Methods in Mammalian Reproduction - 1st Edition - ISBN: 9780122018503, 9780323145206

Methods in Mammalian Reproduction

1st Edition

Editors: Joseph C. Jr. Daniel
eBook ISBN: 9780323145206
Imprint: Academic Press
Published Date: 1st January 1978
Page Count: 584
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Methods in Mammalian Reproduction presents some of the techniques for manipulating, analyzing, observing, testing, and generally experimenting with mammalian mothers and their gametes and embryos. Mammalian reproduction involves an intimate relationship between mother and embryo. The first 18 chapters are arranged in an order that follows a developmental sequence from oocyte to fetal organs and the remaining seven chapters deal with the maternal side of the relationship.
With strong focus on laboratory rodents and lagomorphs, the book starts with an introduction to in vitro oocyte maturation and experimental production of mammalian parthenogenetic. It goes on to describe the microtechniques in pre-implantation of embryos, production of chimeras, techniques for early embryonic tissue separation, mammalian embryo preservation by freezing, and in vitro development of whole mouse embryos beyond the implantation stage. Chapters 11-15 discuss the in vitro implantation of mouse blastocysts, advances in rabbit embryo and in large mammal embryo cultures, embryo transfer in large domestic mammals, and manipulation of marsupial embryos and pouch young. The following chapters cover reproduction experiments using marsupials, domestic farm species, and primates including humans. Finally, the concluding chapters tackle the use of amniocentesis in prenatal diagnosis, collection and analysis of female genital tract secretions, analysis of antifertility action of intrauterine devices, and surgical induction of endometriosis. This book will be helpful to students, teachers, researchers, and clinical researchers who demand for more and better procedures for analysis of mammalian reproduction.

