Molecular Cloning and Gene Regulation in Bacilli - 1st Edition - ISBN: 9780122741500, 9780323153096

Molecular Cloning and Gene Regulation in Bacilli

1st Edition

Editors: A Ganesan
eBook ISBN: 9780323153096
Imprint: Academic Press
Published Date: 28th January 1982
Page Count: 382
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Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation. Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid vector of large DNA segments, and the mating system of bacilli. Molecular cloning session covers complementation system and dominance analyses in Bacillus, genetic fusion of Escherichia coli lac genes to a Bacillus subtilis promoter, and DNA cloning of B. subtilis. It also describes the construction of trimethoprim resistant B. subtilis plasmid and expression of E. coli trp genes cloned in B. subtilis. Session III encompasses chapters that discuss protein secretion by bacilli; regulation of alpha-amylase production in B. subtilis; entomocidal toxin translation of B. thuringiensis; and expression of crystal protein, heterologous, and eukaryotic genes in bacilli. Session IV focuses on various aspects of DNA metabolism of bacilli, such as the interaction of bacterial chromosome with cell membrane; plasmid DNA in competent cells and protoplasts of B. subtilis; analysis of peptides synthesized by B. subtilis mutants; and DNA repair, uptake, restriction, modification, and recombination. The final session examines species-specific translation, control of gene expression and replications in plasmids, development of expression-vector in B. subtilis, and regulatory modifications of RNA polymerase. Each chapter is presented in an experimental manner, consisting of a summary of the study, materials and methods, results, as well as references.

Table of Contents

Contributors Preface Opening Remarks Session I Chromosome Structure and Gene Arrangement Nucleotide Sequence of Intergenic Spacer DNA of Three 16S-23S RNA Gene Sets from B. subtilis Use of a Bifunctional Cosmid for Cloning Large DNA Fragments of B. subtilis B. subtilis Mating System: Dicaryotic Recombinants and Suppression of the Prototrophic Phenotype Genetic Manipulations with Phage SPB Session II Molecular Cloning System for Complementation and Dominance Analyses in Bacillus Genetic Fusion of E. coli lac Genes to a B. subtilis Promoter DNA Cloning in B. subtilis. IV. Transformation of Competent Cells by Internally Repeated Plasmids Construction of a Trimethoprim Resistant B. subtilis Plasmid for Use in Cloning the E. coli trpC Gene in B. subtilis Expression of E. coli trp Genes Cloned in B. subtilis Session III Expression of Cloned Genes Penicillinase and the Secretion of Proteins by Bacilli Regulation of α-Amylase Production in B. subtilis In Vitro Translation of the Entomocidal Toxin of B. thuringiensis Cloning the Crystal Protein Gene of B. thuringiensis in E. coli Expression and Instability of Heterologous Genes in B. subtilis Expression of Eukaryotic Genes in B. subtilis Using Signals of penP Session IV DNA Metabolism Initiation of Chromosome Replication and Membrane Association of the Replication Origin in B. subtilis Characterization of the Chromosomal Terminus of B. subtilis and its Attachment to the Cell Membrane Characterization of the Terminus Region of the B. subtilis Chromosome Fate of Plasmid DNA in Competent Cells and Protoplasts of B. subtilis Coordinated Changes in Competence Associated Polypeptides among Transformation Defective Mutants of B. subtilis DNA Repair in B. subtilis: The Identification of a Strain Temperature Inducible for 'SOS' Functions DNA-Dependent ATPases in B. subtilis: Properties and Functions Upta


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© Academic Press 1982
Academic Press
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About the Editor

A Ganesan

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