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1. Single glomerular proteomics – a novel tool for translational glomerular cell biology
2. Kidney harvesting and metabolite extraction for metabolomics studies in rodents
Maria Irazabal Mira
3. Optical tissue clearing and immunolabeling in kidney research
4. Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease
5. Intravital Visualization of the Primary Cilium, Tubule Flow, and Innate Immune Cells in the Kidney Utilizing an Abdominal Window Imaging Approach
6. Novel fluorescence techniques to quantitate renal cell biology
7. Manipulation of Renal Gene Expression Using Oligonucleotides
8. Methods for renal lineage tracing: in vivo and beyond
9. In Vivo Magnetic Resonance Imaging Techniques for Structural and Functional Characterization of Murine Model Kidneys
10. In vivo analysis of renal epithelial cells in zebrafish
11. Visualizing gene expression during zebrafish pronephros development and regeneration
12. Drosophila melanogaster and its nephrocytes – a versatile model for glomerular research
Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
- Written by experts in their field who have perfected stated methods
- Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form
- Includes cutting-edge, recently developed methods
Anyone conducting renal research from novice to veteran and from technicians to graduate students to postdoctoral fellows and principal investigators
- No. of pages:
- © Academic Press 2019
- 1st September 2019
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Dr. Weimbs received his doctoral degree from the Department of Biochemistry of the University of Cologne, Germany, in 1993. He conducted postdoctoral research with Keith Mostov at the Department of Anatomy, University of California at San Francisco until 1999 where he investigated the role of SNARE proteins in membrane trafficking and epithelial cell polarity. In 1999, he joined the Department of Cell Biology in the Lerner Research Institute of the Cleveland Clinic as an Assistant Professor where he established his own research laboratory. Besides continuing his work on SNAREs and epithelial cell polarity his laboratory began to investigate molecular mechanisms underlying polycystic kidney disease (PKD). In 2005, Dr. Weimbs moved his lab to the University of California in Santa Barbara where he is currently a Professor in the Department of Molecular, Cellular, and Developmental Biology. Research on PKD in Dr. Weimbs’ laboratory has contributed to our understanding of the molecular pathogenesis and the function of polycystin-1, the protein affected in this disease. These contributions include the roles of mTOR and STAT signaling in PKD. Recent work has focused on developing new kidney-targeted therapeutics, the role of metabolic changes and tubular crystal deposition in PKD disease progression, and the use of dietary interventions for PKD therapy.
University of California, Santa Barbara, USA