Methods of Hormone Radioimmunoassay

Methods of Hormone Radioimmunoassay

2nd Edition - January 28, 1979

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  • Editor: Bernard Jaffe
  • eBook ISBN: 9780323161268

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Description

Methods of Hormone Radioimmunoassay, Second Edition is a compilation of radioimmunoassay techniques for the quantification of hormones. Various kinds of hormones are considered, namely: intracellular messengers; hypothalamic and pineal hormones; pituitary hormones; thyroid and parathyroid hormones; renal hormones; hormones of the gastrointestinal tract; pancreatic hormones; steroid hormones; uterine and placental hormones; vasoactive peptide hormones; and growth factors. The corresponding method of radioimmunoassay for each hormone is described. This book is comprised of 49 chapters and begins with a discussion on the radioimmunoassay of cyclic adenosine-3',5-monophosphate (cAMP) and cyclic guanosine-3,5'-monophosphate (cGMP), together with problems related to measuring both compounds. The reader is then introduced to prostaglandins and prostaglandin metabolites, thyrotropin-releasing hormone, and gonadotropin-releasing hormone. Pituitaty gonadotropins, prolactin, and thyrotropin are also discussed. The remaining chapters deal with hormones of the gastrointestinal tract, pancreatic hormones, steroid hormones, and growth factors. This monograph is intended for both clinicians and investigators interested in methods of hormone radioimmunoassay.

