Methods of Hormone Radioimmunoassay

List of Contributors

Preface

Preface to First Edition

Intracellular Messengers


1 Cyclic AMP and Cyclic GMP

I. Introduction

II. Method of Radioimmunoassay

III. Problems Related to Measuring cAMP and cGMP

References


2 Prostaglandins and Prostaglandin Metabolites

I. Introduction

II. Method of Radioimmunoassay

III. Evaluation of the Radioimmunoassay

IV. Prostaglandin Metabolites

References

Hypothalamic and Pineal Hormones


3 Thyrotropin-Releasing Hormone

I. Introduction

II. Method of Radioimmunoassay

III. Assay and Inactivation of TRH in Biologic Fluids and Tissue

IV. Conclusions

References


4 Gonadotropin-Releasing Hormone

I. Introduction

II. Radioimmunoassay

III. Summary

References


5 Somatostatin

I. Introduction

II. Materials

III. Methods

IV. Characteristics of the SRIF Radioimmunoassay

V. Measurement of SRIF

References


6 Melatonin

I. Introduction

II. Methods of Radioimmunoassay

III. Radioimmunoassay Technique

IV. Applications and Importance of Melatonin Radioimmunoassay

V. Radioiodinated Melatonin Analog

References


7 Substance P

I. Introduction

II. Assay Techniques

III. Some Characteristics and Results of the Assay

References


8 Neurotensin and Related Substances

I. Introduction

II. General Considerations for Intermediate-Sized Peptides

III. Specific Considerations for Neurotensin

IV. Methods

V. Conclusion

References

Pituitary Hormones


9 Pituitary Gonadotropins

I. Introduction

II. Procedural Details of Radioimmunoassay

III. Application to Measurement of Tissue Receptor-Bound LH

IV Normal Values for Human LH and FSH

V. Conclusions

References


10 Prolactin

I. Introduction

II. Bioassays

III. Radioimmunoassays

IV. Radioimmunoassay Method

V. Hormonal Heterogeneity

VI. Clinical Results

References


11 Growth Hormone

I. Introduction

II. Preparation of Buffers and Columns for hGH Assay

III. Technique of Radioimmunoassay

IV. Cross-Reactivity with Other Hormones

V. Measurements of the Hormone in Blood

VI. Measurements of the Hormone in Tissue and Urine

References


12 Adrenocorticotropic Hormone (ACTH)

I. Introduction

II. Methods of Radioimmunoassay

III. Problems

IV. Other Radioimmunoassays

V Recent Developments

References


13 Melanocyte-Stimulating Hormones (MSH's) and Lipotropic Hormones (LPH's)

I. Introduction

II. Methods of Radioimmunoassay

III. Problems

References


14 Thyrotropin

I. Introduction

II. Method of Radioimmunoassay

III. Other Immunoassays

References


15 Oxytocin

I. Introduction and History

II. Methods of Radioimmunoassay

III. Sensitivity, Cross-Reactivity, and Validation

IV Oxytocin in Plasma

V. Discussion

References


16 Vasopressin

I. Introduction and History

II. Methods of Radioimmunoassay

III. Sensitivity, Cross-Reactivity, and Validation

IV. Vasopressin in Plasma

V. Comparison with Other Assays

References

Thyroid and Parathyroid Hormones


17 Human Calcitonin: Application of Affinity Chromatography

I. Introduction

II. Method of Radioimmunoassay

III. Interpretation of Radioimmunoassay Data

IV. Concentrations of Calcitonin in Human Serum and Urine

V. Affinity Chromatography

References


18 Thyroxine and Triiodothyronine

I. Introduction

II. Principles of T3 and T4 Radioimmunoassay

III. Rapid Simultaneous Radioimmunoassay for T3 and T4 in Unextracted Serum

IV. Comparison of Various T3 and T4 Radioimmunoassay Techniques and Results

V. Physiological Considerations

VI. Screening for Congenital Hypothyroidism

VII. Summary and Conclusions

References


19 Parathyroid Hormone

I. Introduction

II. Methodology

III. Interpretation of Radioimmunoassay Data

References

Renal Hormones


20 Erythropoietin

I. Introduction

II. Erythropoietin Sources

III. Radioimmunoassay

IV. Measurements of Erythropoietin in Blood

V Final Comments

References


21 Vitamin D Metabolites

I. Introduction

II. 25-OHD Assays

III. 1,25-(OH)2D Assays

IV. 24,25-(OH)2D Assays

V. Summary

References

Addendum

Hormones of the Gastrointestinal Tract


22 Gastrin and Related Peptides

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation of Radioimmunoassay Data

IV. Measurement of Gastrin Tetrapeptide

References


23 Secretin

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation and Validation of the Method

References


24 Cholecystokinin-Pancreozymin

I. Introduction

II. Methods

III. Evaluation of the Radioimmunoassay

IV. Results Obtained with Cholecystokinin Radioimmunoassays

References


25 Serotonin

I. Introduction

II. Methods of Radioimmunoassay

III. Validation and Characterization of the Assay

IV. Summary

References


26 Gastric Inhibitory Polypeptide

I. Introduction

II. Method of Radioimmunoassay

III. Evaluation of Radioimmunoassay Data

References


27 Vasoactive Intestinal Peptide

I. Introduction

II. Method of Radioimmunoassay

III. Assay Characteristics

IV. Conclusion

References


28 Motilin

I. Introduction

II. Method of Radioimmunoassay

III. Evaluation of the Radioimmunoassay

IV Affinity Chromatography

References


29 Bombesin-like Peptides

I. Introduction

II. Method of Radioimmunoassay

III. Bombesin-like Material in Tissues

References


30 Bile Acids

I. Introduction

II. Methods of Radioimmunoassay

III. Vilidation of Assay

IV Normal Values and Concentrations of the Individual Bile Acids in Serum in Various States of Hepatobiliary Disease

References

Pancreatic Hormones


31 Insulin, Proinsulin, and C-Peptide

I. Introduction

II. Methods of Radioimmunoassay

III. Verification of Assay Data

IV Problems Related to the Measurement of Insulin and Proinsulin

V. Other Radioimmunoassays Available

VI. Radioimmunoassay for Human C-Peptide

References


32 Glucagon

I. Introduction

II. Special Problems Encountered in the Measurement of Plasma Glucagon by Radioimmunoassay

III. Methods of Radioimmunoassay

References


33 Human Pancreatic Polypeptide (HPP) and Bovine Pancreatic Polypeptide (BPP)

I. Introduction

II. Methods of Radioimmunoassay

III. Homologous and Heterologous Assays

IV. Alternate Methods

References

Steroid Hormones


34 Plasma Estradiol, Estrone, Estriol, and Urinary Estriol Glucuronide

I. Plasma Estradiol, Estrone, and Estriol

II. Urinary Estriol Glucuronide

References


35 Progesterone and 20a-Dihydroprogesterone

I. Introduction

II. Methods of Radioimmunoassay

III. Sensitivity and Precision

References


36 Androgens: Testosterone and Dihydrotestosterone

I. Introduction

II. Method of Radioimmunoassay

III. Validation of Assay

IV. Normal Values

V. Other Androgens—Ahdrostenedione

References


37 Radioiodinated Steroid Hormones—General Principles

I. Introduction

II. Advantages and Disadvantages of Radioiodinated Steroids

III. Preparation of the Steroid Analogs for Radioiodination

IV. Radioiodination of Steroid Hormones

V. Purification of Radioiodinated Steroid Hormones

VI. Development and/or Adaptation of Radioimmunoassay Procedures

VII. Summary

References


38 Mineralocorticoids: Aldosterone, Deoxycorticosterone, 18-Hydroxydeoxycorticosterone, and 18-Hydroxycorticosterone

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation of the Radioimmunoassay

IV. Problems Related to Measuring Mineralocorticoids

V. Other Radioimmunoassay Methods

VI. New Developments

References


39 Glucocorticoids: Cortisol, Cortisone, Corticosterone, Compound S, and Their Metabolites

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation of Radioimmunoassay Data

IV. Other Uses for Cortisol and Corticosterone Antibody

V. General Discussion

References


40 Arthropod Molting Hormones

I. Introduction

II. Quantification of Ecdysteroids

III. Comparison of Available Radioimmunoassays for Ecdysteroids

IV. Protocol

V. Dual Antisera Determinations

References

Uterine and Placental Hormones


41 Specific Human Chorionic Gonadotropin Assay

I. Introduction

II. Method of Radioimmunoassay

References


42 Radioimmuno- and Radioreceptor Assays of Placental Lactogens

I. Introduction

II. Radioimmunoassay

III. Radioreceptor Assays for hPL

IV. Applications of Radioimmunoassay and Radioreceptor Assays

V. Summary

References


43 Radioimmunoassay of Human Chorionic Thyrotropin

I. Introduction

II. Technique of hCT Radioimmunoassay

III. Results and Interpretation

References


44 Relaxin

I. Introduction

II. Method of Radioimmunoassay

III. Radioimmunoassay Characterization

IV. Levels of Circulating Relaxin

References

Vasoactive Peptide Hormones


45 Renin and the Angiotensins

I. Introduction

II. Methods of Radioimmunoassay

III. Verification of Assay Results and Special Problems

IV. Commercial Kits and Concluding Comments

References


46 Bradykinin

I. Introduction

II. Radioimmunoassay Methodology

III. Validation and Standards

References


47 Urogastrone—Epidermal Growth Factor

I. Introduction

II. Methods of Radioimmunoassay

III. Urogastrone Measurements

References


48 Nerve Growth Factor: Radioimmunoassay and Bacteriophage Immunoassay

I. Background Information

II. Radioimmunoassay

III. Bacteriophage Immunoassay

IV. Measurement of NGF in Cells and Biologic Fluids

V. Summary

References


49 Somatomedin A, Somatomedin C, and NSILA-s

I. Introduction

II. Preparation of Buffers and Columns for the Somatomedins A and C Assays

III. Preparation of Placental Membranes

IV. Technique of Radioreceptor Assay for Somatomedins A and C

V. Preparation of Buffer and Column for the NSILA-s Assay

VI. Technique of Radioreceptor Assay for NSILA-s

VII. Cross-Reactivity with Other Growth Factors

VIII. Measurement of Somatomedins A and C and NSILA-s in Blood

References

Appendixes

I. Summary of Radioimmunoassay Methodology

II. Commercial Sources of Radioimmunoassay Components

III. Normal Values As Determined by Radioimmunoassay

Index