Table of Contents

List of Contributors


1 In Vitro Oocyte Maturation

I. General Introduction

II. Methods Employed in the Culture of Oocytes

III. Special Applications

IV. Summary


2 The Experimental Production of Mammalian Parthenogenetic Embryos

I. Introduction

II. Culture Methods Relevant to in Vitro Parthenogenetic Activation

III. Isolation of Eggs from Superovulated and Spontaneously Ovulating Females

IV. Reaction of Eggs to Parthenogenetic Stimulation

V. Effect of Postovulatory Aging of the Oocyte on the Activation Frequency and Types of Parthenogenones Induced

VI. Basic Techniques

VII. Conclusions


3 Manipulation of Ploidy in the Mouse

I. Haploidy

II. Triploidy

III. Tetraploidy


4 Methods for the High-Resolution Analysis of Protein Synthesis: Applications to Studies of Early Mammalian Development

I. General Introduction

II. Electrophoresis in SDS-Polyacrylamide Gradient Slab Gels

III. Electrophoresis in Two-Dimensional Polyacrylamide Gels

IV. Special Applications

V. Problems of Resolution and Interpretation

VI. Note Added in Proof


5 Microtechniques with Preimplantation Embryos

I. General Considerations

II. Basis of Technique

III. Preparation of Embryos for Analysis

IV. Specific Assays of Metabolites in Embryos


6 Production of Chimeras by Injecting Cells or Tissue into the Blastocyst

I. Introduction

II. Outline of the Approach

III. Micromanipulation System

IV. Equipment for Making Glass Micro-Instruments

V. Preparation of the Micro-Instruments

VI. Setting Up the Micro-Instruments on the Manipulator Assembly

VII. Blastocyst Injection


7 Techniques for Separating Early Embryonic Tissues

I. Introduction

II. The Embryos

III. Tissue Separation

IV. Other Possible Procedures


8 Methods for the Preservation of Mammalian Embryos by Freezing

I. Introduction

II. Embryologic Methods

III. Sample Preparation

IV. Freezing and Thawing Procedures

V. Sample Recovery

VI. Conclusions


9 Immunologic Inhibition of Development

I. Introduction

II. Antiserum Production and Collection

III. Determination of Antiserum Activity and Specificity

IV. Evaluation of Antibody Activity

V. Summary


10 In Vitro Development of Whole Mouse Embryos beyond the Implantation Stage

I. Introduction

II. Collagen

III. Sera

IV. Culture Media

V. Obtaining Blastocysts from Mice

VI. Preparation of Blastocysts

VII. The Water-Jacketed, Automatic CO2 Control Incubators

VIII. Mouse Development in Vitro

IX. Proliferation of Trophoblast


11 Implantation of Mouse Blastocysts in Vitro

I. Introduction

II. Preparation and Handling of Blastocysts

III. Preparation of Uterine Monolayers

IV. In Vitro Blastocyst Implantation Studies

V. Prevention of Blastocyst Implantation in Vitro


12 Advances in Rabbit Embryo Culture

I. Introduction

II. Preparation of Embryo Donors

III. Embryo Collection

IV. Culture Methods


13 Advances in Large Mammal Embryo Culture

I. Introduction

II. Culture of Ovine Embryos

III. Culture of Bovine Embryos


14 Embryo Transfer in Large Domestic Mammals

I. Introduction

II. Facilities and Equipment

III. Procedures

IV. Future Directions


15 Manipulation of Marsupial Embryos and Pouch Young

I. Introduction

II. Animal Husbandry to Obtain Timed Stages of Gestation

III. Surgery

IV. Manipulation of Early Embryos

V. Collection of Fetal and Uterine Fluids and Tissues

VI. Postnatal Development

VII. Conclusions


16 Experimentation Involving Primate Embryos

I. Introduction

II. Recovery of Ova and Embryos from the Female Reproductive Tract

III. Gamete Recovery, in Vitro Fertilization, Embryo Culture, and Embryo Transfer

IV. In Vitro Testing of Potentially Teratogenic Agents

V. Conclusions


17 Transplanting and Explanting Organ Primordia

I. Transplanting Embryonic Tissues and Organs

II. The Separation and Recombination of Embryonic Tissues

III. A Few Comments on the in Vitro Culture of Organ Primordia


18 The Regeneration of Mammalian Limbs and Limb Tissues

I. Introduction

II. Extremities

III. Skeletal Muscle

IV. Bone

V. Peripheral Nerve

VI. Concluding Remarks


19 Pheromones, Estrus, Ovulation, and Mating

I. Introduction

II. Patterns of Reproduction in Laboratory Mammals

III. The Role of Pheromones

IV. The Exploitation of Pheromonal Effects

V. Mating and Ovulation Soon after Pairing

VI. Other Useful Techniques

VII. Male Performance

VIII. Summary


20 Experimentally Delayed Implantation

I. Introduction

II. Implantation Delay as an Experimental Model

III. Achievement and Maintenance of Implantation Delay

IV. Reversal of Implantation Delay and Induction of Blastocyst Activation

V. Implantation Delay in Vitro


21 Laparoscopic Research Techniques in Mammalian Embryology

I. Historical Aspects of Laparoscopy

II. General Comments

III. Laboratory Rodents, Rabbits, Birds, and Exotic Animals

IV. Dogs and Cats

V. Nonhuman Primates

VI. Domestic Animals

VII. Summary


22 The Use of Amniocentesis in Prenatal Diagnosis

I. Introduction

II. Amniocentesis

III. Ultrasound

IV. Cell Culture Techniques

V. Chromosome Analyses with Banding Techniques

VI. Reported Experience with Human Prenatal Diagnosis

VII. Problems, Ethics, and Future of Prenatal Diagnosis


23 Collection and Analysis of Female Genital Tract Secretions

I. Introduction

II. Methods for Collection of Female Genital Tract Secretions

III. Methods for Analysis of Female Genital Tract Secretions


24 Experimental Approaches for Elucidating the Antifertility Action of Intrauterine Devices in Monkeys and Rodents

I. Introduction

II. Insertion of IUD’s

III. Procedure for Embryo Recovery from Primate Uteri

IV. Embryo Recovery in Mice

V. Analysis of the Cell Content of Uterine Flushes from Monkeys and Rodents

VI. Future Research


25 Surgical Induction of Endometriosis

I. Introduction

II. Animals

III. Surgery

IV. Graft Identity




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© Academic Press 1978
Academic Press
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About the Editor

Joseph C. Jr. Daniel

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