Description

This volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.

Key Features

Iincludes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton.
The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.

Readership

cell biologists, biophysicists and pharmacologists as well as for researchers in the biomedical and biotechnology communities.

Table of Contents

  1. Microtubule dynamics at the cell cortex probed by TIRF microscopy
  2. Ilya Grigoriev and Anna Akhmanova

  3. Microscopy methods for the study of centriole biogenesis and function in Drosophila
  4. Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias

  5. Quality control in single molecule studies of kinesins and microtubule-associated proteins
  6. Gary J. Brouhard

  7. Imaging cilia in zebrafish
  8. Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine

  9. Automatic Identification of Microtubules in Cellular Electron Tomography
  10. Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman

  11. Imaging and analysis of the microtubule cytoskeleton in Giardia
  12. Scott C. Dawson and Susan A. House

  13. Kinetochore-microtubule dynamics and attachment stability
  14. Jennifer G. DeLuca

  15. Assessment of mitotic spindle phenotypes in Drosophila S2 cells
  16. Gohta Goshima

  17. Live cell imaging techniques for analyses of microtubules in Dictyostelium
  18. Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf

  19. Microtubule dynamics in dendritic spines
  20. Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad

  21. Analysis of Microtubules in Budding Yeast
  22. Alexander Rauch, Elena Nazarova and Jackie Vogel

  23. Determination of Microtubule Dynamic Instability in Living Cells
  24. Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan

  25. Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos
  26. Akatsuki Kimura1 and Shuichi Onami

  27. Cryo electron tomography of cellular microtubules
  28. Roman I. Koning

  29. Microtubule dynamics in plan

Details

No. of pages:
552
Language:
English
Copyright:
© 2010
Published:
Imprint:
Academic Press
Print ISBN:
9780123813497
Electronic ISBN:
9780123813503