Methods in Cell Physiology

Methods in Cell Physiology

Volume I

1st Edition - January 1, 1964

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  • Editor: David M. Prescott
  • eBook ISBN: 9781483264035

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Description

Methods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which have not been published in full form elsewhere in the literature. The presentations in this volume are comprehensive to the extent that they may serve not only as a practical introduction to experimental procedures but they also provide some evaluation of the limitations, potentialities, and current applications of the methods. Only those theoretical considerations needed for proper use of the method are included. The book may have particular usefulness for those working with intact cells, and the first chapters deal with culturing and experimental manipulation of a variety of cell types. There are numerous descriptions of techniques designed for working with single cells and of procedures for studying cellular activities in relation to the cell life cycle. The chapters on autoradiography include detailed treatment of qualitative and quantitative aspects of the method as well as simple procedures for using liquid and dry emulsions.

Table of Contents


  • List of Contributors

    Preface

    1. Survey of Cytochemistry

    I. Survey

    II. Summary and Conclusions

    References

    2. Methods of Culture for Plasmodial Myxomycetes

    I. Introduction

    II. Culture of Impure Myxomycètes

    III. Isolation and Pure Culture

    IV. Axenic Culture of Physarum polycephalum on Soluble Media

    V. Sporulation (Fruiting) of Physarum polycephalum in Pure Culture

    VI. Sclerotia and Spherule Formation

    VII. Changes Observed after Prolonged Growth in Pure Culture

    References

    3. Mitotic Synchrony in the Plasmodia of Physarum Polycephalum and Mitotic Synchronization by Coalescence of Microplasmodia

    I. Introduction

    II. Life History of Physarum Polycephalum

    III. The Experimental Material

    IV. Discussion

    V. Summary

    References

    4. Introduction of Synchronous Encystment (Differentiation) in Acanthamoeba sp.

    I. Introduction

    II. The Encystment Process: A Description

    III. Induction of Synchronous Encystment: The Method

    IV. Optimal Conditions for Synchronous Encystment

    V. Evaluation of the Method

    References

    5. Experimental Procedures and Cultural Methods for Euplotes Eurystomus and Amoeba Proteus

    I. Introduction

    II. Euplotes Eurystomus

    III. Amoeba Proteus

    References

    6. Nuclear Transplantation in Ameba

    I. Introduction

    II. Equipment

    III. Operating Procedure

    IV. Conclusion

    References

    7. Experimental Techniques with Ciliates

    I. Introduction

    II. Experimental Possibilities with Stentor

    III. Techniques with Paramecium

    References

    8. Methods for Using Tetrahymena in Studies of the Normal Cell Cycle

    I. Introduction

    II. Special Equipment

    III. Manipulation of Cells

    References

    9. Continuous Synchronous Cultures of Protozoa

    I. Introduction

    II. Light-Induced Synchrony in Euglena Gracilis

    III. Temperature-Induced Synchrony in Astasia Longa

    IV. Concluding Remarks

    References

    10. Handling and Culturing of Chlorella

    I. Introduction

    II. The Organisms and Their Growth

    III. The Culture of Chlorella

    Appendix

    References

    11. Culturing and Experimental Manipulation of Acetabularia

    I. Introduction to the Organism

    II. Culture Techniques

    III. Microsurgery

    IV. Biochemical Analysis

    V. Potentialities and Limitations

    References

    12. Handling of Root Tips

    I. Introduction

    II. Germination of Seeds and Growing of Roots

    III. Fixation of Root Tips

    IV. Staining of Cells

    V. Making Slides Permanent

    VI. Autoradiographic Methods

    VII. Isolation of Nuclei

    VIII. Special Uses

    IX.Conclusion

    References

    13. Grasshopper Neuroblast Techniques

    I. Introduction

    II. Selection of Species

    III. Collecting

    IV. Rearing

    V. Eggs

    VI. Development

    VII. Culture Techniques

    VIII. Separated Cell Techniques

    IX. Fixed Cell Techniques

    X. Radiation Techniques

    XI. Chemical Techniques

    XII. Gas Techniques

    XIII. Microdissection

    References

    14. Measurement of Material Uptake by Cells: Pinocytosis

    I. Introduction

    II. Demonstration of Pinocytosis

    III. Inducers of Pinocytosis

    IV. Quantitative Estimation of Pinocytic Uptake

    V. Significance of the Values Obtained and Errors Likely to Be Encountered in the Different Methods

    References

    15. Quantitative Autoradiography

    I. Introduction

    II. Theoretical Considerations

    III. Practical Considerations and Techniques

    IV. The Problem of "Intermediates"

    References

    16. High-Resolution Autoradiography

    I. Introduction

    II. Methods

    III. Discussion of the Methods

    IV. Resolution

    V. Discussion

    References

    17. Autoradiography with Liquid Emulsion

    I. Introduction

    II. Emulsions

    III. Procedure

    References

    18. Autoradiography of Water-Soluble Materials

    I. Introduction

    II. Procedures

    III. Experimental Example

    References

    19. Preparation of Mammalian Metaphase Chromosomes for Autoradiography

    I. Introduction

    II. Procedure

    III. Examples and Variation of Technique

    References

    20. Methods for Measuring the Length of the Mitotic Cycle and the Timing of DNA Synthesis for Mammalian Cells in Culture

    I. Introduction

    II. Measurements of Intermitotic Times by Time-Lapse

    III. Cinemicrography

    IV. Measurements of Growth Rates of Cultures

    V. Measurement of Parts of Interphase

    VI. Summary

    References

    21. Micrurgy of Tissue Culture Cells

    I. Introduction

    II. Equipment

    III. Handling of Cells

    IV. Conclusion

    References

    22. Microextraction and Microelectrophoresis for Determination and Analysis of Nucleic Acids in Isolated Cellular Units

    I. Introduction

    II. Methods

    III. Accuracy

    IV. Applications

    V. Concluding Remarks

    VI. Appendices

    References

    Author Index

    Subject Index

Product details

  • No. of pages: 480
  • Language: English
  • Copyright: © Academic Press 1964
  • Published: January 1, 1964
  • Imprint: Academic Press
  • eBook ISBN: 9781483264035

About the Editor

David M. Prescott

Affiliations and Expertise

BIOLOGY DIVISION, OAK RIDGE NATIONAL LABORATORY OAK RIDGE, TENNESSEE

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