COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Methods in Virology - 1st Edition - ISBN: 9780124702073, 9781483269504

Methods in Virology

1st Edition

Volume VII

Editors: Karl Maramorosch Hilary Koprowski
eBook ISBN: 9781483269504
Imprint: Academic Press
Published Date: 11th October 1984
Page Count: 348
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.


Methods in Virology, Volume VII focuses on the methods used in virology, including radioimmunoassays, microscopy, hybridization, and mutagenesis. The selection first elaborates on monoclonal antibody techniques applied to viruses; competition radioimmunoassays for characterization of antibody reactions to viral antigens; and enzyme immunosorbent assays in plant virology. Discussions focus on the principles of enzyme immunosorbent assay, choice of enzyme and preparation of conjugate, determination of immunoglobulin class, and maintenance and specificity testing of hybridomas. The text then elaborates on electron microscopy for the identification of plant viruses in in vitro preparations and cloning and expression of viral antigens in Escherichia coli and other microorganisms, including influenza virus, expression of foreign coding sequences in Escherichia coli, hepatitis B virus, electron microscope, immunoelectron microscopy, and imaging of nucleic acids. The manuscript takes a look at the detection and characterization of subgenomic RNA in plant viruses; exploring the gene organization of baculoviruses; and spot hybridization for detection of viroids and viruses. Topics include application to viral diseases, mapping mutuations of baculoviruses, transcriptional mapping of baculovirus genomes, and genetic mapping by blot hybridization. The selection is a valuable source of information for researchers interested in the methods employed in virology.

Table of Contents



Contents of Previous Volumes

Chapter 1. Monoclonal Antibody Techniques Applied to Viruses

I. Introduction

II. Immunization Protocol

III. Preparation of Cells

IV. Hybridization

V. Maintenance and Specificity Testing of Hybridomas

VI. Cloning of Hybridomas

VII. Determination of Immunoglobulin Class

VIII. Preparation of Ascites Fluid

IX. Concentration and Purification of Immunoglobulins

X. Characterization of Viral Antigens Using Monoclonal Antibodies

XI. Prospectus

XII. Appendix: Preparation of Reagents


Chapter 2. Competition Radioimmunoassays for Characterization of Antibody Reactions to Viral Antigens

I. Introduction

II. Preparation and Characterization of Reagents

III. Basic Methodologies

IV. Applicability to Identification of Antibody Populations

V. Applicability to Characterization of Viral Antigens

VI. Biological Tracking of Viral Proteins

VII. Concluding Comments


Chapter 3. Enzyme Immunosorbent Assays in Plant Virology

I. Introduction

II. Principles of Enzyme Immunosorbent Assay

III. Preparation of Sample

IV. Production of Antiserum

V. Choice of Enzyme and Preparation of Conjugate

VI. Substrates

VII. Choice of Solid Phase

VIII. Types of ELISA Procedure

IX. Evaluation of Reagents and Analysis of Results

X. Conclusions

XI. Appendix: Buffer Formulations


Chapter 4. Electron Microscopy for the Identification of Plant Viruses in in Vitro Preparations

I. Introduction

II. The Electron Microscope

III. Support Films

IV. Calibration of Magnification

V. Negative Staining

VI. Metal Shadowing

VII. Immunoelectron Microscopy

VIII. Imaging of Nucleic Acids


Chapter 5. Cloning and Expression of Viral Antigens in Escherichia coli and Other Microorganisms

I. Introduction

II. Expression of Foreign Coding Sequences in Escherichia coli

III. Hepatitis B Virus

IV. Foot-and-Mouth Disease Virus

V. Poliovirus: Purification of Polio RNA and Cloning of Poliovirus cDNA

VI. Influenza Virus

VII. Rabies Virus

VIII. Expression of Viral Antigens in Bacillus subtilis

IX. Expression of Viral Antigens in Saccharomyces cerevisiae

X. Conclusion


Chapter 6. Spot Hybridization for Detection of Viroids and Viruses

I. Introduction

II. PSTV Diagnosis by Nucleic Acid Spot Hybridization

III. Application to Other Viroid Diseases

IV. Application to Viral Diseases

V. Supplementary Methodologies


Chapter 7. Detection of Viral Nucleic Acids by in Situ Hybridization

I. Introduction

II. Materials and Methods

III. Applications


Chapter 8. Exploring the Gene Organization of Baculoviruses

I. Introduction

II. Establishing the Physical Map of a Baculovirus

III. Mapping Mutations of Baculoviruses

IV. Correlating Proteins with the Physical Map

V. Transcriptional Mapping of Baculoviruses Genomes

VI. DNA Sequence Homology to Facilitate Mapping Baculoviruses Other than AcNPV

VII. Site-Directed Mutagenesis and Allelic Replacement

VIII. Summary

IX. Addendum


Chapter 9. Detection and Characterization of Subgenomic RNAs in Plant Viruses

I. Introduction

II. Detection and Characterization of Encapsidated Viral Subgenomic RNAs

III. Detection and Characterization of Subgenomic Viral RNAs in Extracts of Infected Tissues

IV. Genetic Mapping by Blot Hybridization

V. Concluding Remarks




No. of pages:
© Academic Press 1984
11th October 1984
Academic Press
eBook ISBN:

About the Editors

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

Hilary Koprowski

Ratings and Reviews