Description

Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research.

Key Features

  • This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology
  • With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Contributors

Preface

Volume in series

Chapter one: Systems Biology Left and Right

1 A Fundamental Definition of Systems Biology

2 The Importance of the Integration of In Vivo and In Vitro Analyses

3 Alternative Definitions of Systems Biology

4 Different Types of Systems Biology

Chapter two: Mass Spectrometry in Systems Biology

1 Introduction

2 What is Mass Spectrometry?

3 Mass Spectrometer Configurations

4 The Benefits of Mass Spectrometry

5 Applications of Mass Spectrometry in Systems Biology

Chapter three: High-Throughput Quantification of Posttranslational Modifications In Situ by CA-FLIM

1 Introduction

2 Methods

3 Results and Discussion

4 Conclusion: Why Use CA-FLIM?

Appendix 1 Frequency Domain Fluorescence Lifetime Imaging Microscopy

Appendix 2 Global Analysis of FRET–FLIM Data

Appendix 3 Materials

Chapter four: Absorption Spectroscopy

1 Introduction

2 Theory of Absorption Spectroscopy

3 Hardware

4 Applications of UV–Visible Spectrometry

5 Perspective

Chapter five: A Strand-Specific Library Preparation Protocol for RNA Sequencing

1 Introduction

2 ssRNA-Seq Protocol

Chapter Six: Quantitative Real-Time PCR-Based Analysis of Gene Expression

1 Introduction

2 Primer Design

3 Primer Validation Experiments

4 Cell Culture-Based Isolation and Quantification of RNA

5 Reverse Transcription

Chapter seven: Quantification of Proteins and Their Modifications Using QconCAT Technology

1 Introduction

2 Expression and Purification of QconCAT Proteins

3 Preparation of Samples for Analysis

4 Mass Spectrometric Analysis

5 Quantification of Posttranslational Modifications

6 Replicate Analysis—Biological Versus Techni

Details

No. of pages:
668
Language:
English
Copyright:
© 2011
Published:
Imprint:
Academic Press
eBook ISBN:
9780123851192
Print ISBN:
9780123851185

About the serial-volume-editors

About the series-volume-editor