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1. Recombinant tau expression and purification
2. In vitro MT dynamics and MT ends
3. Methods related to investigating tau structure and MT bundling
Cyrus R. Safinya
4. Neurite outgrowth and retraction
5. Methods related to studying tau fragmentation
6. Isoform-specific differences in tau in vitro aggregation
Truman C. Gamblin
7. Cosedimentation assays of microtubule binding
8. Methods to study tau aggregation
9. Methods to study tau phosphorylation
10. Structure of tau oligomers
Nicholas M. Kanaan
11. Use of mouse models for studies of pathological tau action
Virginia Lee and Sneha Narasimhan
12. Application of FRET and FCS to study tau structure-function
13. Use of the fly system to study tau action
14. Single molecule assays with motor proteins
15. Tau clearance mechanisms in primary neurons with a focus on autophagy
16. Structural studies of tau
17. TIRF assays of tau effects on MT and actin cytoskeletons in vitro
18. TIRF assays of tau interactions with EB1
19. A novel approach to address tau biology, pathology, and propagation in vivo in mice using Adeno associated viruses
Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation.
- Covers sections on Tau Cell Biology
- Written by experts in the field of cell biology
- Includes cutting-edge materials
Researchers and students in cell, molecular and developmental biology
- No. of pages:
- © Academic Press 2017
- 28th August 2017
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Dr. Feinstein earned his undergraduate degree in Biochemistry at the University of California, Berkeley and his Ph.D. from the Department of Biochemistry and Biophysics at the University of California School of Medicine, San Francisco. Subsequently, he was a postdoctoral fellow in the Department of Neurobiology at the Stanford University School of Medicine until 1986, at which time he joined the faculty at UCSB. He has served as a grant reviewer for the National Institutes of Health, the National Science Foundation and the Cancer Research Coordinating Committee of California. In 2011, he was appointed a National Academies Education Fellow in the Life Sciences by the National Academies of Science and previously has been awarded a UCSB Distinguished Teaching Award. Dr. Feinstein is presently Co-Director of the Neuroscience Research Institute.
Co-Director, Neuroscience Research Institute, University of California, Santa Barbara
Dr. Lapointe has earned her undergraduate degree in Biology & Psychology at the University of Minnesota-Morris, and her Ph.D. in Neuroscience from Northwestern University. Subsequently, she was a postdoctoral fellow in University of California, Santa Barbara until 2013, and has continued to serve UCSB as an associate specialist. She is now an assistant project scientist at UCSB, and has participated in a number of research projects and publications, which her research is mainly focused on Tau proteins.
Assistant Project Scientist, University of California, Santa Barbara
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