Molecular and Cell Biology

Molecular and Cell Biology

1st Edition - January 1, 1978

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  • Editor: Harry Gelboin
  • eBook ISBN: 9780323149068

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Description

Polycyclic Hydrocarbons and Cancer, Volume 2: Molecular and Cell Biology explores the link between polycyclic aromatic hydrocarbons (PAHs) and cancer from the viewpoint of molecular and cell biology. This volume consists of 26 chapters divided into eight sections based on the following themes: DNA and Chromatin Interactions; Microbial Mutagenesis; Mammalian Mutagenesis; DNA Repair; Transformation of Cells in Culture; Animal and Human Models; Genetics; and Comments and Observations. The discussion begins with an analysis of the interactions of PAHs with cellular macromolecules and with DNA of mammalian cells in culture. The following chapters focus on microbial and mammalian mutagenesis by PAHs; mechanisms of DNA excision repair in human cells; effect of DNA repair on the cytotoxicity and mutagenicity of PAH metabolites in human cells; and oncogenic transformation of cell cultures by PAHs and their derivatives. The final chapter makes the case for the establishment of a system for conducting quantitative assessments of biomedical risk presented by many types of environmental perturbants such as PAHs. This monograph will be of interest to investigators and educators concerned with scientific aspects of PAH research; government officials and elected representatives as well as industry leaders who must confront and solve the problems related to PAHs; and others in the field of molecular and cell biology.

Table of Contents


  • List of Contributors

    Preface

    Contents of Volume 1

    Part I DNA and Chromatin Interactions

    1 Interactions between Polycyclic Aromatic Hydrocarbons and Cellular Macromolecules

    I. Introduction

    II. Structures of PAH-Nucleoside Adducts

    III. Conformation of DNA Modified by Reaction with BPDE

    IV. Functional Changes in BPDE-Modified DNA

    V. Reactions of PAH's with Protein

    VI. Summary and Unresolved Problems

    References

    2 Recent Work of Pascaline Daudel

    I. Introduction

    II.Interaction of Carcinogens with DNA: Resulting Damages

    References

    3 The Interaction of Polycyclic Hydrocarbons with DNA of Mammalian Cells in Culture

    I. Introduction

    II. Methodology

    III. The In Vivo Binding of BP, MBA, and MC to DNA

    References

    4 Reactions of Activated Benzo[a]Pyrene with DNA and RNA

    Text

    References

    5 Modification of DNA by Benzo[a]Pyrene Diol Epoxide I

    I. Introduction

    II. Materials and Methods

    III. Results and Discussion

    References

    6 Interactions between Benzo[a]Pyrene Diol Epoxide I and Chromatin

    I. Introduction

    II. Modification of Core Particles with BPDE

    III. Modification of Core Particle DNA with BPDE

    IV. Modification of Core Particle Histones with BPDE

    V. Modification of Nonhistone Core Particle Proteins with BPDE

    VI. Conclusion

    References

    Part II Microbial Mutagenesis

    7 Mutagenesis: Microbial Systems

    I. Introduction

    II. Microbial Assay System

    III. Mutagenicities of Benzo[a]Pyrene and Its Metabolites

    IV. Polycyclic Aromatic Hydrocarbons Other than Benzo[a]Pyrene

    V. Polycyclic Aromatic Hydrocarbons as Environmental Mutagens

    References

    8 Comparative Mutagenicity of Diol Epoxides of Benzo[a]Pyrene and Benzo[a]Anthracene in V79 Chinese Hamster Cells and Salmonella Typhimurium

    I. Introduction

    II. Experimental Procedures

    III. Results and Discussion

    IV. Concluding Remarks

    References

    Part III Mammalian Mutagenesis

    9 Mammalian Cell Mutagenesis by Polycyclic Aromatic Hydrocarbons and Their Derivatives

    I. Introduction

    II. Mutagenic Activity of Carcinogenic PAH's

    III. Conclusion

    References

    10 Cell Transformation and Mutability of Different Genetic Loci in Mammalian Cells by Metabolically Activated Carcinogenic Polycyclic Hydrocarbons

