Molecular and Diagnostic Procedures in Mycoplasmology

1st Edition

Molecular Characterization

Series Volume Editors: Shmuel Razin Joseph Tully
Hardcover ISBN: 9780125838054
eBook ISBN: 9780080536781
Imprint: Academic Press
Published Date: 8th December 1995
Page Count: 483
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This book and its companion, Volume II, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. Volume II deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures, with particular emphasis on the association of mycoplasmas with the activation of AIDS.

Key Features

@introbul:Key Features @bul:* Cultivation and morphology

  • Genome characterization and genetics
  • Membrane characterization
  • Cell metabolism
  • Taxonomy and phylogeny
  • Pathogenicity


Microbiologists, virologists, molecular biologists, cell biologists, clinicians, and plant pathologists.

Table of Contents

S. Razin, Molecular Properties of Mollicutes: A Synopsis.

Cultivation and Morphology:

J.G. Tully, Introductory Remarks.

J.G. Tully, Culture Medium Formulation for Primary Isolation and Maintenance of Mollicutes.

K.J. Hackett and R.F. Whitcomb, Cultivation of Spiroplasmas in Undefined and Defined Media.

K.J. Hackett and D.E. Lynn, Insect Cell Culture Approaches in Cultivating Spiroplasmas.

J.A. Robertson and G.W. Stemke, Measurement of Mollicutes Growth by ATP-Dependent Luminometry.

D.Taylor-Robinson, Intracellular Location of Mycoplasmas.

D.J. Wear and S.-C. Lo, Localization of Mycloplasmas in Tissues.

D.C. Krause and M.K. Stevens, Localization of Antigens on Mycoplasma Cell Surface and Tip Structures.

Genome Characterization and Genetics:

S. Razin, Introductory Remarks.

B.C. Kirkpatrick, N.A. Harrison, I.-M. Lee, H. Neimark, and B.B. Sears, Isolation of Mycoplasma-Like Organism DNA from Plant and InsectHosts.

H. Neimark and P. Carle, Mollicute Chromosome Size Determination and Characterization of Chromosomes from Uncultured Mollicutes.

T. Proft and R. Herrmann, Physical and Genetic Mapping.

K. Dybvig, Characterization of Virus Genomesand Extrachromosomal Elements.

J. Renaudin and J.-M. Bove, Plasmid and Viral Vectors for Gene Cloning and Expression in Sprioplasma citri.

K. Dybvig, G.E. Gasparich, and K.W. King, Artificial Transformation of Mollicutes via Polyethylene Glycol- and Electroporation-Mediated Methods.

A. Razin and P. Renbaum, DNA Methylation Analysis.

B. Bhugra and K. Dybvig, Identification and Characterization of Genom


No. of pages:
© Academic Press 1995
Academic Press
eBook ISBN:
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About the Series Volume Editor

Shmuel Razin

Affiliations and Expertise

The Hebrew University-Hadassah Medical School, Jerusalem, Israel

Joseph Tully

Affiliations and Expertise

Frederick Cancer Research Center, Frederick, Maryland, U.S.A.