General Description of the Volume: This Methods in Enzymology volume continues the description (initiated in Volume 85) of the purification and assay of this diverse group of proteins involved in cytoplasmic organization. It discusses some of the more promising attempts to apply techniques of molecular biology genetics and molecular cytochemistry to these proteins.
General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
@introbul:Key Features @bul:* Major topics covered are:
- Analysis of Actinomyosin-Related Systems and of Microtubule-Related Systems
- Other Cytoskeletal Systems
- Cell-Free and Genetic Systems
- Force Production Assays
- General Methods
Analysis of Actomyosin-Related Systems:
R.E. Cheney, Purification and Assay of Myosin V.
K. Ayscough, Use of Latrunculin-A, An Actin Monomer Binding Drug.
M.R. Bubb and I. Spector, Use of the F-Actin Binding Drugs, Misakinolide A and Swinholide A.
E.J. Luna, F-Actin Blot Overlays.
J.F. Kelleker, R.D. Mullins, and T.D. Pollard, Purification and Assay of the Arp2/3 Complex from Acanthamoeba castellani.
M.D. Welch and T.J. Mitchison, Purification and Assay of the Platelet Arp2/3 Complex.
K.L. Schmeichel, B.E. Stronach, and M.C. Beckerle, Purification and Assay of Zyxin.
C.E. Turner and M.C. Brown, Purification and Assays for Paxillin.
J. Lacoste, A. Ma, and J.T. Parsons, Assay and Purification of Focal Adhesion Kinase.
T. Jarchau, T. Mund, M. Reinhard, and U. Walter, Purification and Assays of VASP.
J.A. Theriot and D.C. Fung, Listeria monocytogenes-based Assays for Actin Assembly Factors.
Analysis of Microtubule-Related Systems:
A. Desai and T.J. Mitchison, Preparation and Characterization of Caged Fluorescein Tubulin.
D. Meyer, D.R. Rines, A. Kashina, and J.M. Scholey, Purification of Novel Kinesins from Embryonic Systems.
D.W. Pierce and R.D. Vale, Assaying Processive Movement of Kinesin by Fluorescence Microscopy.
J.B. Bingham, S.J. King, and T.A. Schroer, Purification of Dynactin and Dynein from Brain Tissue.
M.P. Sheetz and J. Kumar, Isolation and Characterization of Kinectin.
G.S. Diamantopoulos, T.E. Kreis, and J.E. Rickard, Purification and Assay of CLIP-170.
F. McNally, Purification and Assay of the Microtu
- No. of pages:
- © Academic Press 1998
- 24th August 1998
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
- Paperback ISBN:
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts, U.S.A.
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY