mechanistic studies of DNA replication and genetic recombination

mechanistic studies of DNA replication and genetic recombination

1st Edition - January 1, 1980

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  • Editor: Bruce Alberts
  • eBook ISBN: 9780323141796

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Mechanistic Studies of DNA Replication and Genetic Recombination emerged from a symposium on DNA replication and genetic recombination held from March 16-21, 1980 in Keystone, Colorado. The event featured 30 plenary session talks, 13 workshop discussion groups, and the 210 poster sessions. The studies described in this book are paving the way for the elucidation of other basic genetic mechanisms, including ""new"" areas in molecular genetics such as those of eukaryotic gene expression and the transposition of mobile genetic elements. This book is divided into 10 parts: summaries of workshop discussion groups (Part I); studies on eukaryotic model systems for DNA replication (Part II); studies on bacterial replication origins (Part III); studies on replication origins of bacterial phages and plasmids (Part IV); studies on eukaryotic replication origins (Part V); studies on prokaryotic replication enzymology (Part VI); studies on eukaryotic replication enzymology (Part VII); studies on the fidelity of DNA replication (Part VIII); studies on DNA topoisomerases (Part IX); and studies of genetic recombination mechanisms (Part X).

Table of Contents

  • Contributors



    I. Summaries of Workshop Discussion Groups

    1 Regulation of Plasmid Replication

    2 Genetic Analysis of DNA Replication

    3 Procaryotic Replication Systems

    4 RNA Priming

    5 Replicating Linear DNA Molecules

    6 DNA Replication and Chromosome Structure

    7 DNA Replication and Genetic Recombination

    8 Eucaryotic DNA Polymerases and Other Eucaryotic Replication Proteins

    9 Site-Specific Recombination Mechanisms

    II. Eukaryotic Model Systems for DNA Replication

    10 Binding of D2 Protein to Specific Papovavirus Sequences

    11 The Interdependence of DNA Replication, DNA Sequence, and Chromatin Structure in Simian Virus 40 Chromosomes

    12 The Unique Mechanism of Animal Mitochondrial DNA Replication

    13 Adenovirus DNA Replication: Possible Roles of the Covalently Bound Terminal Protein

    14 Adenovirus DNA in Vitro Replication and Recombination

    15 Adeno-Associated Virus DNA Replication

    16 In Vitro Synthesis of Parvoviral DNA

    III. Bacterial Replication Origins

    17 The Origin of Replication, oriC, of the Escherichia coli Chromosome: Genes Near oriC and Construction of oriC Deletion Mutations

    18 Structure of the E. coli Replication Origin. oriC Sequences Required for Maintenance, Establishment, and Bidirectional Replication

    19 The Effects of oriC-Containing Plasmids on Host Cell Growth

    20 The Salmonella typhimurium Origin of DNA Replication

    21 Isolation of a Membrane Protein Having an Affinity to the Replication Origin of E. coli

    22 Properties of an Isolated Replicative Origin Complex from the Chromosome of Escherichia coli

    23 DNA-Membrane Association and Initiation of DNA Replication of Bacillus subtilis

    IV. Replication Origins of Bacterial Phages and Plasmids

    24 Organization of Sequences at Lambdoid Bacteriophage Origins of Replication

    25 Origin and Inceptor of DNA Replication in Bacteriophage Lambda

    26 Protein p3 Covalently Linked to the 5' Termini of ϕ29 DNA and Its Possible Role as Primer in the Initiation of DNA Replication

    27 Studies on the Origin of ϕX RF DNA Replication

    28 Tertiary Structure Is Involved in the Initiation of DNA Synthesis by the dnaG Protein

    29 Structure of the Replication Origins of Plasmids RK2 and R6K and the Regulation of Plasmid DNA Replication

    30 Replication, Incompatibility, and Stability Functions of R Plasmid NR1

    31 Regulation of Replication of the PI Plasmid Prophage

    V. Studies on Eukaryotic Replication Origins

    32 Complexity of Chromosomal Replication Origins in Saccharomyces cerevisiae

    33 Isolation of Eukaryote DNA Replication Origins

    34 Analysis of Cloned Human Ubiquitous Repeated DNA Sequences

    35 Preferential Utilization of a Yeast Chromosomal Replication Origin as Template for Enzymatic DNA Synthesis

    36 Replication Functions Associated with the Yeast Plasmid 2μ Circle

    37 Replication of Small Chromosomal DNAs in Yeast

    VI. Prokaryotic Replication Enzymology

    38 Replication of Bacteriophage T7 DNA

    39 Mapping of Initiation Sites of DNA Replication on Prokaryotic Genomes

    40 Studies of Replication Mechanisms with the T4 Bacteriophage in Vitro System

    41 RNA Priming and DNA Synthesis by Bacteriophage T4 Proteins

    42 Molecular Aspects of the Interactions of T4-Coded Gene 32-Protein and DNA Polymerase (Gene 43-Protein) with Nucleic Acids

