Methods in Mammalian Reproduction

Methods in Mammalian Reproduction

1st Edition - January 1, 1978

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  • Editor: Joseph C. Jr. Daniel
  • eBook ISBN: 9780323145206

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Methods in Mammalian Reproduction presents some of the techniques for manipulating, analyzing, observing, testing, and generally experimenting with mammalian mothers and their gametes and embryos. Mammalian reproduction involves an intimate relationship between mother and embryo. The first 18 chapters are arranged in an order that follows a developmental sequence from oocyte to fetal organs and the remaining seven chapters deal with the maternal side of the relationship. With strong focus on laboratory rodents and lagomorphs, the book starts with an introduction to in vitro oocyte maturation and experimental production of mammalian parthenogenetic. It goes on to describe the microtechniques in pre-implantation of embryos, production of chimeras, techniques for early embryonic tissue separation, mammalian embryo preservation by freezing, and in vitro development of whole mouse embryos beyond the implantation stage. Chapters 11-15 discuss the in vitro implantation of mouse blastocysts, advances in rabbit embryo and in large mammal embryo cultures, embryo transfer in large domestic mammals, and manipulation of marsupial embryos and pouch young. The following chapters cover reproduction experiments using marsupials, domestic farm species, and primates including humans. Finally, the concluding chapters tackle the use of amniocentesis in prenatal diagnosis, collection and analysis of female genital tract secretions, analysis of antifertility action of intrauterine devices, and surgical induction of endometriosis. This book will be helpful to students, teachers, researchers, and clinical researchers who demand for more and better procedures for analysis of mammalian reproduction.

