Molecular and Diagnostic Procedures in Mycoplasmology

Molecular and Diagnostic Procedures in Mycoplasmology

Diagnostic Procedures

2nd Edition - February 13, 1996

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  • Editors: Joseph Tully, Shmuel Razin
  • eBook ISBN: 9780080536798

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This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.

Key Features

  • Diagnostic genetic probes
  • Immunological tools
  • Antibiotic sensitivity testing
  • Diagnosis of specific diseases
  • Experimental infections
  • Diagnosis of mycoplasma infections of cell cultures


Microbiologists, virologists, molecular biologists, clinicians, and plant pathologists.

Table of Contents

  • J.G. Tully, Mollicute-Host Interrelationships: Current Concepts and Diagnostic Implications.
    Diagnostic Genetic Probes:
    S. Razin, Introductory Remarks.
    K.-E. Johansson, Oligonucleotide Probes Complementary to 16S rRNA.
    D. Yogev and S. Razin, Cloned Genomics DNA Fragments as Probes.
    R. Kovacic, O. Grau, and A. Blanchard, PCR: Selection of Target Sequences.
    B. de Barbeyrac, C. Bebear, and D. Taylor-Robinson, PCR: Preparation of DNA from Clinical Specimens.
    B. de Barbeyrac and C. Bebear, PCR: Amplified and Identification of Products.
    R. Harasawa, PCR: Application of Nested PCR to Detection of Mycoplasmas.
    S.J. Geary and M. Forsyth, PCR: Random Amplified Polymorphic DNA Fingerprinting.
    Immunological Tools:
    J.G. Tully, Introductory Remarks.
    M.B. Brown, J.M. Bradbury, and J.K. Davis, ELISA in Small Animals, Rodents, and Birds.
    J. Nicolet and J.L. Martel, ELISA in Large Animals.
    R.Y.-H. Wang and S.-C. Lo, ELISA in Human Urogenital Infections and AIDS.
    G.H. Cassell, G. Gambil, and L. Duffy, ELISA in Respiratory Infections of Humans.
    C.B. Thomas, M. Garnier, and J.T. Boothby, Monoclonal Antibodies as Diagnostic Tools.
    D. Taylor-Robinson, Microimmunofluorescence.
    D. Thirkell and B.L. Precious, Immunoblots and Immunobinding.
    J.G. Tully, Differentiation of Mycloplasma genitalium from Mycoplasma pneumoniae by Immunofluoresence.
    Antibiotic Sensitivity Testing:
    C. Bebear, Introductory Remarks.
    G.E. Kenny, Problems and Opportunities in Susceptibility Testing of Mollicutes.
    C. Bebear and J.A. Robertson, Determination of Minimal Inhibitory Concentration.
    D. Taylor-Robinson, Cidal Activity Testing.
    Diagnosis of Specific Diseases:
    J.G. Tully, Introductory Remarks.
    R.J. Harris, J. Williamson, C. Hahn, and B.P. Marmion, Laboratory Diagnosis of Mycoplasma pneumoniae Infections.
    D. Taylor-Robinson, Diagnosisof Sexually Transmitted Diseases.
    K.B. Waites and G.H. Cassell, Diagnosis of Neonatal Infections.
    S.-G. Lo, Mycoplasmas in AIDS Patients.
    E.A. ter Laak and H.L. Ruhnke, Mycoplasma Infections of Cattle.
    A.J. DaMassa, Mycoplasma Infections of Goats and Sheep.
    R.F. Ross and G.W. Stemke, Mycoplasma Infections of Swine.
    S.H. Kleven and S. Levisohn, Mycloplasma Infections of Poultry.
    C. Saillard, C. Barthe, J.M. Bove, and R.F. Whitcomb, Diagnosis of Spiroplasma Infections in Plants and Insects.
    E. Seemueller and B.C. Kirkpatrick, Detection of Phytoplasma Infections in Plants.
    R.F. Whitcomb and K.J. Hackett, Identification of Mollicutes from Insects.
    Experimental Infections:
    J.G. Tully, Introductory Remarks.
    G.H. Cassell and A. Yancey, Experimental Mycoplasmal Respiratory Infections in Rodents.
    P.M. Furr and D. Taylor-Robinson, Urogenital Infections in Rodents.
    L.R. Washburn, Experimental Models of Arthritis.
    J.M. Bradbury and S. Levisohn, Experimental Infections in Poultry.
    M. Kobisch and R.F. Ross, Experimental Infections of Swine.
    R.F. Rosenbusch and H.L. Ruhnke, Experimental Infections in Cattle.
    X. Foissac, J.L. Danet, C. Saillard, R.F. Whitcomb, and J.M. Bove, Experimental Infections of Plants by Spiroplasmas.
    A.H. Purcell, Experimental Phytoplasma Infections in Plants and Insects.
    S.-C. Lo and A. Blanchard, Mycoplasmas and in Vitro Infections of Cell Cultures with HIV.
    Diagnosis of Mycoplasma Infections of Cell Cultures:
    J.G. Tully, Introductory Remarks.
    R.A. Del Giudice and J.G. Tully, Isolation of Mycoplasmas from Cell Culture by Axenic Cultivation Techniques.
    G.K. Masover and F.A. Becker, Detection of Mycoplasmas by DNA Staining and Fluorescent Antibody Methodology.
    C. Veilleux, S. Razin, and L.H. May, Detection of Mycoplasma Infection by PCR.
    R.A. Del Giudice and R.S. Gardella, Antibiotic Treatment of Mycoplasma-Infected Cell Cultures.
    A.Smith and J. Mowles, Prevention and Control of Mycoplasma Infections of Cell Cultures.
    Subject Index.

Product details

  • No. of pages: 466
  • Language: English
  • Copyright: © Academic Press 1996
  • Published: February 13, 1996
  • Imprint: Academic Press
  • eBook ISBN: 9780080536798

About the Series Editors

Joseph Tully

Affiliations and Expertise

Frederick Cancer Research Center, Frederick, Maryland, U.S.A.

Shmuel Razin

Affiliations and Expertise

The Hebrew University-Hadassah Medical School, Jerusalem, Israel

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