Mitosis and Meiosis Part B

Mitosis and Meiosis Part B

1st Edition - June 25, 2018

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  • Editors: Helder Maiato, Melina Schuh
  • Hardcover ISBN: 9780128141427
  • eBook ISBN: 9780128141434

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Mitosis and Meiosis, Part B, Volume 145, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, the characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more.

Key Features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics


Researchers and professionals in Cell Biology field

Table of Contents

  • Preface
    Helder Maiato and Melina Schuh
    1. Mitotic live-cell imaging at different timescales
    Anna-Maria Olziersky, Chris A. Smith, Nigel Burroughs, Andrew D. McAinsh and Patrick Meraldi
    2. Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture
    Nicholas I. Clarke and Stephen J. Royle
    3. Quantification of three-dimensional spindle architecture
    Norbert Lindow, Stefanie Redemann, Florian Brünig, Gunar Fabig, Thomas Müller-Reichert and Steffen Prohaska
    4. Quantitative live and super-resolution microscopy of mitotic chromosomes
    Nike Walther and Jan Ellenberg
    5. Live imaging of cell division in 3D stem-cell organoid cultures
    Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen, René M. Overmeer, Hugo J. Snippert and Geert J.P.L. Kops
    6. Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide
    Iskra Yanakieva, Marija Matejcic and Caren Norden
    7. Advanced microscopy methods for bioimaging of mitotic microtubules in plants
    Petra Vyplelová, Miroslav Ovečka, George Komis and Jozef Šamaj
    8. Microsurgery and microinjection techniques in mitosis research
    Charles A. Day, Jessica Hornick, Alyssa Langfald, Christopher Mader and Edward H. Hinchcliffe
    9. Analyzing the micromechanics of the cell division apparatus
    Yuta Shimamoto and Tarun M. Kapoor
    10. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells
    Ana Milas, Mihaela Jagrić, Jelena Martinčić and Iva M. Tolić
    11. Live imaging of C. elegans oocytes and early embryos
    Kimberley Laband, Benjamin Lacroix, Frances Edwards, Julie C. Canman and Julien Dumont
    12. Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes
    Pierre Romé and Hiroyuki Ohkura
    13. Methods to study meiosis in insect spermatocytes
    James R. LaFountain, Jr and Christopher S. Cohan
    14. Live-cell microscopy of meiosis in spermatocytes
    Hiroki Shibuya and Yoshinori Watanabe
    15. Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy
    Judith Reichmann, Manuel Eguren, Yu Lin, Isabell Schneider and Jan Ellenberg
    16. Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos
    Mariia Burdyniuk, Natalia Wesolowska, Michal Fleszar, Matthia A. Karreman, Pedro Machado, Joana Borrego-Pinto, Bernhard Ruthensteiner, Yannick Schwab and Péter Lénárt
    17. A microscopy-based approach for studying meiosis in live and fixed human oocytes
    Agata P. Zielinska and Melina Schuh
    18. Superresolution imaging of the synaptonemal complex
    Katharina Schücker, Markus Sauer and Ricardo Benavente
    19. Cryoelectron tomography of SYCP3 fibres under native conditions
    Daniel Bollschweiler, Laura Radu and Luca Pellegrini

Product details

  • No. of pages: 390
  • Language: English
  • Copyright: © Academic Press 2018
  • Published: June 25, 2018
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128141427
  • eBook ISBN: 9780128141434

About the Serial Volume Editors

Helder Maiato

Helder Maiato graduated in Biochemistry and holds a PhD in Biomedical Sciences from the University of Porto. He was a visiting PhD student at the University of Edinburgh (UK) and a Post-doctoral Research Affiliate at the New York State Department of Health (USA). At present, he is a Principal Investigator at the Institute for Research and Innovation in Health (i3S) and Invited Professor at the Faculty of Medicine of the University of Porto (Portugal). In 2015 he was honoured with the Young Investigator Award from the Louis-Jeantet Foundation and was recently elected EMBO Member. He served as National Counsellor for Science and Technology to the Portuguese Prime Minister and founded Yscience (, a pilot non-profit initiative that brings together scientists to promote science education in young children. His main research interest is the spatiotemporal control of chromosome segregation during cell division.

Affiliations and Expertise

Principal Investigator/Group Leader, Chromosome Instability and Dynamics Lab, Instituto de Investigacao e Inovacao em Saude da Universidade do Porto Rua Alfredo Allen, Porto, Portugal

Melina Schuh

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

Affiliations and Expertise

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Gottingen, Germany

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