomas. Fusion procedures. Transformation. Selection and cloning. Antibody production and purification. Characterisation of monoclonal antibodies.

Details

No. of pages:
264
Language:
English
Copyright:
© 1984
Published:
Imprint:
Elsevier Science
Electronic ISBN:
9780080858821
Print ISBN:
9780444805928

About the author

Reviews

@qu:Clearly written and founded on solid experience and scholarship, this concise book is one of the best sources of practical information on the subject published thus far @source:JOURNAL OF IMMUNOLOGICAL METHODS