Molecular Biology of RNA

Molecular Biology of RNA

New Perspectives

1st Edition - December 28, 1987

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  • Editors: Masayori Inouye, Bernard S. Dudock
  • eBook ISBN: 9781483273907

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Description

Molecular Biology of RNA: New Perspectives provides an overview of the developments in RNA research as well as the approaches, strategies, and methodologies used. Most of the contributing authors in the present volume participated in the Fifth Stony Brook Symposium entitled "New Perspectives on the Molecular Biology of RNA" in May 1986. The text is organized into six parts. Part I contains papers dealing with RNA as an enzyme. Part II presents studies on RNA splicing. Part III examines RNA viruses while Part IV focuses on the role of RNA in DNA replication. Part V is devoted to the structure, function, and isolation of RNA. Finally, Part VI takes up the role of RNA in regulation and repression. This volume will help provide new direction and insight for those already working on the subject and will serve as a useful guide to those about to start research in the molecular biology of RNA.

Table of Contents


  • Preface

    I RNA as an Enzyme

    1 Cleavage of RNA by RNAse P from Escherichia coli

    I. Introduction

    II. Aspects of the RNase P Reaction

    III. Studies of Enzyme-Substrate Interactions

    IV. Structure-Function Relationships in Ml RNA

    V. Studies of the Protein Subunit of RNase P

    VI. Hybrid Enzymes

    VII. Conclusion

    References

    2 Bacillus Subtilis RNase

    I. The RNase P Components

    II. Mechanism of RNase P Cleavage

    III. Toward the Higher-Order Structure of RNase P RNA

    IV. Structure-Function Relationships in the RNase P RNA

    V. Why Is the Catalytic Element of RNase P Composed of RNA?

    References

    3 Multiple Enzymatic Activities of an Intervening Sequence RNA from Tetrahymena

    I. Introduction

    II. Self-Splicing RNA

    III. The IVS RNA Enzyme

    References

    4 Processing and Genetic Characterization of Self-Splicing RNAs of Bacteriophage

    I. Introduction

    II. Group I Splicing Mechanism for T4 td RN

    III. Nondirected Mutagenesis and Delineation of Two Functional Domains for Splicing in the td Intron

    IV. Multiple Self-Splicing Introns in T4

    V. Conclusions

    References

    II RNA Splicing

    5 The Mammalian Pre-Messenger RNA Splicing Apparatus: A Ribosome in Pieces?

    I. Introduction

    II. The Discovery of snRNPs

    III. The snRNPs-and-Splicing Hypothesis

    IV. snRNP Components and Structure

    V. Is Eukaryotic RNase P an Sm snRNP?

    VI. U1 snRNPs Bind 5' Splice Sites

    VII. U2, U5, and U4/U6 snRNPs Also Participate in Splicing

    VIII. The Spliceosome-Ribosome Analogy

    References

    6 Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection

    I. Introduction

    II. Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection

    III. The Pattern of Splice Site Selection Is Altered in Different Extract Preparations and in Diluted Extracts

    IV. Splice Site Selection Can Be Altered by Competition in Trans

    V. Discussion

    References

    7 Factors That Influence Alternative Splice Site Selection in Vitro

    I. Introduction

    II. Materials and Methods

    III. Results

    IV. Discussion

    References

    8 Messenger RNA Splicing in Yeast

    I. An Overview of Nuclear mRNA Splicing

    II. Preliminary in Vitro and in Vivo Characterization of Yeast mRNA Splicing

    III. Characterization of Mutations in the Splicing Process

    IV. The RNA Gene Products and the Spliceosome

    V. Speculations

    References

    9 Architecture of Fungal Introns: Implications for Spliceosome Assembly

    I. Introduction

    II. Branch Site-3' Splice Junction Relationship

    III. Branch Site-5' Splice Junction Relationship

    IV. Perspectives

    References

    10 RNA Joining and Trypanosome Gene Expression

    I. Introduction

    II. Materials and Methods

    III. Results

    IV. Discussion

    V. Summary

    References

    III RNA Viruses

    11 The Polio virus Genome: A Unique RNA in Structure, Gene Organization, and Replication

    I. Introduction

    II. Translation and Processing of the Polyprotein

    III. RNA Replication

    IV. Conclusions

    References

    12 Permanent Expression of Influenza Virus Genes Coding for Transcriptase Complexes: Complementation of Viral Mutants

    I. Establishment of a Functional Expression System

    II. Addition of Nuclear Protein to the Transcription Complex

    III. Conclusions and Outlook

    References

    13 Molecular Mechanisms of Pathogenesis

    Text

    References

    IV RNA in DNA Replication

    14 Changes in RNA Secondary Structure May Mediate the Regulation of IncFII Plasmid Gene Expression and DNA Replication

    I. Introduction

    II. RNA Secondary Structure Predictions

    III. Discussion

