Methods in Kidney Cell Biology Part B

Methods in Kidney Cell Biology Part B

1st Edition - September 1, 2019
  • Editor: Thomas Weimbs
  • Hardcover ISBN: 9780128203354
  • eBook ISBN: 9780128203361

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Description

Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.

Key Features

  • Written by experts in their field who have perfected stated methods
  • Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form
  • Includes cutting-edge, recently developed methods

Readership

Anyone conducting renal research from novice to veteran and from technicians to graduate students to postdoctoral fellows and principal investigators

Table of Contents

  • 1. Single glomerular proteomics – a novel tool for translational glomerular cell biology
    Markus Rinschen 
    2. Kidney harvesting and metabolite extraction for metabolomics studies in rodents
    Maria Irazabal Mira 
    3. Optical tissue clearing and immunolabeling in kidney research
    David Ellison
    4. Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease
    Roman Müller 
    5. Intravital Visualization of the Primary Cilium, Tubule Flow, and Innate Immune Cells in the Kidney Utilizing an Abdominal Window Imaging Approach
    Bradley Yoder
    6. Novel fluorescence techniques to quantitate renal cell biology
    Janos Peti-Peterdi 
    7. Manipulation of Renal Gene Expression Using Oligonucleotides
    Vishal Patel
    8. Methods for renal lineage tracing: in vivo and beyond
    Thomas Carroll
    9. In Vivo Magnetic Resonance Imaging Techniques for Structural and Functional Characterization of Murine Model Kidneys
    Tim Kline
    10. In vivo analysis of renal epithelial cells in zebrafish
    Zhaoxia Sun
    11. Visualizing gene expression during zebrafish pronephros development and regeneration
    Rebecca Wingert
    12. Drosophila melanogaster and its nephrocytes – a versatile model for glomerular research
    Paul Brinkkotter

Product details

  • No. of pages: 254
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: September 1, 2019
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128203354
  • eBook ISBN: 9780128203361

About the Serial Volume Editor

Thomas Weimbs

Thomas Weimbs
Dr. Weimbs received his doctoral degree from the Department of Biochemistry of the University of Cologne, Germany, in 1993. He conducted postdoctoral research with Keith Mostov at the Department of Anatomy, University of California at San Francisco until 1999 where he investigated the role of SNARE proteins in membrane trafficking and epithelial cell polarity. In 1999, he joined the Department of Cell Biology in the Lerner Research Institute of the Cleveland Clinic as an Assistant Professor where he established his own research laboratory. Besides continuing his work on SNAREs and epithelial cell polarity his laboratory began to investigate molecular mechanisms underlying polycystic kidney disease (PKD). In 2005, Dr. Weimbs moved his lab to the University of California in Santa Barbara where he is currently a Professor in the Department of Molecular, Cellular, and Developmental Biology. Research on PKD in Dr. Weimbs’ laboratory has contributed to our understanding of the molecular pathogenesis and the function of polycystin-1, the protein affected in this disease. These contributions include the roles of mTOR and STAT signaling in PKD. Recent work has focused on developing new kidney-targeted therapeutics, the role of metabolic changes and tubular crystal deposition in PKD disease progression, and the use of dietary interventions for PKD therapy.

Affiliations and Expertise

University of California, Santa Barbara, USA