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Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others.
This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields.
- Offers basic understanding and a clear picture of various MCFs
- Explains several tools and technologies, including DNA synthesis, synthetic biology tools, genome editing, biosensors, computer-aided design, and OMICS tools, among others
- Harnesses the potential of engineered MCFs to produce a wide range of biomolecules for industrial, therapeutic, pharmaceutical, nutraceutical and biotechnological applications
- Highlights the advances, challenges, and future opportunities in designing MCFs
Researchers in field of metabolic engineering, genome engineering, synthetic biology and industrial microbiology
1. Advances and opportunity in design of microbial cell factories for production of biomolecules
2. Advances in long DNA synthesis
3. Discovery of enzymes responsible for cyclization and post-modification in triterpenoid biosynthesis
4. Synthetic biology for metabolic engineering of secondary metabolites
5. Synthetic biology design tools for metabolic engineering
6. Metabolic engineering for microbial cell factories
7. CRISPR based tools for microbial cell factories
8. Escherichia coli, the workhorse cell factory for the production of chemicals
9. Bacillus subtilis based microbial cell factories
10. Pseudomonas putida- based cell factories
11. Streptomyces based cell factories for production of biomolecules and bioactive metabolites
12. Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: Fundamentals and applications
13. Production of high value-added chemicals in engineering methylotrophic cell factories
14. Cyanobacteria based microbial cell factories for production of industrial products
15. Integrated omics perspective to understand the production of high-value added biomolecules (HVABs) in microalgal cell factories
16. Saccharomyces cerevisiae as a microbial cell factory
17. Pichia pastoris based microbial cell factories
18. Yarrowia lipolytica engineering as a source of microbial cell factories
19. Engineering of microbial cell factories for production of plant-based natural products
20. Engineering of microbial cell factories for Omic-guided production of medically important biomolecules
21. Advances and applications of cell-free systems for metabolic production
22. Microbial biosensors for discovery and engineering of enzymes and metabolism
23. Manipulation of global regulators in Escherichia coli for the synthesis of biotechnologically relevant products
- No. of pages:
- © Academic Press 2021
- 13th February 2021
- Academic Press
- eBook ISBN:
- Paperback ISBN:
Dr. Vijai Singh is an Associate Professor and Dean (Research & Innovation) School of Science at Indrashil University, Rajpur, Mehsana, Gujarat, India. He was an Assistant Professor in the Department of Biological Sciences and Biotechnology at Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology (2009) from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India. During his PhD, he has cloned, expressed and characterized hemolysin from Aeromonas hydrophila in Escherichia coli. He has extensive experience in Synthetic Biology including MAGE, small regulatory RNAs, pathway designing, CRISPR-Cas systems, and microfluidics. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 9 years of research and teaching experience in synthetic biology, metabolic engineering, microbiology, and industrial microbiology. He has published 76 articles, 40 chapters, 10 books and 2 patents. He serves as an associate editor, editorial board member and reviewer of a number of peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of Institutional Biosafety Committee (IBSC) in the same University.
Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India
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