    References

    15 Regulation of Co IE 1 DNA Replication by Antisense RNA

    I. Introduction

    II. RNA Primer Formation

    III. Regulation of Primer Formation

    IV. Binding of RNA I to RNA II

    V. Importance of the Rate of Binding of RNA I to RNA II

    VI. Secondary Structure of RNA II and Its Alteration by Binding of RNA I

    VII. Conclusions

    References

    16 A Transfer RNA Implicated in DNA Replication

    I. Introduction

    II. Expression of the DNA Y Gene

    III. Implication in DNA Replication

    IV. Summary and Models for Replication Role

    References

    V RNA: Structure, Function, and Isolation

    17 Stable Branched RNA Covalently Linked to the 5' End of a Single-Stranded DNA of Myxobacteria

    I. Introduction

    II. DNA Structure of Stigmatella aurantiaca msDNA

    III. RNA Sequence of RNA-Linked msDNA

    IV. Determination of the Linkage between RNA and msDNA

    V. Gene Arrangement of the Coding Regions for msDNA (msd) and msdRNA (msr) on the Chromosome

    VI. Biosynthesis of Branched RNA-Linked msDNA

    VII. How Is Branched RNA-Linked msDNA Synthesized?

    References

    18 Recognition of RNA by Proteins

    I. A Simple RNA-Protein Interaction

    II. A Complex RNA-Protein Interaction

    References

    19 A New Role for Transfer RNA: A Chloroplast Transfer RNA Is a Cofactor in the Conversion of Glutamate to Delta-Aminolevulinic Acid

    I. Introduction

    II. RNA DALA Is a Unique Glutamine-Accepting tRNA

    III. RNA as a Cofactor: Possible Reaction Mechanism

    IV. Outlook

    References

    20 Natural Suppressor Transfer RNA in Eukaryotes: Its Implication in the Evolution of the Genetic Code and Expression of Specific Genes

    I. Introduction

    II. Isolation from Tetrahymena of the tRNA Gene and tRNA Corresponding to the Termination Codon UAA

    III. Deviation of the Genetic Code of Tetrahymena from the Universal Genetic Code

    IV. Evolution of Glutamine tRNAs Recognizing UAA and UAG Termination Codons in Tetrahymena

    V. Isolation of a Natural UAG Suppressor Glutamine tRNA from Mouse Cells

    VI. A Large Increase of tRNAg[;UG in Mouse Cells Infected with Mo-MuLV

    VII. Concluding Remarks

    References

    21 The Purification of Small RNAs by High-Performance Liquid Chromatography

    I. Introduction

    II. Materials and Methods

    III. Results

    IV. Discussion

    References

    22 Comparative Studies on the Secondary Structure of the RNAs of Related RNA Coliphages

    I. Introduction

    II. Results

    III. Discussion

    References

    VI RNA in Regulation and Repression

    23 Autogenous Regulation of Transcription of the crp Operon by a Divergent RNA Transcript

    I. Introduction

    II. Activation of a Divergent Promoter by Cyclic AMP-CRP is Required for crp Autoregulation

    III. Autoregulation of crp Is Mediated by Divergent RNA

    IV. A Model for crp Autoregulation

    V. Conclusion

    References

    24 The Role of Translational Regulation in Growth Rate-Dependent and Stringent Control of the Synthesis of Ribosomal Proteins in Escherichia Coli

    I. Secondary Structure of the LI Target Site on L11 mRNA

    II. Growth Rate-Dependent Control of Ribosomal Protein Synthesis

    III. Stringent Control of Ribosomal Protein Synthesis

    IV. Concluding Remarks

    References

    25 Sequence and Structural Elements Associated with the Degradation of Apolipoprotein II Messenger RNA

    I. Introduction

    II. Effect of 5' Noncoding Sequences on apo II mRNA Stability

    III. Use of Enzymatic Probes and Reverse Transcriptase to Analyze apo II mRNA Secondary Structure

    IV. Analysis of apo II mRNA in Polysomes

    V. Summary and Perspective

    References

    26 A New Immune System against Viral Infection Using Antisense RNA: micRNA-Immune System

    I. Introduction

    II. micRNA Mutagenesis

    III. micRNA-Immune System

    IV. Conclusion

    References

    27 Regulation of IS10 Transposase Expression by RNA/RNA Pairing

    I. Introduction

    II. Biological Role of Multicopy Inhibition

    III. Molecular Mechanism of Multicopy Inhibition

    IV. Mechanism of Pairing between RNA-IN and RNA-OUT In Vitro

    V. In Vivo Phenotypes of Mutations in RNA-OUT

    References

    28 Characterization and Functional Analysis of the Factors Required for Transcription of the Adenovirus Major Late Promoter

    I. Introduction

    II. Fractionation and Functional Analysis of the Factors Required for Transcription of the Adenovirus Major Late Promoter

    III. Concluding Remarks

    References

    Index


Product details

  • No. of pages: 464
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: December 28, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9781483273907

About the Editors

Masayori Inouye

Affiliations and Expertise

Robert Johnson Medical School, Picataway, New Jersey, U.S.A.

Bernard S. Dudock

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