mechanistic studies of DNA replication and genetic recombination - 1st Edition - ISBN: 9780120488506, 9780323141796

mechanistic studies of DNA replication and genetic recombination

1st Edition

Editors: Bruce Alberts
eBook ISBN: 9780323141796
Imprint: Academic Press
Published Date: 1st January 1980
Page Count: 1034
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Mechanistic Studies of DNA Replication and Genetic Recombination emerged from a symposium on DNA replication and genetic recombination held from March 16-21, 1980 in Keystone, Colorado. The event featured 30 plenary session talks, 13 workshop discussion groups, and the 210 poster sessions. The studies described in this book are paving the way for the elucidation of other basic genetic mechanisms, including ""new"" areas in molecular genetics such as those of eukaryotic gene expression and the transposition of mobile genetic elements. This book is divided into 10 parts: summaries of workshop discussion groups (Part I); studies on eukaryotic model systems for DNA replication (Part II); studies on bacterial replication origins (Part III); studies on replication origins of bacterial phages and plasmids (Part IV); studies on eukaryotic replication origins (Part V); studies on prokaryotic replication enzymology (Part VI); studies on eukaryotic replication enzymology (Part VII); studies on the fidelity of DNA replication (Part VIII); studies on DNA topoisomerases (Part IX); and studies of genetic recombination mechanisms (Part X).

Table of Contents




I. Summaries of Workshop Discussion Groups

1 Regulation of Plasmid Replication

2 Genetic Analysis of DNA Replication

3 Procaryotic Replication Systems

4 RNA Priming

5 Replicating Linear DNA Molecules

6 DNA Replication and Chromosome Structure

7 DNA Replication and Genetic Recombination

8 Eucaryotic DNA Polymerases and Other Eucaryotic Replication Proteins

9 Site-Specific Recombination Mechanisms

II. Eukaryotic Model Systems for DNA Replication

10 Binding of D2 Protein to Specific Papovavirus Sequences

11 The Interdependence of DNA Replication, DNA Sequence, and Chromatin Structure in Simian Virus 40 Chromosomes

12 The Unique Mechanism of Animal Mitochondrial DNA Replication

13 Adenovirus DNA Replication: Possible Roles of the Covalently Bound Terminal Protein

14 Adenovirus DNA in Vitro Replication and Recombination

15 Adeno-Associated Virus DNA Replication

16 In Vitro Synthesis of Parvoviral DNA

III. Bacterial Replication Origins

17 The Origin of Replication, oriC, of the Escherichia coli Chromosome: Genes Near oriC and Construction of oriC Deletion Mutations

18 Structure of the E. coli Replication Origin. oriC Sequences Required for Maintenance, Establishment, and Bidirectional Replication

19 The Effects of oriC-Containing Plasmids on Host Cell Growth

20 The Salmonella typhimurium Origin of DNA Replication

21 Isolation of a Membrane Protein Having an Affinity to the Replication Origin of E. coli

22 Properties of an Isolated Replicative Origin Complex from the Chromosome of Escherichia coli

23 DNA-Membrane Association and Initiation of DNA Replication of Bacillus subtilis

IV. Replication Origins of Bacterial Phages and Plasmids

24 Organization of Sequences at Lambdoid Bacteriophage Origins of Replication

25 Origin and Inceptor of DNA Replication in Bacteriophage Lambda

26 Protein p3 Covalently Linked to the 5' Termini of ϕ29 DNA and Its Possible Role as Primer in the Initiation of DNA Replication

27 Studies on the Origin of ϕX RF DNA Replication

28 Tertiary Structure Is Involved in the Initiation of DNA Synthesis by the dnaG Protein

29 Structure of the Replication Origins of Plasmids RK2 and R6K and the Regulation of Plasmid DNA Replication

30 Replication, Incompatibility, and Stability Functions of R Plasmid NR1

31 Regulation of Replication of the PI Plasmid Prophage

V. Studies on Eukaryotic Replication Origins

32 Complexity of Chromosomal Replication Origins in Saccharomyces cerevisiae

33 Isolation of Eukaryote DNA Replication Origins

34 Analysis of Cloned Human Ubiquitous Repeated DNA Sequences

35 Preferential Utilization of a Yeast Chromosomal Replication Origin as Template for Enzymatic DNA Synthesis

36 Replication Functions Associated with the Yeast Plasmid 2μ Circle

37 Replication of Small Chromosomal DNAs in Yeast

VI. Prokaryotic Replication Enzymology

38 Replication of Bacteriophage T7 DNA

39 Mapping of Initiation Sites of DNA Replication on Prokaryotic Genomes

40 Studies of Replication Mechanisms with the T4 Bacteriophage in Vitro System

41 RNA Priming and DNA Synthesis by Bacteriophage T4 Proteins

42 Molecular Aspects of the Interactions of T4-Coded Gene 32-Protein and DNA Polymerase (Gene 43-Protein) with Nucleic Acids

