Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

1st Edition - November 22, 2018
  • Editors: Matthieu Piel, Daniel Fletcher, Junsang Doh
  • eBook ISBN: 9780128142851
  • Hardcover ISBN: 9780128142844

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Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more.

Key Features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics


Researchers and professors in the cell biology field

Table of Contents

  • Section 1 Microfluidics for cell culture, cell manipulation and cell sorting
    1. Viable cell culture in PDMS-based microfluidic devices
    Melikhan Tanyeri and Savas Tay
    2. High-throughput microfluidic single-cell trapping arrays for biomolecular and imaging analysis
    Xuan Li and Abraham P. Lee
    3. Artificial niche microarrays for identifying extrinsic cell-fate determinants
    Samy Gobaa, Raphael V. Gayet and Matthias P. Lutolf
    4. Reconstruction of directed neuronal networks in a microfluidic device with asymmetric microchannels
    Josquin Courte, Renaud Renault, Audric Jan, Jean-Louis Viovy, Jean-Michel Peyrin and Catherine Villard
    5. Single-cell 3D electro-rotation
    Liang Huang, Peng Zhao, Fei Liang and Wenhui Wang

    Section 2 Droplet-based microfluidics
    6. Direct quantification of EGFR variant allele frequency in cell-free DNA using a microfluidic-free digital droplet PCR assay
    Benjamin Demaree, Daniel Weisgerber, Ata Dolatmoradi, Makiko Hatori and Adam R. Abate
    7. Quantifying phenotypes in single cells using droplet microfluidics
    Fengjiao Lyu, Lucas R. Blauch and Sindy K.Y. Tang
    8. Modular microfluidics for double emulsion formation
    Bryant Thompson, Nareh Movsesian, Christine Cheng, Prathamesh Karandikar, Malancha Gupta and Noah Malmstadt
    9. Universal anchored-droplet device for cellular bioassays
    Gabriel Amselem, Sébastien Sart and Charles N. Baroud

    Section 3 Microfluidics for cellular analysis
    10. Cell biology at the interface of nanobiosensors and microfluidics
    Nikhil Bhalla, Hung-Ju Chiang and Amy Q. Shen
    11. BET-seq: Binding energy topographies revealed by microfluidics and high-throughput sequencing
    Arjun K. Aditham, Tyler C. Shimko and Polly M. Fordyce

Product details

  • No. of pages: 264
  • Language: English
  • Copyright: © Academic Press 2018
  • Published: November 22, 2018
  • Imprint: Academic Press
  • eBook ISBN: 9780128142851
  • Hardcover ISBN: 9780128142844

About the Serial Volume Editors

Matthieu Piel

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development.

Affiliations and Expertise

Systems Biology of Cell Division and Cell Polarity, Cell Biology and Cancer Department, Institut Curie, Paris, France Institut Pierre Gilles de Gennes for Microfluidics, Paris, France

Daniel Fletcher

Dr. Fletcher and his team develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. His lab specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of his work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases.

Affiliations and Expertise

Purnendu Chatterjee Chair in Engineering Biological Systems, Department of Bioengineering, University of California Berkeley; Scientist and Deputy Division Director, Physical Biosciences Division, Lawrence Berkeley National Laboratory

Junsang Doh

Junsang Doh is an associate professor of Mechanical Engineering/Interdisciplinary Bioscience and Bioengineering (I-Bio) in POSTECH, South Korea. Prof. Doh’s group develops and utilizes engineering tools such as microfabrication/imaging/mechanics to study fundamental aspects of immune cell behaviors, including synapse-based cell-cell interactions and motility under complex microenvironments, in the context of cancer immunotherapy.

Affiliations and Expertise

Associate Professor, Department of Mechanical Engineering, School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology