- Print ISBN 9780123851185
- Electronic ISBN 9780123851192
Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research.
This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology.
With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research.
Section 1 – Strategies in systems biology. top down, middle-out and bottom-up strategies – Hans V. Westerhoff
Hans V. Westerhoff; OK
Section 2 – Machines for systems biology – Roy Goodacre
4 chapters explaining the workings of four of the most important measurement techniques for systems biology:
Section 3 – Nucleic acids and systems biology – James Adaye
4 Chapters on DNA sequencing, Arrays studies, PCR, deep sequencing
Section 4 - Protein production and quantification for Systems Biology –Naglis Malys and Kathleen Carroll
Chapter 1: Quantification of proteins and their modifications using QconCAT technology
Kathleen Carroll, Francesco Lanucara and Claire E. Eyers
Chapter 2: Mass spectrometric based quantitative proteomics using SILAC
Francesco Lanucara and Claire E. Eyers
Chapter 3: Nucleic Acid Programmable Protein Array (NAPPA) - a just-in-time multiplexed protein expression and purification platform
Chapter 4: Economical high throughput protein production and purification in prokaryotic and eukaryotic systems
Ron Seidel and James Love
Chapter 5: Systems biology of recombinant protein production using Bacillus megaterium
Rebekka Biedendieck and Dieter Jahn
Chapter 6: Protein production in S. cerevisiae for systems biology studies
Section 5 – Enzymatic Assays in Systems Biology Research Farid Khan, Hanan Messiha and Malgorzata Adamczyk
Chapter 1: Enzymatic Assays in Systems Biology Research: Strategies and
Chapter 2: Real-time kinetic assay technologies for characterising
enzymes in metabolic pathways.
- No. of pages:
- © 2011
- 26th September 2011
- Academic Press
- Print ISBN:
- Electronic ISBN: