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Molecular Microbiology Laboratory, Second Edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level underg… Read more
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Introduction
Experiment 1. Plasmid Purification and Restriction Mapping
I Introduction
II Background: Plasmid DNA Preparation
III Procedure
IV Laboratory Report [Class 4 (Draft) and Class 5]
V Questions (Class 5)
VI In-Class Writing Exercise (Class 2)
VII Restriction Mapping Exercises (Class 3)
VIII In-Class Writing Exercise (Class 4)
Experiment 2. Affinity Purification of Histidine-Tagged FnbA Protein
I Introduction
II Background
III Procedure
IV Laboratory Report
V Critique of Paper by Bush et al.
Experiment 3. Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes
I Introduction
II Background
III Procedure
IV Laboratory Report [Class 16 (Draft) and Class 17]
V Questions (Class 17)
VI In-Class Writing Exercise (Class 10)
Experiment 4. Terminal Restriction Fragment Length Polymorphism Analysis of 16S Ribosomal RNA Genes from Uncultured Bacterial Communities
I Introduction
II Background
III Procedure
IV Laboratory Report
V Questions (Class 20)
VI Writing Exercise: Editorial on Genetically Modified Crops [Class 14 (Draft) and Class 15]
VII Writing Assignment: Literature Search Problem [Class 14 (Begin) and Class 20]
VIII In-Class Writing Exercise (Class 15)
APPENDIX A: Sample Laboratory Reports
APPENDIX B: Sample Proposals
APPENDIX C: Sample Tests
APPENDIX D: Guidance for Presentations
APPENDIX E: Revised Paragraph from J. Biol. Chem. 266: 3811–3814 (1991)
APPENDIX F: Abstract from Appl. Environ. Microbiol. 63: 2647–2653 (1997)
APPENDIX G: Sequence Editing Tutorial
APPENDIX H: Peak Scanner Tutorial
APPENDIX I: Adding Size Standards to Peak Scanner
APPENDIX J: PRIMER Tutorial
APPENDIX K: Checklist for Grading Laboratory Reports
APPENDIX L: Peer Review Checklist
APPENDIX M: Career Advice
APPENDIX N: Molecular Microbiology Laboratory Preparation Manual
Bibliography
Index
BG
KF