Monoclonal Antibodies - 3rd Edition - ISBN: 9780122870231, 9780080536958

Monoclonal Antibodies

3rd Edition

Principles and Practice

Authors: James Goding
Hardcover ISBN: 9780122870231
eBook ISBN: 9780080536958
Imprint: Academic Press
Published Date: 26th February 1996
Page Count: 492
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Description

Introduction. The Antibody Response. Cellular Basis of the Immune System. Nature of Antigens. Antibody Structure and Function. Genetics of Antibodies. Introduction to Monoclonal Antibodies. Production of Monoclonal Antibodies. Purification. Fragmentation and Isotopic Labeling of Monoclonal Antibodies. Analysis of Antigens Recognized By Monoclonal Antibodies. Affinity Chromatography Immunofluorescence. Immunohistology. Construction, Screening and Expression of Recombinant Antibodies. Generation of Conventional Antibodies.

Key Features

  • Provides an integrated treatment of both the production and application of monoclonals in cell biology, biochemistry, and immunology.
  • Gives detailed and critical accounts of the theory, production, purification, storage, and relabelling of monoclonals, and their use in antigen characterization, affinity chromorography, and immunofluroscence.
  • Comprehensively updated to cover the rapid advances that have occurred since the publication of the Second Edition.

Table of Contents

Introduction. The Antibody Response. Cellular Basis of the Immune System. Nature of Antigens. Antibody Structure and Function. Genetics of Antibodies. Introduction to Monoclonal Antibodies. Production of Monoclonal Antibodies. Purification. Fragmentation and Isotopic Labeling of Monoclonal Antibodies. Analysis of Antigens Recognized By Monoclonal Antibodies. Affinity Chromatography Immunofluorescence. Immunohistology. Construction, Screening and Expression of Recombinant Antibodies. Generation of Conventional Antibodies.

Details

No. of pages:
492
Language:
English
Copyright:
© Academic Press 1996
Published:
Imprint:
Academic Press
eBook ISBN:
9780080536958
Hardcover ISBN:
9780122870231

About the Author

James Goding

Affiliations and Expertise

Monash University, Alfred Hospital, Prahran, Victoria, Australia

Reviews

@from:Reviews of Previous Editions: @qu:"An excellent laboratory manual, invaluable to the newcomer to the field." @source:--NATURE @qu:"Lucid, concise yet comprehensive... the author has managed to execute his task with some brilliancy; it is a pleasure to read or consult the book." @source:--FOLIA BIOLOGICA @qu:"...for those who are involved in the more general production and use of monoclonal antibodies this is an excellent, comprehensive text." @source:--BR F BIOMED SCI