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Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.
- Exposes students to the new molecular-based techniques
- Provides faculty with an authoritative, accessible resource for teaching protocols
- The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum
- Based on a successful course for which the author won a teaching award
- Presents a real-world study of bacterial populations in the environment in the final experiment
- Provides an overview of molecular biology in a new review chapter
- Demonstrates how to design an experiment and how to interpret the results
- Covers grant proposal writing and how panels review proposals
- Presents guidance on public speaking and preparing PowerPoint presentations
- Includes tutorials on three widely used software packages
Appropriate for college sophomores through seniors in any of the life sciences, including microbiology, genetics, general biology, biochemistry, molecular biology, botany, zoology, etc. The experiments are also appropriate for properly equipped junior colleges and liberal arts institutions.
Experiment 1. Plasmid Purification and Restriction Mapping
II Background: Plasmid DNA Preparation
IV Laboratory Report [Class 4 (Draft) and Class 5]
V Questions (Class 5)
VI In-Class Writing Exercise (Class 2)
VII Restriction Mapping Exercises (Class 3)
VIII In-Class Writing Exercise (Class 4)
Experiment 2. Affinity Purification of Histidine-Tagged FnbA Protein
IV Laboratory Report
V Critique of Paper by Bush et al.
Experiment 3. Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes
IV Laboratory Report [Class 16 (Draft) and Class 17]
V Questions (Class 17)
VI In-Class Writing Exercise (Class 10)
Experiment 4. Terminal Restriction Fragment Length Polymorphism Analysis of 16S Ribosomal RNA Genes from Uncultured Bacterial Communities
IV Laboratory Report
V Questions (Class 20)
VI Writing Exercise: Editorial on Genetically Modified Crops [Class 14 (Draft) and Class 15]
VII Writing Assignment: Literature Search Problem [Class 14 (Begin) and Class 20]
VIII In-Class Writing Exercise (Class 15)
APPENDIX A: Sample Laboratory Reports
APPENDIX B: Sample Proposals
APPENDIX C: Sample Tests
APPENDIX D: Guidance for Presentations
APPENDIX E: Revised Paragraph from J. Biol. Chem. 266: 3811–3814 (1991)
APPENDIX F: Abstract from Appl. Environ. Microbiol. 63: 2647–2653 (1997)
APPENDIX G: Sequence Editing Tutorial
APPENDIX H: Peak Scanner Tutorial
APPENDIX I: Adding Size Standards to Peak Scanner
APPENDIX J: PRIMER Tutorial
APPENDIX K: Checklist for Grading Laboratory Reports
APPENDIX L: Peer Review Checklist
APPENDIX M: Career Advice
APPENDIX N: Molecular Microbiology Laboratory Preparation Manual
- No. of pages:
- © Academic Press 2012
- 31st August 2012
- Academic Press
- Paperback ISBN:
- eBook ISBN:
Walt Ream, Professor of Microbiology, Oregon State University, received the Richard M. Bressler Senior Faculty Teaching Award for developing the course this book supports. In addition to this work, he has written numerous peer reviewed scholarly papers, review articles, book articles and chapters and has co-authored another book entitled, Molecular Biology Techniques: An Intensive Laboratory Course. His research led to a discovery for which a patent was issued in 2004. He regularly reviews grant proposals for NSF, USDA, DOE, NIH, American Cancer Society, NSERC-Canada, Lindbergh Foundation, Corporation for Plant Biotechnology Research and he reviews papers for Journal of Bacteriology, Plant Molecular Biology, PNAS, Science, Molecular & General Genetics, Microbiological Reviews, Molecular Plant-Microbe Interactions, Biochemistry, Journal of Biological Chemistry, Plasmid, Gene, Plant Physiology, Journal of American Horticulture Society, and Molecular Microbiology. He is a member of Genetics Society of America, American Society for Microbiology, and American Association for the Advancement of Science.
Professor, Department of Microbiology, Oregon State University
Associate Professor, Department of Microbiology, Oregon State University
"This book is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports."--Anticancer Research, August 2013
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