Table of Contents

  • List of Contributors


    1 In Vitro Oocyte Maturation

    I. General Introduction

    II. Methods Employed in the Culture of Oocytes

    III. Special Applications

    IV. Summary


    2 The Experimental Production of Mammalian Parthenogenetic Embryos

    I. Introduction

    II. Culture Methods Relevant to in Vitro Parthenogenetic Activation

    III. Isolation of Eggs from Superovulated and Spontaneously Ovulating Females

    IV. Reaction of Eggs to Parthenogenetic Stimulation

    V. Effect of Postovulatory Aging of the Oocyte on the Activation Frequency and Types of Parthenogenones Induced

    VI. Basic Techniques

    VII. Conclusions


    3 Manipulation of Ploidy in the Mouse

    I. Haploidy

    II. Triploidy

    III. Tetraploidy


    4 Methods for the High-Resolution Analysis of Protein Synthesis: Applications to Studies of Early Mammalian Development

    I. General Introduction

    II. Electrophoresis in SDS-Polyacrylamide Gradient Slab Gels

    III. Electrophoresis in Two-Dimensional Polyacrylamide Gels

    IV. Special Applications

    V. Problems of Resolution and Interpretation

    VI. Note Added in Proof


    5 Microtechniques with Preimplantation Embryos

    I. General Considerations

    II. Basis of Technique

    III. Preparation of Embryos for Analysis

    IV. Specific Assays of Metabolites in Embryos


    6 Production of Chimeras by Injecting Cells or Tissue into the Blastocyst

    I. Introduction

    II. Outline of the Approach

    III. Micromanipulation System

    IV. Equipment for Making Glass Micro-Instruments

    V. Preparation of the Micro-Instruments

    VI. Setting Up the Micro-Instruments on the Manipulator Assembly

    VII. Blastocyst Injection


    7 Techniques for Separating Early Embryonic Tissues

    I. Introduction

    II. The Embryos

    III. Tissue Separation

    IV. Other Possible Procedures


    8 Methods for the Preservation of Mammalian Embryos by Freezing

    I. Introduction

    II. Embryologic Methods

    III. Sample Preparation

    IV. Freezing and Thawing Procedures

    V. Sample Recovery

    VI. Conclusions


    9 Immunologic Inhibition of Development

    I. Introduction

    II. Antiserum Production and Collection

    III. Determination of Antiserum Activity and Specificity

    IV. Evaluation of Antibody Activity

    V. Summary


    10 In Vitro Development of Whole Mouse Embryos beyond the Implantation Stage

    I. Introduction

    II. Collagen

    III. Sera

    IV. Culture Media

    V. Obtaining Blastocysts from Mice

    VI. Preparation of Blastocysts

    VII. The Water-Jacketed, Automatic CO2 Control Incubators

    VIII. Mouse Development in Vitro

    IX. Proliferation of Trophoblast


    11 Implantation of Mouse Blastocysts in Vitro

    I. Introduction

    II. Preparation and Handling of Blastocysts

    III. Preparation of Uterine Monolayers

    IV. In Vitro Blastocyst Implantation Studies

    V. Prevention of Blastocyst Implantation in Vitro


    12 Advances in Rabbit Embryo Culture

    I. Introduction

    II. Preparation of Embryo Donors

    III. Embryo Collection

    IV. Culture Methods


    13 Advances in Large Mammal Embryo Culture

    I. Introduction

    II. Culture of Ovine Embryos

    III. Culture of Bovine Embryos


    14 Embryo Transfer in Large Domestic Mammals

    I. Introduction

    II. Facilities and Equipment

    III. Procedures

    IV. Future Directions


    15 Manipulation of Marsupial Embryos and Pouch Young

    I. Introduction

    II. Animal Husbandry to Obtain Timed Stages of Gestation

    III. Surgery

    IV. Manipulation of Early Embryos

    V. Collection of Fetal and Uterine Fluids and Tissues

    VI. Postnatal Development

    VII. Conclusions


    16 Experimentation Involving Primate Embryos

    I. Introduction

    II. Recovery of Ova and Embryos from the Female Reproductive Tract

    III. Gamete Recovery, in Vitro Fertilization, Embryo Culture, and Embryo Transfer

    IV. In Vitro Testing of Potentially Teratogenic Agents

    V. Conclusions


    17 Transplanting and Explanting Organ Primordia

    I. Transplanting Embryonic Tissues and Organs

    II. The Separation and Recombination of Embryonic Tissues

    III. A Few Comments on the in Vitro Culture of Organ Primordia


    18 The Regeneration of Mammalian Limbs and Limb Tissues

    I. Introduction

    II. Extremities

    III. Skeletal Muscle

    IV. Bone

    V. Peripheral Nerve

    VI. Concluding Remarks


    19 Pheromones, Estrus, Ovulation, and Mating

    I. Introduction

    II. Patterns of Reproduction in Laboratory Mammals

    III. The Role of Pheromones

    IV. The Exploitation of Pheromonal Effects

    V. Mating and Ovulation Soon after Pairing

    VI. Other Useful Techniques

    VII. Male Performance

    VIII. Summary


    20 Experimentally Delayed Implantation

    I. Introduction

    II. Implantation Delay as an Experimental Model

    III. Achievement and Maintenance of Implantation Delay

    IV. Reversal of Implantation Delay and Induction of Blastocyst Activation

    V. Implantation Delay in Vitro


    21 Laparoscopic Research Techniques in Mammalian Embryology

    I. Historical Aspects of Laparoscopy

    II. General Comments

    III. Laboratory Rodents, Rabbits, Birds, and Exotic Animals

    IV. Dogs and Cats

    V. Nonhuman Primates

    VI. Domestic Animals

    VII. Summary


    22 The Use of Amniocentesis in Prenatal Diagnosis

    I. Introduction

    II. Amniocentesis

    III. Ultrasound

    IV. Cell Culture Techniques

    V. Chromosome Analyses with Banding Techniques

    VI. Reported Experience with Human Prenatal Diagnosis

    VII. Problems, Ethics, and Future of Prenatal Diagnosis


    23 Collection and Analysis of Female Genital Tract Secretions

    I. Introduction

    II. Methods for Collection of Female Genital Tract Secretions

    III. Methods for Analysis of Female Genital Tract Secretions


    24 Experimental Approaches for Elucidating the Antifertility Action of Intrauterine Devices in Monkeys and Rodents

    I. Introduction

    II. Insertion of IUD’s

    III. Procedure for Embryo Recovery from Primate Uteri

    IV. Embryo Recovery in Mice

    V. Analysis of the Cell Content of Uterine Flushes from Monkeys and Rodents

    VI. Future Research


    25 Surgical Induction of Endometriosis

    I. Introduction

    II. Animals

    III. Surgery

    IV. Graft Identity



Product details

  • No. of pages: 584
  • Language: English
  • Copyright: © Academic Press 1978
  • Published: January 1, 1978
  • Imprint: Academic Press
  • eBook ISBN: 9780323145206

About the Editor

Joseph C. Jr. Daniel

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