mechanistic studies of DNA replication and genetic recombination

Contributors

Preface

Acknowledgments

I. Summaries of Workshop Discussion Groups


1 Regulation of Plasmid Replication


2 Genetic Analysis of DNA Replication


3 Procaryotic Replication Systems


4 RNA Priming


5 Replicating Linear DNA Molecules


6 DNA Replication and Chromosome Structure


7 DNA Replication and Genetic Recombination


8 Eucaryotic DNA Polymerases and Other Eucaryotic Replication Proteins


9 Site-Specific Recombination Mechanisms

II. Eukaryotic Model Systems for DNA Replication


10 Binding of D2 Protein to Specific Papovavirus Sequences


11 The Interdependence of DNA Replication, DNA Sequence, and Chromatin Structure in Simian Virus 40 Chromosomes


12 The Unique Mechanism of Animal Mitochondrial DNA Replication


13 Adenovirus DNA Replication: Possible Roles of the Covalently Bound Terminal Protein


14 Adenovirus DNA in Vitro Replication and Recombination


15 Adeno-Associated Virus DNA Replication


16 In Vitro Synthesis of Parvoviral DNA

III. Bacterial Replication Origins


17 The Origin of Replication, oriC, of the Escherichia coli Chromosome: Genes Near oriC and Construction of oriC Deletion Mutations


18 Structure of the E. coli Replication Origin. oriC Sequences Required for Maintenance, Establishment, and Bidirectional Replication


19 The Effects of oriC-Containing Plasmids on Host Cell Growth


20 The Salmonella typhimurium Origin of DNA Replication


21 Isolation of a Membrane Protein Having an Affinity to the Replication Origin of E. coli


22 Properties of an Isolated Replicative Origin Complex from the Chromosome of Escherichia coli


23 DNA-Membrane Association and Initiation of DNA Replication of Bacillus subtilis

IV. Replication Origins of Bacterial Phages and Plasmids


24 Organization of Sequences at Lambdoid Bacteriophage Origins of Replication


25 Origin and Inceptor of DNA Replication in Bacteriophage Lambda


26 Protein p3 Covalently Linked to the 5' Termini of ϕ29 DNA and Its Possible Role as Primer in the Initiation of DNA Replication


27 Studies on the Origin of ϕX RF DNA Replication


28 Tertiary Structure Is Involved in the Initiation of DNA Synthesis by the dnaG Protein


29 Structure of the Replication Origins of Plasmids RK2 and R6K and the Regulation of Plasmid DNA Replication


30 Replication, Incompatibility, and Stability Functions of R Plasmid NR1


31 Regulation of Replication of the PI Plasmid Prophage

V. Studies on Eukaryotic Replication Origins


32 Complexity of Chromosomal Replication Origins in Saccharomyces cerevisiae


33 Isolation of Eukaryote DNA Replication Origins


34 Analysis of Cloned Human Ubiquitous Repeated DNA Sequences


35 Preferential Utilization of a Yeast Chromosomal Replication Origin as Template for Enzymatic DNA Synthesis


36 Replication Functions Associated with the Yeast Plasmid 2μ Circle


37 Replication of Small Chromosomal DNAs in Yeast

VI. Prokaryotic Replication Enzymology


38 Replication of Bacteriophage T7 DNA


39 Mapping of Initiation Sites of DNA Replication on Prokaryotic Genomes


40 Studies of Replication Mechanisms with the T4 Bacteriophage in Vitro System


41 RNA Priming and DNA Synthesis by Bacteriophage T4 Proteins


42 Molecular Aspects of the Interactions of T4-Coded Gene 32-Protein and DNA Polymerase (Gene 43-Protein) with Nucleic Acids


43 The Control of T4 Gene 32 Helix-Destabilizing Protein Activity in a DNA Replication Complex


44 A Chimeric Plasmid Containing Gene 32 of Bacteriophage T4D


45 Genetic Analysis of DNA Replication in Bacteriophage T4


46 Enzyme Studies of μX174 DNA Replication


47 Multiple Forms of DNA Polymerase III of Escherichia coli


48 Replication of Phage fd DNA with Purified Proteins


49 The Primary Structure of DNA Polymerase I of E. coli


50 DNA Polymerase I-Dependent Replication


51 Isolation of DNA-Dependent ATPase I Mutants of E. coli


52 Studies with the Escherichia coli dnaJ and dnaK Genes


53 Interaction of Bacteriophage Lambda Replication Proteins with Host Cell Components

VII. Eukaryotic Replication Enzymology


54 Rat Liver DNA Binding Protein: Properties and Physiological Variations


55 Characterization of Mammalian DNase V, a Double-Strand Bidirectional Exonuclease which Interacts with DNA Polymerase-β of the Novikoff Hepatoma


56 Structure of DNA Polymerase-α


57 KB Cell DNA Polymerase-α: Mechanism of Primer-Template Recognition by the Core Catalytic Unit


58 Diadenosine Tetraphosphate-A Ligand of DNA Polymerase-α and Trigger of DNA Replication


59 Activation of the Drosophila Embryo DNA Polymerase by Proteolytic Cleavage

VIII. Studies on the Fidelity of DNA Replication


60 On the Molecular Basis of Mutagenesis: Enzymological and Genetic Studies with the Bacteriophage T4 System


61 Probing the Mechanism of Transition and Transversion Mutagenesis Using the Phage T4 DNA Replication Apparatus in Vitro 707


62 Studies of the Effects of Chemical Mutagens on the Fidelity of DNA Replication


63 Fidelity of Copying Natural DNA Templates


64 Base Mispairing and Nearest-Neighbor Effects in Transition Mutations

IX. DNA Topoisomerases


65 DNA Topoisomerases


66 Mechanisms of DNA Topoisomerases


67 Topoisomerases and the Swivel Problem


68 DNA Strand Passing and the Function of Type II DNA Topoisomerases


69 Isolation of an E. coli DNA Topoisomerase I Mutant

X. Studies of Genetic Recombination Mechanisms


70 Genetic Recombination and the Fusion of DNA Molecules


71 rec A Protein of E. coli Promotes Homologous Pairing of DNA Molecules by a Novel Mechanism


72 Initiation of Genetic Recombination: Joining of Duplex DNA Molecules Promoted by rec A Protein


73 rec A-Dependent DNA Replication in E. coli: A New Mutation Affecting the Ability of recA to Regulate DNA Replication


74 A View of the recBC and rec F Pathways of E. coli Recombination


75 Electron Microscopy of E. coli recBC Enzyme Reaction Intermediates


76 Unwinding and Rewinding of DNA by Exonuclease V


77 What Accounts for the Orientation Dependence and Directionality of Chi?


78 Nucleotide Sequence of Chi Recombinational Hotspots


79 Genetic Recombination of Bacterial Plasmid DNAs in Vivo and in Vitro


80 Genetic Recombination of Bacteriophage T7 DNA in Vitro Synthesis


81 Catenation of the Products of Integrative Recombination: Comparison of Supertwisted and Nonsupertwisted Substrates


82 PI Site-Specific Recombination


83 Genetic Analysis of Transposition and Excision by Transposon Tn10


84 Tn 554: Site Specificity of Insertion and Characterization of Secondary Site Insertions

Index