    I. Introduction

    II. Methods

    III. Results

    IV. Conclusion

    References

    Part IV DNA Repair

    11 Mechanisms of DNA Excision Repair in Human Cells

    I. Introduction

    II. Repair Reactions in Cells

    III. Mechanism of Excision Repair

    IV. Repair of Benzopyrene Diol Epoxide-Induced Damage

    V. Adherence to BND-Cellulose by Chemical Interaction

    VI. Excision Repair and Genetic Processes

    VII. Control of DNA Repair Capability

    VIII. Enzymatic Basis of Repair

    IX. Summary: The Role of Excision Repair

    References

    12 Formation and Repair of Benzo[a]Pyrene-Induced DNA Damage in Mammalian Cells

    I. Introduction

    II. Formation of Covalent Benzo[a]pyrene-DNA Adducts in Mammalian Cells

    III. Repair of Benzo[<2]pyrene-DNA Adducts

    IV. Indirect Formation of DNA-Base Damage by Reactive Oxygen Radical Species Produced as a Consequence of Benzo[a]pyrene Metabolism

    References

    13 Repair of Human DNA Damaged by Ultraviolet and Benzo[a]Pyrene Diol Epoxide I

    Text

    References

    14 Effect of DNA Repair on the Cytotoxicity and Mutagenicity of Polycyclic Hydrocarbon Metabolites in Human Cells

    I. Introduction

    II. Experimental Procedures

    III. Results

    IV. Discussion

    References

    Part V Transformation! Cell Culture

    15 Mechanistic Studies of Neoplastic Transformation of Cells in Culture

    I. Introduction

    II. In Vitro Systems for Mechanistic Study of Neoplastic Transformation

    III. Phenotypic Alterations Associated with Neoplastic Cells

    IV. Basic Issues in the Studies of in Vitro Neoplastic Transformation

    V. Areas for Future Research

    References

    16 Oncogenic Transformation of Cell Cultures by Polycyclic Aromatic Hydrocarbons and Their Derivatives

    Text

    References

    17 Biological Activity of Polycyclic Hydrocarbons in Syrian Hamster Cells In Vitro

    Text

    References

    18 Factors Affecting Polycyclic Hydrocarbon-Induced Cell Transformation

    I. Introduction

    II. Results and Discussion

    III. Conclusion

    References

    Part VI Animal and Human Models

    19 In Vivo and In Vitro Studies of the Evolution of Epithelial Neoplasia

    I. Introduction: The Hypothesis of Neoplastic Development

    II. An Experimental Model for the Study of Incipient Neoplasia

    III. In Vivo-In Vitro Studies with the Explant-Outgrowth System

    IV. In Vivo-In Vitro Studies with Dispersed Epithelial Cells

    V. Discussion: Altered in Vitro Growth Capacity, a Common Feature of Carcinogen-Exposed Cells

    References

    20 Metabolism of Benzo[a]Pyrene in Cultured Human Tissues and Cells

    I. Introduction

    II. Metabolism of Benzo[a]Pyrene

    III. Conclusion

    References

    Part VII Genetics

    21 The Ah Locus: Genetic Regulation of the Enzymes which Metabolize Polycyclic Hydrocarbons and the Risk for Cancer

    I. Introduction and Scope

    II. The Cytochrome Ñ-450 Monooxygenase Systems and Coordinated Enzymes

    III. Genetic Differences in the Risk for Cancer

    IV. "Pleiotypic Response" of the Ah Locus

    V. Evidence for the Human Ah Locus

    VI. Summary

    References

    22 Genetics of Aryl Hydrocarbon Hydroxylase in the Human Population and Its Relationship to Lung Cancer

    I. Introduction

    II. Methods

    III. Results and Discussion

    IV. Summary

    References

    23 Evidence for Linkage between Aryl Hydrocarbon Hydroxylase Expression and Enzyme Markers Assigned to Human Chromosome 2 in Human x Mouse Hybrid Cells

    I. Introduction

    II. Materials and Methods

    III. Results

    IV. Discussion

    V. Summary

    References

    Part VIII Comments and Observations

    24 Polycyclic Hydrocarbon Carcinogenesis: An Overall View

    Text

    Appendix

    References

    25 Comments on Some Biomedical, Environmental, and Societal Aspects

    I. Biomedical Aspects

    II. Environmental Considerations

    III. Societal Aspects

    26 On the Establishment of Biomedical Risk Assessment Systems

    Text

    Subject Index

Product details

  • No. of pages: 473
  • Language: English
  • Copyright: © Academic Press 1978
  • Published: January 1, 1978
  • Imprint: Academic Press
  • eBook ISBN: 9780323149068

About the Editor

Harry Gelboin

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