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Molecular Regulation of Nuclear Events in Mitosis and Meiosis presents papers from researchers in various fields engaged in the scientific study of molecular mechanisms involved in the control of nuclear events in meiotic and mitotic cell activity. Various articles in the book discuss a wide range of topics such as the development of cytoplasmic activities that control chromosome cycles during maturation of amphibian oocytes; dynamics of the nuclear lamina during mitosis and meiosis; role of protein phosphorylation in xenopus oocyte meiotic maturation; and cell cycle studies of histone modifications. Molecular and cell biologists, oncologists, and biochemists will find the book invaluable.
1 Development of Cytoplasmic Activities That Control Chromosome Cycles during Maturation of Amphibian Oocytes
II. Oocyte Maturation
III. Maturation-Promoting Factor (MPF)
IV. Cytostatic Factor (CSF)
V. Concluding Remarks
2 Dynamics of the Nuclear Lamina during Mitosis and Meiosis
II. Dynamics of the Nuclear Lamina in Mitosis
III. Dynamics of the Nuclear Lamina during Meiotic Prophase
IV. Dynamics of the Nuclear Lamina during Egg Maturation and Early Development
V. Comparison of the Dynamics of the Nuclear Lamina during Mitosis and Meiosis
3 Regulation of Nuclear Formation and Breakdown in Cell-Free Extracts of Amphibian Eggs
II. Regulation of Pronuclear Formation
III. Regulation of Chromosome Condensation
IV. Role of MPF, Ca2+ Ions, and Protein Phosphorylation in Controlling Nuclear Envelope Breakdown, Chromosome Condensation, and Spindle Assembly in Cell-Free Extracts
4 Role of Protein Phosphorylation in Xenopus Oocyte Meiotic Maturation
II. Maturation-Inhibiting Phosphoproteins (Mp-P) and cAMP
III. Protein Phosphorylation and Maturation-Promoting Factor (MPF) Activity
5 Maintenance of Oocyte Meiotic Arrest by Follicular Fluid Factors
II. Characterization of Follicular Fluid Oocyte Maturation Inhibitor (OMI) by Use of Isolated Mammalian Oocytes
III. Hypoxanthine in Pig Follicular Fluid as the Principal Inhibitor of Mouse Oocyte Maturation and the Prevention of Follicle Stimulating Hormone (FSH) Reversal of cAMP-Maintained Meiotic Arrest
IV. Assay for and Characterization of Human Follicular Fluid OMI by Use of Oocytes from the Amphibian, Xenopus laevis
V. Failure to Inhibit Xenopus Oocyte Maturation by Use of Exogenous Hypoxanthine in Combination with N6,2'-0-Dibutyryl-cAMP (Bt2-Cyclic AMP)
VI. How Do the Inhibitory Factors Present in Follicular Fluid Maintain Meiotic Arrest in Oocytes?
6 Regulation of Chromatin Condensation and Decondensation in Sea Urchin Pronuclei
II. Mitotic and Premature Chromosome Condensation
III. Male Pronuclear Decondensation
7 Regulation of Mitosis by Nonhistone Protein Factors in Mammalian Cells
II. The Mitotic Factors
III. Inhibitors of the Mitotic Factors
IV. Role of Protein Phosphorylation and Dephosphorylation in the Regulation of Nuclear Events Associated with Mitosis and Meiosis
8 Mitosis-Specific Cytoplasmic Protein Kinases
II. Identification of Protein Kinases in Cellular Extracts
III. Cell Cycle Specificity of Kinase Activities
IV. Comparison of Kinase Activities with Protein Kinases Implicated in Mitotic Events
V. Comparison of Kinase Activities with Mitotic Factors
VI. Conclusions and Perspectives
9 Antibodies to Mitosis-Specific Phosphoproteins
II. Monoclonal Antibodies to Cells in Mitosis
III. Characterization of Antigens Reactive with MPM-1 and MPM-2
IV. Cytological Localization of Antigens Reactive with MPM-1 and MPM-2
V. Biological Activity of MPM-1 and MPM-2 Antibodies
10 Mitosis-Specific Protein Phosphorylation Associated with Premature Chromosome Condensation in a ts Cell Cycle Mutant
II. Isolation and Preliminary Characterization of the tsBN2 Cell Line
III. Induction of Premature Chromosome Condensation (PCC) in tsBN2 Cells at the Nonpermissive
IV. Induction of Mitosis-Specific Phosphorylation in tsBN2 Cells by Temperature Shift
V. Newly Synthesized Protein(s) in tsBN2 Cells Showing PCC
11 Chromatin Structure and Histone Modifications through Mitosis in Plasmodia of Physarum polycephalum
II. Chromatin Structure and Organization
III. Control of Chromosome Condensation
IV. Cell Cycle Studies of Histone Modifications
V. Histone Hl°
VI. Histone HI Kinases
- No. of pages:
- © Academic Press 1987
- 17th February 1987
- Academic Press
- eBook ISBN:
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