Methods of Animal Experimentation

Methods of Animal Experimentation

Volume II

1st Edition - January 1, 1965

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  • Editor: William I. Gay
  • eBook ISBN: 9781483261539

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Methods of Animal Experimentation, Volume II, provides information on the most common methods for using animals as tools in the search for new biological knowledge. The techniques described will facilitate the most efficient use of research animals and provide guidelines for their utmost comfort and welfare. The text is arranged according to specific research methods rather than to organ system or disease category. This approach gives the reader a broad view of the techniques involved in specific fields and describes the range of usefulness of these techniques. The chapters in the present volume deal with special techniques which have been demonstrated to be distinct, useful methods for using laboratory animals as a basic biomedical research tool. The descriptions of both fundamental and well-developed techniques of animal experimentation in various research fields should be useful to graduate students and experienced scientists who must consider variations in research approaches. The book is a source of information for the scientist administrator who is frequently confronted with different proposed approaches to biological research projects utilizing animals.

Table of Contents

  • List of Contributors


    Contents of Volume I


    Chapter 1 Radiation Hygiene

    I. The Nature and Assessment of Ionizing Radiation Effects

    II. Health Physics



    Chapter 2 Use of Ionizing Radiation for Measuring Biological Phenomena

    I. Introduction

    II. Laboratory Design

    III. Handling of Radioisotopes in the Animal Colony

    IV. Administration of Radiation or Radioisotopes to the Experimental Animal

    V. Collection of Samples

    VI. Uses of Radioisotopes



    Chapter 3 Tumor Transplantation

    I. Introduction

    II. History and Biological Properties of 38 Transplantable Mouse, Rat, Hamster, and Chicken Tumors

    III. Techniques of Tumor Transplantation

    IV. Importance of Uniform Inoculum

    V. Importance of Animal Strain

    VI. Inoculation Site

    VII. Influence of Age and Sex

    VIII. Influence of Health

    IX. Influence of Pregnancy

    X. Interval after Which Growth Becomes Apparent

    XI. Comparative Growth Rate of the Malignant Cells

    XII. Inoculation of Stationary or Receding Tumors

    XIII. Spontaneous Regression of Transplantable Tumors

    XIV. Resistance to Reinoculation

    XV. Frozen Storage of Transplantable Tumors

    XVI. Summary


    Chapter 4 Parabiosis

    I. Introduction

    II. Methods

    III. Blood and Fluid Exchange Between Parabiotic Rats

    IV. Diseases of Parabiosis


    Chapter 5 Organ Transplantation

    I. General Discussion of the Types of Problem That Can Be Investigated by Experimental Organ Transplantation

    II. The Selection of Animals for Organ Transplantation

    III. The Surgical Technique

    IV. Methods of Assessment of Renal Function and Homograft Reaction in Animals with Renal Transplants

    V. Summary


    Chapter 6 The Care and Use of Hibernating Mammals

    I. Hibernation

    II. Mammals That Hibernate

    III. Types of Mammalian Hibernation

    IV. Environmental Factors

    V. Hibernation versus Hypothermia

    VI. Estivation

    VII. Choice of Animal for Research

    VIII. Procurement of Animals

    IX. Care of Individual Species

    X. Techniques for Use of Hibernators


    Chapter 7 High-Altitude Studies

    I. Introduction

    II. "Natural" High-Altitude Studies

    III. "Simulated" High-Altitude Studies

    IV. Conclusions


    Chapter 8 Care and Growth of Animals during Chronic Centrifugation

    I. Introduction

    II. Applications

    III. General Considerations in the Design of Centrifuge Programs

    IV. Specific Procedures Employed with Experimental Animals

    V. Procedures of Measurement and Analysis

    VI. Summary


    Chapter 9 Controlled Exercise

    I. Introduction

    II. Methods of Inducing Controlled Exercise

    III. Factors Influencing Controlled Exercise Results

    IV. Factors Affecting Endurance

    VI Influence of Excess Exercise on Various Animal Characteristics

    VI. Summary


    Chapter 10 Methods of Animal Infection with Bacteria, Fungi, and Viruses

    I. Introduction

    II. Animal Inoculation versus Tissue Culture Testing and the Use of Artificial Media

    III. Factors Influencing the Choice of Animals and Route of Inoculation

    IV. General Procedures According to Route of Inoculation

    V. Study of Specific Infections


    Chapter 11 Electronic Monitoring of Physiological Phenomena in Experimental Animals

    I. Components of a Monitoring System

    II. Electrocardiography

    III. Intravascular and Intracardiac Electrocardiography

    IV. Phonocardiography

    V. Intravascular Pressures

    VI. Ultrasound

    VII. Temperature Monitoring

    VIII. Respiration

    IX. Densitometry

    X. Electrophysiological Signals from the Brain


    Author Index

    Subject Index

Product details

  • No. of pages: 624
  • Language: English
  • Copyright: © Academic Press 1965
  • Published: January 1, 1965
  • Imprint: Academic Press
  • eBook ISBN: 9781483261539

About the Editor

William I. Gay

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