Table of Contents


  • List of Contributors

    Preface

    Preface to First Edition

    Intracellular Messengers

    1 Cyclic AMP and Cyclic GMP

    I. Introduction

    II. Method of Radioimmunoassay

    III. Problems Related to Measuring cAMP and cGMP

    References

    2 Prostaglandins and Prostaglandin Metabolites

    I. Introduction

    II. Method of Radioimmunoassay

    III. Evaluation of the Radioimmunoassay

    IV. Prostaglandin Metabolites

    References

    Hypothalamic and Pineal Hormones

    3 Thyrotropin-Releasing Hormone

    I. Introduction

    II. Method of Radioimmunoassay

    III. Assay and Inactivation of TRH in Biologic Fluids and Tissue

    IV. Conclusions

    References

    4 Gonadotropin-Releasing Hormone

    I. Introduction

    II. Radioimmunoassay

    III. Summary

    References

    5 Somatostatin

    I. Introduction

    II. Materials

    III. Methods

    IV. Characteristics of the SRIF Radioimmunoassay

    V. Measurement of SRIF

    References

    6 Melatonin

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Radioimmunoassay Technique

    IV. Applications and Importance of Melatonin Radioimmunoassay

    V. Radioiodinated Melatonin Analog

    References

    7 Substance P

    I. Introduction

    II. Assay Techniques

    III. Some Characteristics and Results of the Assay

    References

    8 Neurotensin and Related Substances

    I. Introduction

    II. General Considerations for Intermediate-Sized Peptides

    III. Specific Considerations for Neurotensin

    IV. Methods

    V. Conclusion

    References

    Pituitary Hormones

    9 Pituitary Gonadotropins

    I. Introduction

    II. Procedural Details of Radioimmunoassay

    III. Application to Measurement of Tissue Receptor-Bound LH

    IV Normal Values for Human LH and FSH

    V. Conclusions

    References

    10 Prolactin

    I. Introduction

    II. Bioassays

    III. Radioimmunoassays

    IV. Radioimmunoassay Method

    V. Hormonal Heterogeneity

    VI. Clinical Results

    References

    11 Growth Hormone

    I. Introduction

    II. Preparation of Buffers and Columns for hGH Assay

    III. Technique of Radioimmunoassay

    IV. Cross-Reactivity with Other Hormones

    V. Measurements of the Hormone in Blood

    VI. Measurements of the Hormone in Tissue and Urine

    References

    12 Adrenocorticotropic Hormone (ACTH)

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Problems

    IV. Other Radioimmunoassays

    V Recent Developments

    References

    13 Melanocyte-Stimulating Hormones (MSH's) and Lipotropic Hormones (LPH's)

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Problems

    References

    14 Thyrotropin

    I. Introduction

    II. Method of Radioimmunoassay

    III. Other Immunoassays

    References

    15 Oxytocin

    I. Introduction and History

    II. Methods of Radioimmunoassay

    III. Sensitivity, Cross-Reactivity, and Validation

    IV Oxytocin in Plasma

    V. Discussion

    References

    16 Vasopressin

    I. Introduction and History

    II. Methods of Radioimmunoassay

    III. Sensitivity, Cross-Reactivity, and Validation

    IV. Vasopressin in Plasma

    V. Comparison with Other Assays

    References

    Thyroid and Parathyroid Hormones

    17 Human Calcitonin: Application of Affinity Chromatography

    I. Introduction

    II. Method of Radioimmunoassay

    III. Interpretation of Radioimmunoassay Data

    IV. Concentrations of Calcitonin in Human Serum and Urine

    V. Affinity Chromatography

    References

    18 Thyroxine and Triiodothyronine

    I. Introduction

    II. Principles of T3 and T4 Radioimmunoassay

    III. Rapid Simultaneous Radioimmunoassay for T3 and T4 in Unextracted Serum

    IV. Comparison of Various T3 and T4 Radioimmunoassay Techniques and Results

    V. Physiological Considerations

    VI. Screening for Congenital Hypothyroidism

    VII. Summary and Conclusions

    References

    19 Parathyroid Hormone

    I. Introduction

    II. Methodology

    III. Interpretation of Radioimmunoassay Data

    References

    Renal Hormones

    20 Erythropoietin

    I. Introduction

    II. Erythropoietin Sources

    III. Radioimmunoassay

    IV. Measurements of Erythropoietin in Blood

    V Final Comments

    References

    21 Vitamin D Metabolites

    I. Introduction

    II. 25-OHD Assays

    III. 1,25-(OH)2D Assays

    IV. 24,25-(OH)2D Assays

    V. Summary

    References

    Addendum

    Hormones of the Gastrointestinal Tract

    22 Gastrin and Related Peptides

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Evaluation of Radioimmunoassay Data

    IV. Measurement of Gastrin Tetrapeptide

    References

    23 Secretin

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Evaluation and Validation of the Method

    References

    24 Cholecystokinin-Pancreozymin

    I. Introduction

    II. Methods

    III. Evaluation of the Radioimmunoassay

    IV. Results Obtained with Cholecystokinin Radioimmunoassays

    References

    25 Serotonin

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Validation and Characterization of the Assay

    IV. Summary

    References

    26 Gastric Inhibitory Polypeptide

    I. Introduction

    II. Method of Radioimmunoassay

    III. Evaluation of Radioimmunoassay Data

    References

    27 Vasoactive Intestinal Peptide

    I. Introduction

    II. Method of Radioimmunoassay

    III. Assay Characteristics

    IV. Conclusion

    References

    28 Motilin

    I. Introduction

    II. Method of Radioimmunoassay

    III. Evaluation of the Radioimmunoassay

    IV Affinity Chromatography

    References

    29 Bombesin-like Peptides

    I. Introduction

    II. Method of Radioimmunoassay

    III. Bombesin-like Material in Tissues

    References

    30 Bile Acids

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Vilidation of Assay

    IV Normal Values and Concentrations of the Individual Bile Acids in Serum in Various States of Hepatobiliary Disease

    References

    Pancreatic Hormones

    31 Insulin, Proinsulin, and C-Peptide

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Verification of Assay Data

    IV Problems Related to the Measurement of Insulin and Proinsulin

    V. Other Radioimmunoassays Available

    VI. Radioimmunoassay for Human C-Peptide

    References

    32 Glucagon

    I. Introduction

    II. Special Problems Encountered in the Measurement of Plasma Glucagon by Radioimmunoassay

    III. Methods of Radioimmunoassay

    References

    33 Human Pancreatic Polypeptide (HPP) and Bovine Pancreatic Polypeptide (BPP)

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Homologous and Heterologous Assays

