Description

Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.

Key Features

  • The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications
  • Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues
  • The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels

Readership

Investigators and students whose research interests include the development, physiology, or pathophysiology of adipose tissues.

Table of Contents

Contributors

Preface

Chapter One: Adipocyte-Specific Transgenic and Knockout Models

1 Introduction

2 Loss-of-Function Strategies in Adipocytes In Vivo

3 Gain-of-Function Strategies in Adipocytes In Vivo

Chapter Two: Imaging White Adipose Tissue with Confocal Microscopy

1 Introduction

2 Materials for Imaging Adipose Tissue

3 Methods

4 Summary

Acknowledgment

Chapter Three: Isolation and Study of Adipocyte Precursors

1 Introduction

2 Adipose Tissue Depots and Cell Populations

3 Digestion of Whole WAT for Isolation of SVF

4 Flow Cytometry and FACS

5 Primary Adipocyte Precursor Cell Culture and Differentiation

6 Digestion of Whole WAT for Isolation of Mature Adipocytes

Acknowledgment

Chapter Four: Imaging of Adipose Tissue

1 Introduction

2 Imaging of Whole Mounted Adipose Tissue

3 Imaging of Sectioned Adipose Tissue

Acknowledgment

Chapter Five: Adipose Tissue Angiogenesis Assay

1 Introduction

2 Materials

3 Methods

4 Method Limitations

Acknowledgment

Chapter Six: Quantifying Size and Number of Adipocytes in Adipose Tissue

1 Introduction

2 Technical Aspects

3 Future Challenges and Conclusions

Acknowledgment

Chapter Seven: Use of Osmium Tetroxide Staining with Microcomputerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue In Vivo

1 Introduction

2 Materials

3 Methods

4 Summary

Acknowledgment

Chapter Eight: Brown Adipose Tissue in Humans

1 Introduction

2 Positron Emission Tomography

3 Magnetic Resonance Imaging

4 Dual Energy Computed Tomography

Chapter Nine: Analyzing the Functions and Structure of the Human Lipodystrophy Protein Seipin

1 Introduction

2 Technical Aspect

Details

No. of pages:
360
Language:
English
Copyright:
© 2014
Published:
Imprint:
Academic Press
Print ISBN:
9780124116191
Electronic ISBN:
9780124116061