Description

Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research.

Key Features

This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology.

With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research.

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Section 1 – Strategies in systems biology. top down, middle-out and bottom-up strategies – Hans V. Westerhoff

Hans V. Westerhoff; OK

Section 2 – Machines for systems biology – Roy Goodacre

4 chapters explaining the workings of four of the most important measurement techniques for systems biology:

Mass spectrometry

Microscopy

Spectroscopy

Section 3 – Nucleic acids and systems biology – James Adaye

4 Chapters on DNA sequencing, Arrays studies, PCR, deep sequencing

Section 4 - Protein production and quantification for Systems Biology –Naglis Malys and Kathleen Carroll

Chapter 1: Quantification of proteins and their modifications using QconCAT technology

Kathleen Carroll, Francesco Lanucara and Claire E. Eyers

 

Chapter 2: Mass spectrometric based quantitative proteomics using SILAC

Francesco Lanucara and Claire E. Eyers

Chapter 3: Nucleic Acid Programmable Protein Array (NAPPA) - a just-in-time multiplexed protein expression and purification platform

Joshua LaBaer

Chapter 4: Economical high throughput protein production and purification in prokaryotic and eukaryotic systems

Ron Seidel and James Love

Chapter 5: Systems biology of recombinant protein production using Bacillus megaterium

Rebekka Biedendieck and Dieter Jahn

Chapter 6: Protein production in S. cerevisiae for systems biology studies

Naglis Malys

Section 5 – Enzymatic Assays in Systems Biology Research Farid Khan, Hanan Messiha and Malgorzata Adamczyk

Chapter 1: Enzymatic Assays in Systems Biology Research: Strategies and

challenges.

Farid Khan

Chapter 2: Real-time kinetic assay technologies for characterising

enzymes in metabolic pathways.

<

Details

No. of pages:
668
Language:
English
Copyright:
© 2011
Published:
Imprint:
Academic Press
Print ISBN:
9780123851185
Electronic ISBN:
9780123851192