    IV. Alternate Methods

    References

    Steroid Hormones

    34 Plasma Estradiol, Estrone, Estriol, and Urinary Estriol Glucuronide

    I. Plasma Estradiol, Estrone, and Estriol

    II. Urinary Estriol Glucuronide

    References

    35 Progesterone and 20a-Dihydroprogesterone

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Sensitivity and Precision

    References

    36 Androgens: Testosterone and Dihydrotestosterone

    I. Introduction

    II. Method of Radioimmunoassay

    III. Validation of Assay

    IV. Normal Values

    V. Other Androgens—Ahdrostenedione

    References

    37 Radioiodinated Steroid Hormones—General Principles

    I. Introduction

    II. Advantages and Disadvantages of Radioiodinated Steroids

    III. Preparation of the Steroid Analogs for Radioiodination

    IV. Radioiodination of Steroid Hormones

    V. Purification of Radioiodinated Steroid Hormones

    VI. Development and/or Adaptation of Radioimmunoassay Procedures

    VII. Summary

    References

    38 Mineralocorticoids: Aldosterone, Deoxycorticosterone, 18-Hydroxydeoxycorticosterone, and 18-Hydroxycorticosterone

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Evaluation of the Radioimmunoassay

    IV. Problems Related to Measuring Mineralocorticoids

    V. Other Radioimmunoassay Methods

    VI. New Developments

    References

    39 Glucocorticoids: Cortisol, Cortisone, Corticosterone, Compound S, and Their Metabolites

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Evaluation of Radioimmunoassay Data

    IV. Other Uses for Cortisol and Corticosterone Antibody

    V. General Discussion

    References

    40 Arthropod Molting Hormones

    I. Introduction

    II. Quantification of Ecdysteroids

    III. Comparison of Available Radioimmunoassays for Ecdysteroids

    IV. Protocol

    V. Dual Antisera Determinations

    References

    Uterine and Placental Hormones

    41 Specific Human Chorionic Gonadotropin Assay

    I. Introduction

    II. Method of Radioimmunoassay

    References

    42 Radioimmuno- and Radioreceptor Assays of Placental Lactogens

    I. Introduction

    II. Radioimmunoassay

    III. Radioreceptor Assays for hPL

    IV. Applications of Radioimmunoassay and Radioreceptor Assays

    V. Summary

    References

    43 Radioimmunoassay of Human Chorionic Thyrotropin

    I. Introduction

    II. Technique of hCT Radioimmunoassay

    III. Results and Interpretation

    References

    44 Relaxin

    I. Introduction

    II. Method of Radioimmunoassay

    III. Radioimmunoassay Characterization

    IV. Levels of Circulating Relaxin

    References

    Vasoactive Peptide Hormones

    45 Renin and the Angiotensins

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Verification of Assay Results and Special Problems

    IV. Commercial Kits and Concluding Comments

    References

    46 Bradykinin

    I. Introduction

    II. Radioimmunoassay Methodology

    III. Validation and Standards

    References

    47 Urogastrone—Epidermal Growth Factor

    I. Introduction

    II. Methods of Radioimmunoassay

    III. Urogastrone Measurements

    References

    48 Nerve Growth Factor: Radioimmunoassay and Bacteriophage Immunoassay

    I. Background Information

    II. Radioimmunoassay

    III. Bacteriophage Immunoassay

    IV. Measurement of NGF in Cells and Biologic Fluids

    V. Summary

    References

    49 Somatomedin A, Somatomedin C, and NSILA-s

    I. Introduction

    II. Preparation of Buffers and Columns for the Somatomedins A and C Assays

    III. Preparation of Placental Membranes

    IV. Technique of Radioreceptor Assay for Somatomedins A and C

    V. Preparation of Buffer and Column for the NSILA-s Assay

    VI. Technique of Radioreceptor Assay for NSILA-s

    VII. Cross-Reactivity with Other Growth Factors

    VIII. Measurement of Somatomedins A and C and NSILA-s in Blood

    References

    Appendixes

    I. Summary of Radioimmunoassay Methodology

    II. Commercial Sources of Radioimmunoassay Components

    III. Normal Values As Determined by Radioimmunoassay

    Index

Product details

  • No. of pages: 1070
  • Language: English
  • Copyright: © Academic Press 1979
  • Published: January 28, 1979
  • Imprint: Academic Press
  • eBook ISBN: 9780323161268

About the Editor

Bernard Jaffe

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