Methods of Animal Experimentation - 1st Edition - ISBN: 9781483232218, 9781483261539

Methods of Animal Experimentation

1st Edition

Volume II

Editors: William I. Gay
eBook ISBN: 9781483261539
Imprint: Academic Press
Published Date: 1st January 1965
Page Count: 624
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Methods of Animal Experimentation, Volume II, provides information on the most common methods for using animals as tools in the search for new biological knowledge. The techniques described will facilitate the most efficient use of research animals and provide guidelines for their utmost comfort and welfare. The text is arranged according to specific research methods rather than to organ system or disease category. This approach gives the reader a broad view of the techniques involved in specific fields and describes the range of usefulness of these techniques. The chapters in the present volume deal with special techniques which have been demonstrated to be distinct, useful methods for using laboratory animals as a basic biomedical research tool. The descriptions of both fundamental and well-developed techniques of animal experimentation in various research fields should be useful to graduate students and experienced scientists who must consider variations in research approaches. The book is a source of information for the scientist administrator who is frequently confronted with different proposed approaches to biological research projects utilizing animals.

Table of Contents

List of Contributors


Contents of Volume I


Chapter 1 Radiation Hygiene

I. The Nature and Assessment of Ionizing Radiation Effects

II. Health Physics



Chapter 2 Use of Ionizing Radiation for Measuring Biological Phenomena

I. Introduction

II. Laboratory Design

III. Handling of Radioisotopes in the Animal Colony

IV. Administration of Radiation or Radioisotopes to the Experimental Animal

V. Collection of Samples

VI. Uses of Radioisotopes



Chapter 3 Tumor Transplantation

I. Introduction

II. History and Biological Properties of 38 Transplantable Mouse, Rat, Hamster, and Chicken Tumors

III. Techniques of Tumor Transplantation

IV. Importance of Uniform Inoculum

V. Importance of Animal Strain

VI. Inoculation Site

VII. Influence of Age and Sex

VIII. Influence of Health

IX. Influence of Pregnancy

X. Interval after Which Growth Becomes Apparent

XI. Comparative Growth Rate of the Malignant Cells

XII. Inoculation of Stationary or Receding Tumors

XIII. Spontaneous Regression of Transplantable Tumors

XIV. Resistance to Reinoculation

XV. Frozen Storage of Transplantable Tumors

XVI. Summary


Chapter 4 Parabiosis

I. Introduction

II. Methods

III. Blood and Fluid Exchange Between Parabiotic Rats

IV. Diseases of Parabiosis


Chapter 5 Organ Transplantation

I. General Discussion of the Types of Problem That Can Be Investigated by Experimental Organ Transplantation

II. The Selection of Animals for Organ Transplantation

III. The Surgical Technique

IV. Methods of Assessment of Renal Function and Homograft Reaction in Animals with Renal Transplants

V. Summary


Chapter 6 The Care and Use of Hibernating Mammals

I. Hibernation

II. Mammals That Hibernate

III. Types of Mammalian Hibernation

IV. Environmental Factors

V. Hibernation versus Hypothermia

VI. Estivation

VII. Choice of Animal for Research

VIII. Procurement of Animals

IX. Care of Individual Species

X. Techniques for Use of Hibernators


Chapter 7 High-Altitude Studies

I. Introduction

II. "Natural" High-Altitude Studies

III. "Simulated" High-Altitude Studies

IV. Conclusions


Chapter 8 Care and Growth of Animals during Chronic Centrifugation

I. Introduction

II. Applications

III. General Considerations in the Design of Centrifuge Programs

IV. Specific Procedures Employed with Experimental Animals

V. Procedures of Measurement and Analysis

VI. Summary


Chapter 9 Controlled Exercise

I. Introduction

II. Methods of Inducing Controlled Exercise

III. Factors Influencing Controlled Exercise Results

IV. Factors Affecting Endurance

VI Influence of Excess Exercise on Various Animal Characteristics

VI. Summary


Chapter 10 Methods of Animal Infection with Bacteria, Fungi, and Viruses

I. Introduction

II. Animal Inoculation versus Tissue Culture Testing and the Use of Artificial Media

III. Factors Influencing the Choice of Animals and Route of Inoculation

IV. General Procedures According to Route of Inoculation

V. Study of Specific Infections


Chapter 11 Electronic Monitoring of Physiological Phenomena in Experimental Animals

I. Components of a Monitoring System

II. Electrocardiography

III. Intravascular and Intracardiac Electrocardiography

IV. Phonocardiography

V. Intravascular Pressures

VI. Ultrasound

VII. Temperature Monitoring

VIII. Respiration

IX. Densitometry

X. Electrophysiological Signals from the Brain


Author Index

Subject Index


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© Academic Press 1965
Academic Press
eBook ISBN:

About the Editor

William I. Gay

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