Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination - 1st Edition - ISBN: 9780128144299

Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination, Volume 600

1st Edition

Serial Volume Editors: Maria Spies Anna Malkova
Hardcover ISBN: 9780128144299
Imprint: Academic Press
Page Count: 412
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Table of Contents

  1. DSB processing in yeast
    Loraine Symington
  2. Purification of DNA end resection enzymes
    Petr Ceijka
  3. Biochemistry of DNA end resection
    Petr Ceijka
  4. Visualization of telomere movement and interchromosomal homology searches in ALT
    Roger Greenberg
  5. Pre-steady state kinetic methods for investigating the DNA exonuclease activity of Mre11
    Scott Nelson
  6. Production of large quantities of human RAD51
    Maria Spies
  7. Determining the RAD51 nucleoprotein filamentous structure and mechanism by CryoEM
    Hong-Wei Wang
  8. Single-molecule TIRF analysis of human RAD51 nucleation
    Maria Spies
  9. TIRF-based single-molecule detection of the RecA presynaptic filament dynamics
    Sung Hyun Kim
  10. Archaeal (Sulpholobus) RadA and its paralogs
    Cynthia Haseltine
  11. 4-strand DNA strand exchange
    Alexander Mazin
  12. Purification and biochemistry of DMC1 recombinase
    Doug Bishop
  13. Single-molecule TIRF analysis of yeast Rad51 nucleoprotein disassembly by helicases
    Sua Myong
  14. The recombination mediator BRCA2: architectural plasticity of recombination intermediates revealed by single molecule imaging (SFM/TIRF)
    Claire Wyman
  15. Visualisation and quantitation of BRCA2/RAD51 dynamics in cells
    Roland Kanaar
  16. RPA - DNA curtains
    Eric Greene
  17. Single-molecule studies of ssDNA binding proteins
    Taekjip Ha
  18. BRCA2 as recombination mediator
    Aura Carreira
  19. Ustilago maydis Brh2 as a model for human BRCA2
    Bill Holloman
  20. Purification and analysis of holiday junction resolvases
    Stephen West
  21. Structural analysis of HJ resolving enzyme GEN1
    David Lilley
  22. The preparation of four-stranded recombination intermediates for use in the HJ resolution reactions 
    Stephen West

Description

Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination, Volume 600, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume is the first part on mechanisms of DNA recombination and genome rearrangements.

Key Features

  • Presents an updated volume in this regular series
  • Covers research  DNA recombination and genome rearrangements
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


    Details

    No. of pages:
    412
    Copyright:
    © Academic Press 2018
    Imprint:
    Academic Press
    Hardcover ISBN:
    9780128144299

    Reviews

    Praise for the Series:
    "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
    "The work most often consulted in the lab." --Enzymologia
    "The Methods in Enzymology series represents the gold-standard." --Neuroscience


    About the Serial Volume Editors

    Maria Spies Serial Volume Editor

    Associate Professor of Biochemistry, Carver College of Medicine, University of Iowa, USA

    Affiliations and Expertise

    Carver College of Medicine, University of Iowa, USA

    Anna Malkova Serial Volume Editor

    Anna Malkova is Associate Professor of Biology at University of Iowa, USA.

    Affiliations and Expertise

    Department of Biology, University of Iowa, Iowa City, Iowa, USA