43 The Control of T4 Gene 32 Helix-Destabilizing Protein Activity in a DNA Replication Complex

44 A Chimeric Plasmid Containing Gene 32 of Bacteriophage T4D

45 Genetic Analysis of DNA Replication in Bacteriophage T4

46 Enzyme Studies of μX174 DNA Replication

47 Multiple Forms of DNA Polymerase III of Escherichia coli

48 Replication of Phage fd DNA with Purified Proteins

49 The Primary Structure of DNA Polymerase I of E. coli

50 DNA Polymerase I-Dependent Replication

51 Isolation of DNA-Dependent ATPase I Mutants of E. coli

52 Studies with the Escherichia coli dnaJ and dnaK Genes

53 Interaction of Bacteriophage Lambda Replication Proteins with Host Cell Components

VII. Eukaryotic Replication Enzymology

54 Rat Liver DNA Binding Protein: Properties and Physiological Variations

55 Characterization of Mammalian DNase V, a Double-Strand Bidirectional Exonuclease which Interacts with DNA Polymerase-β of the Novikoff Hepatoma

56 Structure of DNA Polymerase-α

57 KB Cell DNA Polymerase-α: Mechanism of Primer-Template Recognition by the Core Catalytic Unit

58 Diadenosine Tetraphosphate-A Ligand of DNA Polymerase-α and Trigger of DNA Replication

59 Activation of the Drosophila Embryo DNA Polymerase by Proteolytic Cleavage

VIII. Studies on the Fidelity of DNA Replication

60 On the Molecular Basis of Mutagenesis: Enzymological and Genetic Studies with the Bacteriophage T4 System

61 Probing the Mechanism of Transition and Transversion Mutagenesis Using the Phage T4 DNA Replication Apparatus in Vitro 707

62 Studies of the Effects of Chemical Mutagens on the Fidelity of DNA Replication

63 Fidelity of Copying Natural DNA Templates

64 Base Mispairing and Nearest-Neighbor Effects in Transition Mutations

IX. DNA Topoisomerases

65 DNA Topoisomerases

66 Mechanisms of DNA Topoisomerases

67 Topoisomerases and the Swivel Problem

68 DNA Strand Passing and the Function of Type II DNA Topoisomerases

69 Isolation of an E. coli DNA Topoisomerase I Mutant

X. Studies of Genetic Recombination Mechanisms

70 Genetic Recombination and the Fusion of DNA Molecules

71 rec A Protein of E. coli Promotes Homologous Pairing of DNA Molecules by a Novel Mechanism

72 Initiation of Genetic Recombination: Joining of Duplex DNA Molecules Promoted by rec A Protein

73 rec A-Dependent DNA Replication in E. coli: A New Mutation Affecting the Ability of recA to Regulate DNA Replication

74 A View of the recBC and rec F Pathways of E. coli Recombination

75 Electron Microscopy of E. coli recBC Enzyme Reaction Intermediates

76 Unwinding and Rewinding of DNA by Exonuclease V

77 What Accounts for the Orientation Dependence and Directionality of Chi?

78 Nucleotide Sequence of Chi Recombinational Hotspots

79 Genetic Recombination of Bacterial Plasmid DNAs in Vivo and in Vitro

80 Genetic Recombination of Bacteriophage T7 DNA in Vitro Synthesis

81 Catenation of the Products of Integrative Recombination: Comparison of Supertwisted and Nonsupertwisted Substrates

82 PI Site-Specific Recombination

83 Genetic Analysis of Transposition and Excision by Transposon Tn10

84 Tn 554: Site Specificity of Insertion and Characterization of Secondary Site Insertions



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© Academic Press 1980
Academic Press
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About the Editor

Bruce Alberts

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