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Section 1 Micropatterning isolated cell components
1 Mitotic Spindle Assembly on Chromatin Patterns Made with Deep UV Photochemistry
Katarzyna Tarnawska, Céline Pugieux and François Nédélec
2 Geometrical control of actin assembly and contractility
Anne-Cécile Reymann, Christophe Guérin, Manuel Théry, Laurent Blanchoin, and Rajaa Boujemaa-Paterski
3 Micropatterning Microtubules
4 Micropatterned, Multicomponent Supported Lipid Bilayers for Cellular Systems
Debjit Dutta and Lance C. Kam
5 Reconstituting functional microtubule-barrier interactions
Núria Taberner, Georges Weber, Changjiang You, Roland Dries, Jacob Piehler and Marileen Dogterom
Section 2 Dynamic micro-patterning
6 Polyacrylamide hydrogel micropatterning
Timothée Vignaud, Hajer Ennomani and Manuel Théry
7 Dynamic Photo-chemical Silane Micro-patterning
8 Dynamic Photo-chemical Lipid Micropatterning for Manipulation of Non-adherent Mammalian Cells
Shinya Yamahira, Yumi Takasaki, Satoshi Yamaguchi, Kimio Sumaru, Toshiyuki Kanamori and Teruyuki Nagamune
9 Thermosensitive micropatterned substrates
Lionel Bureau and Martial Balland
10 Fabrication of Micropatterned Arrays of Gold Nanoparticles for Photothermal Manipulation of Living Cells
Julien Polleux and Guillaume Baffou
11 A reagent-based dynamic trigger for cell adhesion, shape change or cocultures
Stijn F. M. van Dongen, Paolo Maiuri and Matthieu Piel
12 Cell patterning by micro-pattern projection of UV light through photo-induced enhancement of cell adhesion (PIECA)
Kimio Sumaru and Toshiyuki Kanamori
Section 3 Micropatterning for specific applications, from forces to yeats
13 Preparing substrates encoding cell patterning and localized intracellular magnetic particle stimulus for high-throughput experimentation
Peter Tseng and Dino Di Carlo
14 MICROFABRICATED CHAMBERS AS FORCE-SENSORS FOR PROBING FORCES OF FUNGAL GROWTH
15 Visualizing single rod-shaped fission yeast vertically in micro-sized holes on agarose pad made by soft-lithography
Li Wang and Phong T. Tran
16 Microfabricated environments to study collective cell behaviors
Sri Ram Krishna Vedula, Andrea Ravasio, Ester Anon, Tianchi Chen, Grégoire Peyret, Mohammed Ashraf and Benoit Ladoux
This new volume of Methods in Cell Biology is the second volume describing micropatterning, complementing Volume 120. Chapters are written by experts in the field and include cutting-edge material.
- Includes sections on micropatterning in 2D with photomask, maskless micropatterning and 2D nanopatterning
- Chapters are written by experts in the field
- Cutting-edge material
Researchers and students in cell, molecular and developmental biology
- No. of pages:
- © Academic Press 2014
- 27th January 2014
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development.
Systems Biology of Cell Division and Cell Polarity, Cell Biology and Cancer Department, Institut Curie, Paris, France Institut Pierre Gilles de Gennes for Microfluidics, Paris, France
Physics of the cytoskeleton and morphogenesis, LPCV / iRTSV / DSV / CEA, France
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