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General properties and applications of monoclonal antibodies. Assay techniques. Selection of animals and cell lines. Immunisation. Cell culture requirements for hybridomas. Fusion procedures. Transformation. Selection and cloning. Antibody production and purification. Characterisation of monoclonal antibodies.
This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.
- No. of pages:
- © Elsevier Science 1984
- 1st April 2000
- Elsevier Science
- eBook ISBN:
@qu:Clearly written and founded on solid experience and scholarship, this concise book is one of the best sources of practical information on the subject published thus far @source:JOURNAL OF IMMUNOLOGICAL METHODS