    43 The Control of T4 Gene 32 Helix-Destabilizing Protein Activity in a DNA Replication Complex

    44 A Chimeric Plasmid Containing Gene 32 of Bacteriophage T4D

    45 Genetic Analysis of DNA Replication in Bacteriophage T4

    46 Enzyme Studies of μX174 DNA Replication

    47 Multiple Forms of DNA Polymerase III of Escherichia coli

    48 Replication of Phage fd DNA with Purified Proteins

    49 The Primary Structure of DNA Polymerase I of E. coli

    50 DNA Polymerase I-Dependent Replication

    51 Isolation of DNA-Dependent ATPase I Mutants of E. coli

    52 Studies with the Escherichia coli dnaJ and dnaK Genes

    53 Interaction of Bacteriophage Lambda Replication Proteins with Host Cell Components

    VII. Eukaryotic Replication Enzymology

    54 Rat Liver DNA Binding Protein: Properties and Physiological Variations

    55 Characterization of Mammalian DNase V, a Double-Strand Bidirectional Exonuclease which Interacts with DNA Polymerase-β of the Novikoff Hepatoma

    56 Structure of DNA Polymerase-α

    57 KB Cell DNA Polymerase-α: Mechanism of Primer-Template Recognition by the Core Catalytic Unit

    58 Diadenosine Tetraphosphate-A Ligand of DNA Polymerase-α and Trigger of DNA Replication

    59 Activation of the Drosophila Embryo DNA Polymerase by Proteolytic Cleavage

    VIII. Studies on the Fidelity of DNA Replication

    60 On the Molecular Basis of Mutagenesis: Enzymological and Genetic Studies with the Bacteriophage T4 System

    61 Probing the Mechanism of Transition and Transversion Mutagenesis Using the Phage T4 DNA Replication Apparatus in Vitro 707

    62 Studies of the Effects of Chemical Mutagens on the Fidelity of DNA Replication

    63 Fidelity of Copying Natural DNA Templates

    64 Base Mispairing and Nearest-Neighbor Effects in Transition Mutations

    IX. DNA Topoisomerases

    65 DNA Topoisomerases

    66 Mechanisms of DNA Topoisomerases

    67 Topoisomerases and the Swivel Problem

    68 DNA Strand Passing and the Function of Type II DNA Topoisomerases

    69 Isolation of an E. coli DNA Topoisomerase I Mutant

    X. Studies of Genetic Recombination Mechanisms

    70 Genetic Recombination and the Fusion of DNA Molecules

    71 rec A Protein of E. coli Promotes Homologous Pairing of DNA Molecules by a Novel Mechanism

    72 Initiation of Genetic Recombination: Joining of Duplex DNA Molecules Promoted by rec A Protein

    73 rec A-Dependent DNA Replication in E. coli: A New Mutation Affecting the Ability of recA to Regulate DNA Replication

    74 A View of the recBC and rec F Pathways of E. coli Recombination

    75 Electron Microscopy of E. coli recBC Enzyme Reaction Intermediates

    76 Unwinding and Rewinding of DNA by Exonuclease V

    77 What Accounts for the Orientation Dependence and Directionality of Chi?

    78 Nucleotide Sequence of Chi Recombinational Hotspots

    79 Genetic Recombination of Bacterial Plasmid DNAs in Vivo and in Vitro

    80 Genetic Recombination of Bacteriophage T7 DNA in Vitro Synthesis

    81 Catenation of the Products of Integrative Recombination: Comparison of Supertwisted and Nonsupertwisted Substrates

    82 PI Site-Specific Recombination

    83 Genetic Analysis of Transposition and Excision by Transposon Tn10

    84 Tn 554: Site Specificity of Insertion and Characterization of Secondary Site Insertions


Product details

  • No. of pages: 1034
  • Language: English
  • Copyright: © Academic Press 1980
  • Published: January 1, 1980
  • Imprint: Academic Press
  • eBook ISBN: 9780323141796

About the Editor

Bruce Alberts

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